Abstract

Background and Objectives: Recent clinical data suggested that platelet materials used in regenerative medicine exert anti-inflammatory effects. One must understand whether functionality varies among platelet preparations and also the role of the various protein compartments. Materials and Methods: Platelet-poor-plasma (PPP), platelet lysate with cell debris (PL) or cell-free (CFPL), platelet gel releasate (PGR) and solvent/detergent-treated PL (SDPL) were prepared from four apheresis platelet donations. Protein profile was examined by SDS-PAGE, and growth factors and cytokines by ELISA, multiplexed Luminex assay and cytokine array. Anti-inflammatory activity was evaluated in RAW 264·7 mouse macrophages treated for 24 h with the blood fractions followed by 24 h of stimulation with 500 ng/ml lipopolysaccharides (LPS). Inflammatory marker nitric oxide (NO) was determined by colorimetry, tumour necrosis factor (TNF)-α by ELISA and inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 by Western blotting. Results: Proteins, growth factors and cytokines composition differed among preparations. Blood fractions alone did not stimulate inflammatory markers expression. Following LPS stimulus, NO and iNOS expressions were significantly inhibited (P <0·001) by all blood fractions, but inhibition was more pronounced with SDPL. In addition, only SDPL inhibited TNF-α (P <0·001) and COX-2 expressions. Conclusions: All the plasma and platelet fractions evaluated in this study exert an anti-inflammatory effect on macrophages, suggesting that both the plasma and platelet proteomes contribute to anti-inflammation. However, the extent and nature of the anti-inflammatory action vary among products. Further studies are needed to better understand the functionality of platelet biomaterials and optimize their clinical use.

Original languageEnglish
Pages (from-to)138-147
Number of pages10
JournalVox Sanguinis
Volume109
Issue number2
DOIs
Publication statusPublished - Aug 1 2015

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Biocompatible Materials
Anti-Inflammatory Agents
Blood Platelets
Macrophages
Detergents
Cyclooxygenase 2
Cytokines
Lipopolysaccharides
Intercellular Signaling Peptides and Proteins
Nitric Oxide
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
Colorimetry
Blood Component Removal
Proteins
Regenerative Medicine
Nitric Oxide Synthase Type II
Proteome
Nitric Oxide Synthase
Polyacrylamide Gel Electrophoresis

Keywords

  • Inflammation
  • Macrophages
  • Plasma
  • Platelet
  • Platelet gel
  • Platelet lysate

ASJC Scopus subject areas

  • Hematology

Cite this

Anti-inflammatory effects of platelet biomaterials in a macrophage cellular model. / Renn, T. Y.; Kao, Y. H.; Wang, C. C.; Burnouf, T.

In: Vox Sanguinis, Vol. 109, No. 2, 01.08.2015, p. 138-147.

Research output: Contribution to journalArticle

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abstract = "Background and Objectives: Recent clinical data suggested that platelet materials used in regenerative medicine exert anti-inflammatory effects. One must understand whether functionality varies among platelet preparations and also the role of the various protein compartments. Materials and Methods: Platelet-poor-plasma (PPP), platelet lysate with cell debris (PL) or cell-free (CFPL), platelet gel releasate (PGR) and solvent/detergent-treated PL (SDPL) were prepared from four apheresis platelet donations. Protein profile was examined by SDS-PAGE, and growth factors and cytokines by ELISA, multiplexed Luminex assay and cytokine array. Anti-inflammatory activity was evaluated in RAW 264·7 mouse macrophages treated for 24 h with the blood fractions followed by 24 h of stimulation with 500 ng/ml lipopolysaccharides (LPS). Inflammatory marker nitric oxide (NO) was determined by colorimetry, tumour necrosis factor (TNF)-α by ELISA and inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 by Western blotting. Results: Proteins, growth factors and cytokines composition differed among preparations. Blood fractions alone did not stimulate inflammatory markers expression. Following LPS stimulus, NO and iNOS expressions were significantly inhibited (P <0·001) by all blood fractions, but inhibition was more pronounced with SDPL. In addition, only SDPL inhibited TNF-α (P <0·001) and COX-2 expressions. Conclusions: All the plasma and platelet fractions evaluated in this study exert an anti-inflammatory effect on macrophages, suggesting that both the plasma and platelet proteomes contribute to anti-inflammation. However, the extent and nature of the anti-inflammatory action vary among products. Further studies are needed to better understand the functionality of platelet biomaterials and optimize their clinical use.",
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T1 - Anti-inflammatory effects of platelet biomaterials in a macrophage cellular model

AU - Renn, T. Y.

