Anti-inflammatory effects of Calophyllum inophyllum L. in RAW264.7 cells

Shih Chang Tsai, Yu Hsin Liang, Jo Hua Chiang, Fon Chang Liu, Wen Hsin Lin, Shu Jen Chang, Wei Yu Lin, Chieh Hsi Wu, Jing Ru Weng

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27 Citations (Scopus)

Abstract

Calophyllum inophyllum L. has been used as folk medicine in the treatment of ocular burn and it has demonstrated potential to be an anti-inflammatory agent. The aim of this study was to explore the anti-inflammatory activities of an acetone extract of C. inophyllum L. leaves (CIL). The CIL extract was tested on lipopolysaccharide (LPS)-induced RAW 264.7 cells to evaluate the effect of CIL extract on the expression of nitric oxide (NO) and inducible nitric oxide synthase (iNOS). Results showed that the CIL extract markedly suppressed the LPS-induced production of nitric oxide, as well as the expression of iNOS, cyclooxygenase (COX)-2 and nuclear factor-kappaB (NF-κB) in a dose-dependent manner. LPS-induced microRNA (miR)-146a expression was inhibited by CIL extract, while miR-155 and miR-424 expression was not affected as demonstrated using quantitative RT-PCR analysis. Taken together, these observations show that CIL extract has anti-inflammatory effect, which extends the potential application for prevention of inflammatory diseases, and its mechanism may be partially associated with blocking COX-2 and iNOS of RAW 264.7 cells.

Original languageEnglish
Pages (from-to)1096-1102
Number of pages7
JournalOncology Reports
Volume28
Issue number3
DOIs
Publication statusPublished - Sep 2012
Externally publishedYes

Keywords

  • Anti-inflammatory effect
  • Calophyllum inophyllum L.
  • MicroRNA
  • Traditional Chinese herbs

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Tsai, S. C., Liang, Y. H., Chiang, J. H., Liu, F. C., Lin, W. H., Chang, S. J., Lin, W. Y., Wu, C. H., & Weng, J. R. (2012). Anti-inflammatory effects of Calophyllum inophyllum L. in RAW264.7 cells. Oncology Reports, 28(3), 1096-1102. https://doi.org/10.3892/or.2012.1873