Anti-inflammatory activities of Cinnamomum cassia constituents in vitro and in vivo

Jung Chun Liao, Jeng Shyan Deng, Chuan Sung Chiu, Wen Chi Hou, Shyh Shyun Huang, Pei Hsin Shie, Guang Jhong Huang

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

We have investigated the anti-inflammatory effects of Cinnamomum cassia constituents (cinnamic aldehyde, cinnamic alcohol, cinnamic acid, and coumarin) using lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) and carrageenan (Carr)-induced mouse paw edema model. When RAW264.7 macrophages were treated with cinnamic aldehyde together with LPS, a significant concentration-dependent inhibition of nitric oxide (NO), tumor necrosis factor (TNF-α), and prostaglandin E2 (PGE 2) levels productions were detected. Western blotting revealed that cinnamic aldehyde blocked protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear transcription factor kappa B (NF-B), and IB, significantly. In the anti-inflammatory test, cinnamic aldehyde decreased the paw edema after Carr administration, and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the paw tissue. We also demonstrated cinnamic aldehyde attenuated the malondialdehyde (MDA) level and myeloperoxidase (MPO) activity in the edema paw after Carr injection. Cinnamic aldehyde decreased the NO, TNF-α, and PGE2 levels on the serum level after Carr injection. Western blotting revealed that cinnamic aldehyde decreased Carr-induced iNOS, COX-2, and NF-κB expressions in the edema paw. These findings demonstrated that cinnamic aldehyde has excellent anti-inflammatory activities and thus has great potential to be used as a source for natural health products.

Original languageEnglish
Article number429320
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012

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Cinnamomum aromaticum
Anti-Inflammatory Agents
Carrageenan
Edema
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Dinoprostone
Lipopolysaccharides
Nitric Oxide
Tumor Necrosis Factor-alpha
Western Blotting
Macrophages
Injections
cinnamic aldehyde
In Vitro Techniques
NF-kappa B
Glutathione Peroxidase
Malondialdehyde
Catalase
Peroxidase

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Anti-inflammatory activities of Cinnamomum cassia constituents in vitro and in vivo. / Liao, Jung Chun; Deng, Jeng Shyan; Chiu, Chuan Sung; Hou, Wen Chi; Huang, Shyh Shyun; Shie, Pei Hsin; Huang, Guang Jhong.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 429320, 2012.

Research output: Contribution to journalArticle

Liao, Jung Chun ; Deng, Jeng Shyan ; Chiu, Chuan Sung ; Hou, Wen Chi ; Huang, Shyh Shyun ; Shie, Pei Hsin ; Huang, Guang Jhong. / Anti-inflammatory activities of Cinnamomum cassia constituents in vitro and in vivo. In: Evidence-based Complementary and Alternative Medicine. 2012 ; Vol. 2012.
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