Anti-inflammatory and Antinociceptive Constituents of Atractylodes japonica Koidzumi

Lih Geeng Chen, Yun Sheng Jan, Po Wei Tsai, Hisayoshi Norimoto, Seiwa Michihara, Chiaki Murayama, Ching Chiung Wang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The rhizomes of many Atractylodes species, including Atractylodes chinensis Koidzumi, Atractylodes macrocephala Koidzumi, and Atractylodes japonica Koidzumi, are collectively termed Atractylodis Rhizoma. We prepared n-hexane extracts of the three species and evaluated their anti-inflammatory effects on lipopolysaccharide (LPS)-induced RAW 264.7 cells. Among all n-hexane extracts, those of A. japonica most strongly inhibited nitric oxide (NO) production in LPS-induced RAW 264.7 cells; five sesquiterpenes, atractylon, atractylenolide I, atractylenolide II, atractylenolide III, and 8-epiasterolid, were isolated from A. japonica. The phytochemical content of A. japonica was similar to those of A. chinensis and A. macrocephala. Moreover, the atractylon concentration was higher in A. japonica than in A. chinensis and A. macrocephala. Atractylon significantly inhibited NO and prostaglandin E2 production as well as inducible NO synthase and cyclooxygenase-2 expression in LPS-induced RAW 264.7 cells. Atractylon (40 mg/kg) also significantly reduced the acetic-acid-induced writhing response, carrageenan-induced paw edema, and hot-plate latent pain response in mice. According to the results, A. japonica has anti-inflammatory and antinociceptive effects and atractylon is the major active component of A. japonica. Therefore, atractylon can be used as a bioactivity marker in A. japonica.

Original languageEnglish
Pages (from-to)2254-2262
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number11
DOIs
Publication statusPublished - Mar 23 2016

Fingerprint

Atractylodes
Atractylodes lancea
lipopolysaccharides
Anti-Inflammatory Agents
anti-inflammatory activity
hexane
nitric oxide
Lipopolysaccharides
analgesic effect
carrageenan
extracts
prostaglandin synthase
cells
sesquiterpenoids
edema
rhizomes
phytopharmaceuticals
prostaglandins
acetic acid
pain

Keywords

  • anti-inflammation
  • antinociceptive effects
  • Atractylodes japonica
  • atractylon

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Anti-inflammatory and Antinociceptive Constituents of Atractylodes japonica Koidzumi. / Chen, Lih Geeng; Jan, Yun Sheng; Tsai, Po Wei; Norimoto, Hisayoshi; Michihara, Seiwa; Murayama, Chiaki; Wang, Ching Chiung.

In: Journal of Agricultural and Food Chemistry, Vol. 64, No. 11, 23.03.2016, p. 2254-2262.

Research output: Contribution to journalArticle

Chen, Lih Geeng ; Jan, Yun Sheng ; Tsai, Po Wei ; Norimoto, Hisayoshi ; Michihara, Seiwa ; Murayama, Chiaki ; Wang, Ching Chiung. / Anti-inflammatory and Antinociceptive Constituents of Atractylodes japonica Koidzumi. In: Journal of Agricultural and Food Chemistry. 2016 ; Vol. 64, No. 11. pp. 2254-2262.
@article{5fe5f7a270f54f10a49975cb6c63643e,
title = "Anti-inflammatory and Antinociceptive Constituents of Atractylodes japonica Koidzumi",
abstract = "The rhizomes of many Atractylodes species, including Atractylodes chinensis Koidzumi, Atractylodes macrocephala Koidzumi, and Atractylodes japonica Koidzumi, are collectively termed Atractylodis Rhizoma. We prepared n-hexane extracts of the three species and evaluated their anti-inflammatory effects on lipopolysaccharide (LPS)-induced RAW 264.7 cells. Among all n-hexane extracts, those of A. japonica most strongly inhibited nitric oxide (NO) production in LPS-induced RAW 264.7 cells; five sesquiterpenes, atractylon, atractylenolide I, atractylenolide II, atractylenolide III, and 8-epiasterolid, were isolated from A. japonica. The phytochemical content of A. japonica was similar to those of A. chinensis and A. macrocephala. Moreover, the atractylon concentration was higher in A. japonica than in A. chinensis and A. macrocephala. Atractylon significantly inhibited NO and prostaglandin E2 production as well as inducible NO synthase and cyclooxygenase-2 expression in LPS-induced RAW 264.7 cells. Atractylon (40 mg/kg) also significantly reduced the acetic-acid-induced writhing response, carrageenan-induced paw edema, and hot-plate latent pain response in mice. According to the results, A. japonica has anti-inflammatory and antinociceptive effects and atractylon is the major active component of A. japonica. Therefore, atractylon can be used as a bioactivity marker in A. japonica.",
keywords = "anti-inflammation, antinociceptive effects, Atractylodes japonica, atractylon",
author = "Chen, {Lih Geeng} and Jan, {Yun Sheng} and Tsai, {Po Wei} and Hisayoshi Norimoto and Seiwa Michihara and Chiaki Murayama and Wang, {Ching Chiung}",
year = "2016",
month = "3",
day = "23",
doi = "10.1021/acs.jafc.5b05841",
language = "English",
volume = "64",
pages = "2254--2262",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "11",

