Anti-cancer activity of Solanum nigrum (AESN) through suppression of mitochondrial function and epithelial-mesenchymal transition (EMT) in breast cancer cells

Ying Jang Lai, Chen Jei Tai, Chia Woei Wang, Chen Yen Choong, Bao Hong Lee, Yeu Ching Shi, Cheng Jeng Tai

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Chemotherapy is the main approach for treating advanced and recurrent carcinoma, but the clinical performance of chemotherapy is limited by relatively low response rates, drug resistance, and adverse effects that severely affect the quality of life of patients. An association between epithelial-mesenchymal transition (EMT) and chemotherapy resistance has been investigated in recent studies. Our recent studies have found that the aqueous extract of Solanum nigrum (AESN) is a crucial ingredient in some traditional Chinese medicine formulas for treating various types of cancer patients and exhibits antitumor effects. We evaluated the suppression of EMT in MCF-7 breast cancer cells treated with AESN. The mitochondrial morphology was investigated using Mitotracker Deep-Red FM stain. Our results indicated that AESN markedly inhibited cell viability of MCF-7 breast cancer cells through apoptosis induction and cell cycle arrest mediated by activation of caspase-3 and production of reactive oxygen species. Furthermore, mitochondrial fission was observed in MCF-7 breast cancer cells treated with AESN. In addition to elevation of E-cadherin, downregulations of ZEB1, N-cadherin, and vimentin were found in AESN-treated MCF-7 breast cancer cells. These results suggested that AESN could inhibit EMT of MCF-7 breast cancer cells mediated by attenuation of mitochondrial function. AESN could be potentially beneficial in treating breast cancer cells, and may be of interest for future studies in developing integrative cancer therapy against proliferation, metastasis, and migration of breast cancer cells.

Original languageEnglish
Article number553
JournalMolecules
Volume21
Issue number5
DOIs
Publication statusPublished - May 1 2016

Fingerprint

Solanum nigrum
Epithelial-Mesenchymal Transition
breast
cancer
Cells
retarding
Breast Neoplasms
Neoplasms
Chemotherapy
chemotherapy
Cadherins
Drug Therapy
Mitochondrial Dynamics
Chinese Traditional Medicine
Vimentin
Cell Cycle Checkpoints
Drug Resistance
Caspase 3
Reactive Oxygen Species
Cell Survival

Keywords

  • Apoptosis
  • Aqueous extracts of Solanum nigrum (AESN)
  • Epithelial-mesenchymal transition (EMT)
  • MCF-7 breast cancer cells
  • Mitochondrial fission

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Anti-cancer activity of Solanum nigrum (AESN) through suppression of mitochondrial function and epithelial-mesenchymal transition (EMT) in breast cancer cells. / Lai, Ying Jang; Tai, Chen Jei; Wang, Chia Woei; Choong, Chen Yen; Lee, Bao Hong; Shi, Yeu Ching; Tai, Cheng Jeng.

In: Molecules, Vol. 21, No. 5, 553, 01.05.2016.

Research output: Contribution to journalArticle

@article{cacac5301b78486ab40a1f48a4ca2859,
title = "Anti-cancer activity of Solanum nigrum (AESN) through suppression of mitochondrial function and epithelial-mesenchymal transition (EMT) in breast cancer cells",
abstract = "Chemotherapy is the main approach for treating advanced and recurrent carcinoma, but the clinical performance of chemotherapy is limited by relatively low response rates, drug resistance, and adverse effects that severely affect the quality of life of patients. An association between epithelial-mesenchymal transition (EMT) and chemotherapy resistance has been investigated in recent studies. Our recent studies have found that the aqueous extract of Solanum nigrum (AESN) is a crucial ingredient in some traditional Chinese medicine formulas for treating various types of cancer patients and exhibits antitumor effects. We evaluated the suppression of EMT in MCF-7 breast cancer cells treated with AESN. The mitochondrial morphology was investigated using Mitotracker Deep-Red FM stain. Our results indicated that AESN markedly inhibited cell viability of MCF-7 breast cancer cells through apoptosis induction and cell cycle arrest mediated by activation of caspase-3 and production of reactive oxygen species. Furthermore, mitochondrial fission was observed in MCF-7 breast cancer cells treated with AESN. In addition to elevation of E-cadherin, downregulations of ZEB1, N-cadherin, and vimentin were found in AESN-treated MCF-7 breast cancer cells. These results suggested that AESN could inhibit EMT of MCF-7 breast cancer cells mediated by attenuation of mitochondrial function. AESN could be potentially beneficial in treating breast cancer cells, and may be of interest for future studies in developing integrative cancer therapy against proliferation, metastasis, and migration of breast cancer cells.",
keywords = "Apoptosis, Aqueous extracts of Solanum nigrum (AESN), Epithelial-mesenchymal transition (EMT), MCF-7 breast cancer cells, Mitochondrial fission",
author = "Lai, {Ying Jang} and Tai, {Chen Jei} and Wang, {Chia Woei} and Choong, {Chen Yen} and Lee, {Bao Hong} and Shi, {Yeu Ching} and Tai, {Cheng Jeng}",
year = "2016",
month = "5",
day = "1",
doi = "10.3390/molecules21050553",
language = "English",
volume = "21",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "5",

