Anti-apoptotic gene delivery with cyclo-(D-Trp-Tyr) peptide nanotube via eye drop following corneal epithelial debridement

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Corneal keratocyte apoptosis triggered by cornel debridement is one mechanism of corneal disorders. In this study, the feasibility of cyclo-(D-Trp-Tyr) peptide nanotubes (PNTs) as carriers of caspase 3 silence shRNA delivery was assessed. A model of epithelial injury by epithelial debridement was applied to investigate the feasibility of PNTs as gene delivery carriers on corneal injury. First, the PNTs were found within 2 μm in length and 300 nm in width by an atomic force microscope and confocal laser microscope system. Plasmid DNAs were observed to be associated with PNTs by atomic force microscope and confocal laser scanning microscope. The plasmids were associated with tyrosine of PNTs with a binding constant of 2.7 × 108 M−1. The stability of plasmid DNA with PNTs against the DNase was found at 60 min. Using thioflavin T pre-stained PNTs on the corneal eye drop delivery, the distribution of PNTs was in the epithelial and stroma regions. After corneal debridement, the rhodamine-labeled plasmid DNA and thioflavin T pre-stained PNTs were also delivered and could be observed in the stroma of cornea. PNTs complexed with anti-apoptotic plasmid caspase 3 silencing shRNA eye drop delivery decreased 41% of caspase 3 activity after the first dose by caspase 3 activity and Western blot analysis.

Original languageEnglish
Pages (from-to)122-136
Number of pages15
JournalPharmaceutics
Volume7
Issue number3
DOIs
Publication statusPublished - Jul 17 2015

Fingerprint

tryptophyltyrosine
Peptide Nanotubes
Ophthalmic Solutions
Debridement
Genes
Plasmids
Caspase 3
Small Interfering RNA
DNA
Lasers
Corneal Keratocytes
Rhodamines
Deoxyribonucleases

Keywords

  • Caspase 3
  • Cornea debridement
  • Peptide nanotube

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Anti-apoptotic gene delivery with cyclo-(D-Trp-Tyr) peptide nanotube via eye drop following corneal epithelial debridement. / Lee, Yu Hsing; Chang, Shwu Fen; Liaw, Jiahorng.

In: Pharmaceutics, Vol. 7, No. 3, 17.07.2015, p. 122-136.

Research output: Contribution to journalArticle

@article{609ca1db79b6413f83a55d3f48beddd7,
title = "Anti-apoptotic gene delivery with cyclo-(D-Trp-Tyr) peptide nanotube via eye drop following corneal epithelial debridement",
abstract = "Corneal keratocyte apoptosis triggered by cornel debridement is one mechanism of corneal disorders. In this study, the feasibility of cyclo-(D-Trp-Tyr) peptide nanotubes (PNTs) as carriers of caspase 3 silence shRNA delivery was assessed. A model of epithelial injury by epithelial debridement was applied to investigate the feasibility of PNTs as gene delivery carriers on corneal injury. First, the PNTs were found within 2 μm in length and 300 nm in width by an atomic force microscope and confocal laser microscope system. Plasmid DNAs were observed to be associated with PNTs by atomic force microscope and confocal laser scanning microscope. The plasmids were associated with tyrosine of PNTs with a binding constant of 2.7 × 108 M−1. The stability of plasmid DNA with PNTs against the DNase was found at 60 min. Using thioflavin T pre-stained PNTs on the corneal eye drop delivery, the distribution of PNTs was in the epithelial and stroma regions. After corneal debridement, the rhodamine-labeled plasmid DNA and thioflavin T pre-stained PNTs were also delivered and could be observed in the stroma of cornea. PNTs complexed with anti-apoptotic plasmid caspase 3 silencing shRNA eye drop delivery decreased 41{\%} of caspase 3 activity after the first dose by caspase 3 activity and Western blot analysis.",
keywords = "Caspase 3, Cornea debridement, Peptide nanotube",
author = "Lee, {Yu Hsing} and Chang, {Shwu Fen} and Jiahorng Liaw",
year = "2015",
month = "7",
day = "17",
doi = "10.3390/pharmaceutics7030122",
language = "English",
volume = "7",
pages = "122--136",
journal = "Pharmaceutics",
issn = "1999-4923",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "3",

}

TY - JOUR

T1 - Anti-apoptotic gene delivery with cyclo-(D-Trp-Tyr) peptide nanotube via eye drop following corneal epithelial debridement

AU - Lee, Yu Hsing

AU - Chang, Shwu Fen

AU - Liaw, Jiahorng

PY - 2015/7/17

Y1 - 2015/7/17

N2 - Corneal keratocyte apoptosis triggered by cornel debridement is one mechanism of corneal disorders. In this study, the feasibility of cyclo-(D-Trp-Tyr) peptide nanotubes (PNTs) as carriers of caspase 3 silence shRNA delivery was assessed. A model of epithelial injury by epithelial debridement was applied to investigate the feasibility of PNTs as gene delivery carriers on corneal injury. First, the PNTs were found within 2 μm in length and 300 nm in width by an atomic force microscope and confocal laser microscope system. Plasmid DNAs were observed to be associated with PNTs by atomic force microscope and confocal laser scanning microscope. The plasmids were associated with tyrosine of PNTs with a binding constant of 2.7 × 108 M−1. The stability of plasmid DNA with PNTs against the DNase was found at 60 min. Using thioflavin T pre-stained PNTs on the corneal eye drop delivery, the distribution of PNTs was in the epithelial and stroma regions. After corneal debridement, the rhodamine-labeled plasmid DNA and thioflavin T pre-stained PNTs were also delivered and could be observed in the stroma of cornea. PNTs complexed with anti-apoptotic plasmid caspase 3 silencing shRNA eye drop delivery decreased 41% of caspase 3 activity after the first dose by caspase 3 activity and Western blot analysis.

AB - Corneal keratocyte apoptosis triggered by cornel debridement is one mechanism of corneal disorders. In this study, the feasibility of cyclo-(D-Trp-Tyr) peptide nanotubes (PNTs) as carriers of caspase 3 silence shRNA delivery was assessed. A model of epithelial injury by epithelial debridement was applied to investigate the feasibility of PNTs as gene delivery carriers on corneal injury. First, the PNTs were found within 2 μm in length and 300 nm in width by an atomic force microscope and confocal laser microscope system. Plasmid DNAs were observed to be associated with PNTs by atomic force microscope and confocal laser scanning microscope. The plasmids were associated with tyrosine of PNTs with a binding constant of 2.7 × 108 M−1. The stability of plasmid DNA with PNTs against the DNase was found at 60 min. Using thioflavin T pre-stained PNTs on the corneal eye drop delivery, the distribution of PNTs was in the epithelial and stroma regions. After corneal debridement, the rhodamine-labeled plasmid DNA and thioflavin T pre-stained PNTs were also delivered and could be observed in the stroma of cornea. PNTs complexed with anti-apoptotic plasmid caspase 3 silencing shRNA eye drop delivery decreased 41% of caspase 3 activity after the first dose by caspase 3 activity and Western blot analysis.

KW - Caspase 3

KW - Cornea debridement

KW - Peptide nanotube

UR - http://www.scopus.com/inward/record.url?scp=84938399408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938399408&partnerID=8YFLogxK

U2 - 10.3390/pharmaceutics7030122

DO - 10.3390/pharmaceutics7030122

M3 - Article

AN - SCOPUS:84938399408

VL - 7

SP - 122

EP - 136

JO - Pharmaceutics

JF - Pharmaceutics

SN - 1999-4923

IS - 3

ER -