AU - Kao, Y. H.

AU - Wang, C. C.

AU - Burnouf, T.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Background and Objectives: Recent clinical data suggested that platelet materials used in regenerative medicine exert anti-inflammatory effects. One must understand whether functionality varies among platelet preparations and also the role of the various protein compartments. Materials and Methods: Platelet-poor-plasma (PPP), platelet lysate with cell debris (PL) or cell-free (CFPL), platelet gel releasate (PGR) and solvent/detergent-treated PL (SDPL) were prepared from four apheresis platelet donations. Protein profile was examined by SDS-PAGE, and growth factors and cytokines by ELISA, multiplexed Luminex assay and cytokine array. Anti-inflammatory activity was evaluated in RAW 264·7 mouse macrophages treated for 24 h with the blood fractions followed by 24 h of stimulation with 500 ng/ml lipopolysaccharides (LPS). Inflammatory marker nitric oxide (NO) was determined by colorimetry, tumour necrosis factor (TNF)-α by ELISA and inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 by Western blotting. Results: Proteins, growth factors and cytokines composition differed among preparations. Blood fractions alone did not stimulate inflammatory markers expression. Following LPS stimulus, NO and iNOS expressions were significantly inhibited (P <0·001) by all blood fractions, but inhibition was more pronounced with SDPL. In addition, only SDPL inhibited TNF-α (P <0·001) and COX-2 expressions. Conclusions: All the plasma and platelet fractions evaluated in this study exert an anti-inflammatory effect on macrophages, suggesting that both the plasma and platelet proteomes contribute to anti-inflammation. However, the extent and nature of the anti-inflammatory action vary among products. Further studies are needed to better understand the functionality of platelet biomaterials and optimize their clinical use.

AB - Background and Objectives: Recent clinical data suggested that platelet materials used in regenerative medicine exert anti-inflammatory effects. One must understand whether functionality varies among platelet preparations and also the role of the various protein compartments. Materials and Methods: Platelet-poor-plasma (PPP), platelet lysate with cell debris (PL) or cell-free (CFPL), platelet gel releasate (PGR) and solvent/detergent-treated PL (SDPL) were prepared from four apheresis platelet donations. Protein profile was examined by SDS-PAGE, and growth factors and cytokines by ELISA, multiplexed Luminex assay and cytokine array. Anti-inflammatory activity was evaluated in RAW 264·7 mouse macrophages treated for 24 h with the blood fractions followed by 24 h of stimulation with 500 ng/ml lipopolysaccharides (LPS). Inflammatory marker nitric oxide (NO) was determined by colorimetry, tumour necrosis factor (TNF)-α by ELISA and inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 by Western blotting. Results: Proteins, growth factors and cytokines composition differed among preparations. Blood fractions alone did not stimulate inflammatory markers expression. Following LPS stimulus, NO and iNOS expressions were significantly inhibited (P <0·001) by all blood fractions, but inhibition was more pronounced with SDPL. In addition, only SDPL inhibited TNF-α (P <0·001) and COX-2 expressions. Conclusions: All the plasma and platelet fractions evaluated in this study exert an anti-inflammatory effect on macrophages, suggesting that both the plasma and platelet proteomes contribute to anti-inflammation. However, the extent and nature of the anti-inflammatory action vary among products. Further studies are needed to better understand the functionality of platelet biomaterials and optimize their clinical use.

KW - Inflammation

KW - Macrophages

KW - Plasma

KW - Platelet

KW - Platelet gel

KW - Platelet lysate

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