}

TY - JOUR

T1 - Anti-cancer activity of Solanum nigrum (AESN) through suppression of mitochondrial function and epithelial-mesenchymal transition (EMT) in breast cancer cells

AU - Lai, Ying Jang

AU - Tai, Chen Jei

AU - Wang, Chia Woei

AU - Choong, Chen Yen

AU - Lee, Bao Hong

AU - Shi, Yeu Ching

AU - Tai, Cheng Jeng

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Chemotherapy is the main approach for treating advanced and recurrent carcinoma, but the clinical performance of chemotherapy is limited by relatively low response rates, drug resistance, and adverse effects that severely affect the quality of life of patients. An association between epithelial-mesenchymal transition (EMT) and chemotherapy resistance has been investigated in recent studies. Our recent studies have found that the aqueous extract of Solanum nigrum (AESN) is a crucial ingredient in some traditional Chinese medicine formulas for treating various types of cancer patients and exhibits antitumor effects. We evaluated the suppression of EMT in MCF-7 breast cancer cells treated with AESN. The mitochondrial morphology was investigated using Mitotracker Deep-Red FM stain. Our results indicated that AESN markedly inhibited cell viability of MCF-7 breast cancer cells through apoptosis induction and cell cycle arrest mediated by activation of caspase-3 and production of reactive oxygen species. Furthermore, mitochondrial fission was observed in MCF-7 breast cancer cells treated with AESN. In addition to elevation of E-cadherin, downregulations of ZEB1, N-cadherin, and vimentin were found in AESN-treated MCF-7 breast cancer cells. These results suggested that AESN could inhibit EMT of MCF-7 breast cancer cells mediated by attenuation of mitochondrial function. AESN could be potentially beneficial in treating breast cancer cells, and may be of interest for future studies in developing integrative cancer therapy against proliferation, metastasis, and migration of breast cancer cells.

AB - Chemotherapy is the main approach for treating advanced and recurrent carcinoma, but the clinical performance of chemotherapy is limited by relatively low response rates, drug resistance, and adverse effects that severely affect the quality of life of patients. An association between epithelial-mesenchymal transition (EMT) and chemotherapy resistance has been investigated in recent studies. Our recent studies have found that the aqueous extract of Solanum nigrum (AESN) is a crucial ingredient in some traditional Chinese medicine formulas for treating various types of cancer patients and exhibits antitumor effects. We evaluated the suppression of EMT in MCF-7 breast cancer cells treated with AESN. The mitochondrial morphology was investigated using Mitotracker Deep-Red FM stain. Our results indicated that AESN markedly inhibited cell viability of MCF-7 breast cancer cells through apoptosis induction and cell cycle arrest mediated by activation of caspase-3 and production of reactive oxygen species. Furthermore, mitochondrial fission was observed in MCF-7 breast cancer cells treated with AESN. In addition to elevation of E-cadherin, downregulations of ZEB1, N-cadherin, and vimentin were found in AESN-treated MCF-7 breast cancer cells. These results suggested that AESN could inhibit EMT of MCF-7 breast cancer cells mediated by attenuation of mitochondrial function. AESN could be potentially beneficial in treating breast cancer cells, and may be of interest for future studies in developing integrative cancer therapy against proliferation, metastasis, and migration of breast cancer cells.

KW - Apoptosis

KW - Aqueous extracts of Solanum nigrum (AESN)

KW - Epithelial-mesenchymal transition (EMT)

KW - MCF-7 breast cancer cells

KW - Mitochondrial fission

UR - http://www.scopus.com/inward/record.url?scp=84973457901&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973457901&partnerID=8YFLogxK

U2 - 10.3390/molecules21050553

DO - 10.3390/molecules21050553

M3 - Article

C2 - 27136519

AN - SCOPUS:84973457901

VL - 21

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 5

M1 - 553

ER -