Anti-CCR3 monoclonal antibody inhibits eosinophil infiltration in Angiostrongylus cantonensis-infected ICR mice

Chih Cheng Chuang, Kua Eyre Su, Chi W. Chen, Chia Kwung Fan, Feng Kuan Lin, Yu Shen Chen, Wen Yuan Du

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9 Citations (Scopus)

Abstract

ICR mice were each infected with 35 Angiostrongylus cantonensis larvae. One group of mice received an intraperitoneal injection of anti-CCR3 monoclonal antibody (mAb) (50μg) at 10 days post-infection (dpi), while another similarly-treated group also received a booster injection (25μg) at 12. dpi. All the mice were sacrificed at 14. dpi for pathological examination, enzyme-linked immunosorbent assay analysis and RNA extraction. The infiltration of eosinophils and the severity of eosinophilic meningitis were reduced in both the mAb-treated groups, relative to infected but untreated animals. The levels of CCL11 (eotaxin) in the peripheral circulation and the expression of the Th2-type cytokine interleukin-5 in the brains were significantly reduced. A. cantonensis infection is the major cause of eosinophilic meningoencephalitis in Taiwan, and the results of this study could be useful for the development of strategies to reduce the neurological damage caused by this infection.

Original languageEnglish
Pages (from-to)209-213
Number of pages5
JournalActa Tropica
Volume113
Issue number3
DOIs
Publication statusPublished - Mar 2010

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Keywords

  • Angiostrongylus cantonensis
  • CCR3

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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