Anti-glycation, anti-hemolysis, and ORAC activities of demethylcurcumin and tetrahydroxycurcumin in vitro and reductions of oxidative stress in d-galactose-induced BALB/c mice in vivo

Yuh Hwa Liu, Tai Lin Lee, Chuan Hsiao Han, Yi Shan Lee, Wen Chi Hou

Research output: Contribution to journalArticle

Abstract

Background: There were few report concerning anti-glycation and antioxidant activities of the minor amounts of components in curcuminoids, demethylcurcumin and tetrahydroxycurcumin, in vitro and in vivo. Results: The bovine serum albumin/galactose of non-enzymatic glycation models, radical-induced hemolysis, and oxygen radical absorbance capacity (ORAC) were studied in vitro, and the d-galactose-induced oxidative stress in BALB/c mice and then demethylcurcumin or tetrahydroxycurcumin interventions in vivo. The parameters of oxidative stress in plasma and brain extracts were determined among animal groups with or without both curcuminoids interventions. The demethylcurcumin and tetrahydroxycurcumin exhibited anti-glycation, anti-hemolysis, and ORAC activities, and showed much better and significant difference (P < 0.05) compared to those of curcumin in vitro. In animal experiments, the intervened two curcuminoids at both concentrations showed to lower serum malondialdehyde (MDA), brain MDA levels and iNOS protein expressions, and elevate serum ORAC activities, and showed difference (P < 0.05) compared to the galactose-induced control. Conclusion: The demethylcurcumin and tetrahydroxycurcumin showed potentials in developing functional foods for antioxidant-related purposes.

Original languageEnglish
Article number9
JournalBotanical Studies
Volume60
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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oxygen radical absorbance capacity
glycation
hemolysis
galactose
oxidative stress
malondialdehyde
mice
brain
curcumin
animal experimentation
bovine serum albumin
functional foods
protein synthesis
antioxidant activity
antioxidants
extracts
animals

Keywords

  • Antioxidant
  • Curcuminoids
  • Glycation
  • Hemolytic
  • ORAC

ASJC Scopus subject areas

  • Plant Science

Cite this

Anti-glycation, anti-hemolysis, and ORAC activities of demethylcurcumin and tetrahydroxycurcumin in vitro and reductions of oxidative stress in d-galactose-induced BALB/c mice in vivo. / Liu, Yuh Hwa; Lee, Tai Lin; Han, Chuan Hsiao; Lee, Yi Shan; Hou, Wen Chi.

In: Botanical Studies, Vol. 60, No. 1, 9, 01.12.2019.

Research output: Contribution to journalArticle

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