}

TY - JOUR

T1 - Anti-inflammatory and Antinociceptive Constituents of Atractylodes japonica Koidzumi

AU - Chen, Lih Geeng

AU - Jan, Yun Sheng

AU - Tsai, Po Wei

AU - Norimoto, Hisayoshi

AU - Michihara, Seiwa

AU - Murayama, Chiaki

AU - Wang, Ching Chiung

PY - 2016/3/23

Y1 - 2016/3/23

N2 - The rhizomes of many Atractylodes species, including Atractylodes chinensis Koidzumi, Atractylodes macrocephala Koidzumi, and Atractylodes japonica Koidzumi, are collectively termed Atractylodis Rhizoma. We prepared n-hexane extracts of the three species and evaluated their anti-inflammatory effects on lipopolysaccharide (LPS)-induced RAW 264.7 cells. Among all n-hexane extracts, those of A. japonica most strongly inhibited nitric oxide (NO) production in LPS-induced RAW 264.7 cells; five sesquiterpenes, atractylon, atractylenolide I, atractylenolide II, atractylenolide III, and 8-epiasterolid, were isolated from A. japonica. The phytochemical content of A. japonica was similar to those of A. chinensis and A. macrocephala. Moreover, the atractylon concentration was higher in A. japonica than in A. chinensis and A. macrocephala. Atractylon significantly inhibited NO and prostaglandin E2 production as well as inducible NO synthase and cyclooxygenase-2 expression in LPS-induced RAW 264.7 cells. Atractylon (40 mg/kg) also significantly reduced the acetic-acid-induced writhing response, carrageenan-induced paw edema, and hot-plate latent pain response in mice. According to the results, A. japonica has anti-inflammatory and antinociceptive effects and atractylon is the major active component of A. japonica. Therefore, atractylon can be used as a bioactivity marker in A. japonica.

AB - The rhizomes of many Atractylodes species, including Atractylodes chinensis Koidzumi, Atractylodes macrocephala Koidzumi, and Atractylodes japonica Koidzumi, are collectively termed Atractylodis Rhizoma. We prepared n-hexane extracts of the three species and evaluated their anti-inflammatory effects on lipopolysaccharide (LPS)-induced RAW 264.7 cells. Among all n-hexane extracts, those of A. japonica most strongly inhibited nitric oxide (NO) production in LPS-induced RAW 264.7 cells; five sesquiterpenes, atractylon, atractylenolide I, atractylenolide II, atractylenolide III, and 8-epiasterolid, were isolated from A. japonica. The phytochemical content of A. japonica was similar to those of A. chinensis and A. macrocephala. Moreover, the atractylon concentration was higher in A. japonica than in A. chinensis and A. macrocephala. Atractylon significantly inhibited NO and prostaglandin E2 production as well as inducible NO synthase and cyclooxygenase-2 expression in LPS-induced RAW 264.7 cells. Atractylon (40 mg/kg) also significantly reduced the acetic-acid-induced writhing response, carrageenan-induced paw edema, and hot-plate latent pain response in mice. According to the results, A. japonica has anti-inflammatory and antinociceptive effects and atractylon is the major active component of A. japonica. Therefore, atractylon can be used as a bioactivity marker in A. japonica.

KW - anti-inflammation

KW - antinociceptive effects

KW - Atractylodes japonica

KW - atractylon

UR - http://www.scopus.com/inward/record.url?scp=84961911048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961911048&partnerID=8YFLogxK

U2 - 10.1021/acs.jafc.5b05841

DO - 10.1021/acs.jafc.5b05841

M3 - Article

C2 - 26919689

AN - SCOPUS:84961911048

VL - 64

SP - 2254

EP - 2262

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 11

ER -