Abstract

Vitis thunbergii Sieb. et Zucc. var. taiwaniana Lu (VT) is an indigenous plant in Taiwan that is traditionally used for promoting joint health. In this study, we used in vitro primary human chondrocytes (PHCs) and two in vivo animal models to evaluate the anti-inflammatory effects of VT on arthritis. Results showed that the water extract of the stems and roots from VT (VT-SR) was rich in flavones and phenols with 1.1mg/g of resveratrol, 6.7mg/g of hopeaphenol, and 5.1mg/g of (+)-viniferin. VT-SR significantly scavenged DPPH radicals and inhibited prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-induced PHCs without exhibiting significant cytotoxicity. In in vivo models, the VT-SR (500mg/kg) significantly decreased serum PGE2 and knee 2-18F-fluoro-2-deoxy-D-glucose ( 18F-FDG) levels in LPS-induced acute inflammatory arthritis in rabbits. In addition, dietary supplementation with VT-SR for 28 days significantly alleviated type II collagenase-induced rat osteoarthritis with improvements in weight bearing and range of motion tests. In conclusion, our results suggest that the VT-SR is a good candidate for developing dietary supplements to prevent joint deterioration and inhibit inflammation.

Original languageEnglish
Pages (from-to)479-486
Number of pages8
JournalJournal of Medicinal Food
Volume17
Issue number4
DOIs
Publication statusPublished - Apr 1 2014

Fingerprint

Vitis
Chondrocytes
Dietary Supplements
Dinoprostone
Arthritis
Lipopolysaccharides
Knee
Anti-Inflammatory Agents
Joints
Flavones
Phenols
Fluorodeoxyglucose F18
Weight-Bearing
Collagenases
Articular Range of Motion
Taiwan
Osteoarthritis
Animal Models
Rabbits
Inflammation

Keywords

  • 18F-FDG uptake
  • dietary supplement
  • inflammatory arthritis
  • type II collagenase
  • Vitis thunbergii var. taiwaniana

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Medicine(all)

Cite this

Anti-inflammatory effects of vitis thunbergii var. taiwaniana on knee damage associated with arthritis. / Tsai, Ching Fent; Wang, Kun Teng; Chen, Lih Geeng; Lee, Chia Jung; Tseng, Sung Hui; Wang, Ching Chiung.

In: Journal of Medicinal Food, Vol. 17, No. 4, 01.04.2014, p. 479-486.

Research output: Contribution to journalArticle

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abstract = "Vitis thunbergii Sieb. et Zucc. var. taiwaniana Lu (VT) is an indigenous plant in Taiwan that is traditionally used for promoting joint health. In this study, we used in vitro primary human chondrocytes (PHCs) and two in vivo animal models to evaluate the anti-inflammatory effects of VT on arthritis. Results showed that the water extract of the stems and roots from VT (VT-SR) was rich in flavones and phenols with 1.1mg/g of resveratrol, 6.7mg/g of hopeaphenol, and 5.1mg/g of (+)-viniferin. VT-SR significantly scavenged DPPH radicals and inhibited prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-induced PHCs without exhibiting significant cytotoxicity. In in vivo models, the VT-SR (500mg/kg) significantly decreased serum PGE2 and knee 2-18F-fluoro-2-deoxy-D-glucose ( 18F-FDG) levels in LPS-induced acute inflammatory arthritis in rabbits. In addition, dietary supplementation with VT-SR for 28 days significantly alleviated type II collagenase-induced rat osteoarthritis with improvements in weight bearing and range of motion tests. In conclusion, our results suggest that the VT-SR is a good candidate for developing dietary supplements to prevent joint deterioration and inhibit inflammation.",
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