Anti-apoptotic effects of IGF-I on mortality and dysmorphogenesis in tbx5-deficient zebrafish embryos

Tzu Chun Tsai, Chun Che Shih, Hsin Ping Chien, An Hang Yang, Jenn Kan Lu, Jen Her Lu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Tbx5 deficiency in zebrafish causes several abnormal phenotypes of the heart and pectoral fins. It has been reported that exogenous human growth hormone can enhance expression of downstream mediators in the growth hormone and insulin-like growth factor I (IGF-I) pathway and partially restore dysmorphogenesis in tbx5 morphants. This study aimed to further evaluate the effects of IGF-I on cell apoptosis and dysmorphogenesis in zebrafish embryos deficient for tbx5. Results: Among the five studied groups of zebrafish embryos (wild-type embryos [WT], tbx5 morphants [MO], mismatched tbx5 morpholino-treated wild-type embryos [MIS], IGF-I-treated wild-type embryos [WTIGF1], and IGF-I-treated tbx5 morphants [MOIGF1]), the expression levels of the ifg1, igf1-ra, ifg-rb, erk1, and akt2 genes as well as the ERK and AKT proteins were significantly reduced in the MO group, but were partially restored in the MOIGF1 group. These expression levels remained normal in the WT, MIS, and WTIGF1 groups. Exogenous human IGF-I also reduced the incidence of phenotypic anomalies, decreased the expression levels of apoptotic genes and proteins, suppressed cell apoptosis, and improved survival of the MOIGF1 group. Conclusions: These results suggest that IGF-I has an anti-apoptotic protective effect in zebrafish embryos with tbx5 deficiency.

Original languageEnglish
Article number2
JournalBMC Developmental Biology
Volume18
Issue number1
DOIs
Publication statusPublished - Mar 5 2018
Externally publishedYes

Fingerprint

Zebrafish
Insulin-Like Growth Factor I
Embryonic Structures
Mortality
Apoptosis
Morpholinos
Human Growth Hormone
Growth Hormone
Proteins
Phenotype
Survival
Incidence
Genes

Keywords

  • Apoptosis
  • Holt-Oram syndrome
  • Insulin-like growth factor I
  • tbx5
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Anti-apoptotic effects of IGF-I on mortality and dysmorphogenesis in tbx5-deficient zebrafish embryos. / Tsai, Tzu Chun; Shih, Chun Che; Chien, Hsin Ping; Yang, An Hang; Lu, Jenn Kan; Lu, Jen Her.

In: BMC Developmental Biology, Vol. 18, No. 1, 2, 05.03.2018.

Research output: Contribution to journalArticle

Tsai, Tzu Chun ; Shih, Chun Che ; Chien, Hsin Ping ; Yang, An Hang ; Lu, Jenn Kan ; Lu, Jen Her. / Anti-apoptotic effects of IGF-I on mortality and dysmorphogenesis in tbx5-deficient zebrafish embryos. In: BMC Developmental Biology. 2018 ; Vol. 18, No. 1.
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