Anti-inflammatory effects of Calophyllum inophyllum L. in RAW264.7 cells

Shih Chang Tsai, Yu Hsin Liang, Jo Hua Chiang, Fon Chang Liu, Wen Hsin Lin, Shu Jen Chang, Wei Yu Lin, Chieh Hsi Wu, Jing Ru Weng

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Calophyllum inophyllum L. has been used as folk medicine in the treatment of ocular burn and it has demonstrated potential to be an anti-inflammatory agent. The aim of this study was to explore the anti-inflammatory activities of an acetone extract of C. inophyllum L. leaves (CIL). The CIL extract was tested on lipopolysaccharide (LPS)-induced RAW 264.7 cells to evaluate the effect of CIL extract on the expression of nitric oxide (NO) and inducible nitric oxide synthase (iNOS). Results showed that the CIL extract markedly suppressed the LPS-induced production of nitric oxide, as well as the expression of iNOS, cyclooxygenase (COX)-2 and nuclear factor-kappaB (NF-κB) in a dose-dependent manner. LPS-induced microRNA (miR)-146a expression was inhibited by CIL extract, while miR-155 and miR-424 expression was not affected as demonstrated using quantitative RT-PCR analysis. Taken together, these observations show that CIL extract has anti-inflammatory effect, which extends the potential application for prevention of inflammatory diseases, and its mechanism may be partially associated with blocking COX-2 and iNOS of RAW 264.7 cells.

Original languageEnglish
Pages (from-to)1096-1102
Number of pages7
JournalOncology Reports
Volume28
Issue number3
DOIs
Publication statusPublished - Sep 2012
Externally publishedYes

Fingerprint

Calophyllum
Nitric Oxide Synthase Type II
MicroRNAs
Lipopolysaccharides
Anti-Inflammatory Agents
Cyclooxygenase 2
Nitric Oxide
Eye Burns
Traditional Medicine
Acetone
Polymerase Chain Reaction
RAW 264.7 Cells
Therapeutics

Keywords

  • Anti-inflammatory effect
  • Calophyllum inophyllum L.
  • MicroRNA
  • Traditional Chinese herbs

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tsai, S. C., Liang, Y. H., Chiang, J. H., Liu, F. C., Lin, W. H., Chang, S. J., ... Weng, J. R. (2012). Anti-inflammatory effects of Calophyllum inophyllum L. in RAW264.7 cells. Oncology Reports, 28(3), 1096-1102. https://doi.org/10.3892/or.2012.1873

Anti-inflammatory effects of Calophyllum inophyllum L. in RAW264.7 cells. / Tsai, Shih Chang; Liang, Yu Hsin; Chiang, Jo Hua; Liu, Fon Chang; Lin, Wen Hsin; Chang, Shu Jen; Lin, Wei Yu; Wu, Chieh Hsi; Weng, Jing Ru.

In: Oncology Reports, Vol. 28, No. 3, 09.2012, p. 1096-1102.

Research output: Contribution to journalArticle

Tsai, SC, Liang, YH, Chiang, JH, Liu, FC, Lin, WH, Chang, SJ, Lin, WY, Wu, CH & Weng, JR 2012, 'Anti-inflammatory effects of Calophyllum inophyllum L. in RAW264.7 cells', Oncology Reports, vol. 28, no. 3, pp. 1096-1102. https://doi.org/10.3892/or.2012.1873
Tsai SC, Liang YH, Chiang JH, Liu FC, Lin WH, Chang SJ et al. Anti-inflammatory effects of Calophyllum inophyllum L. in RAW264.7 cells. Oncology Reports. 2012 Sep;28(3):1096-1102. https://doi.org/10.3892/or.2012.1873
Tsai, Shih Chang ; Liang, Yu Hsin ; Chiang, Jo Hua ; Liu, Fon Chang ; Lin, Wen Hsin ; Chang, Shu Jen ; Lin, Wei Yu ; Wu, Chieh Hsi ; Weng, Jing Ru. / Anti-inflammatory effects of Calophyllum inophyllum L. in RAW264.7 cells. In: Oncology Reports. 2012 ; Vol. 28, No. 3. pp. 1096-1102.
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