Anti-apoptotic and pro-survival effect of alpinate oxyphyllae fructus (AOF) in a D-galactose-induced aging heart

Yung Ming Chang, Hen Hong Chang, Wei Wen Kuo, Hung Jen Lin, Yu Lan Yeh, Vijaya Padma Viswanadha, Chin Chuan Tsai, Ray Jade Chen, Hsin Nung Chang, Chih Yang Huang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aging, a natural biological/physiological phenomenon, is accelerated by reactive oxygen species (ROS) accumulation and identified by a progressive decrease in physiological function. Several studies have shown a positive relationship between aging and chronic heart failure (HF). Cardiac apoptosis was found in age-related diseases. We used a traditional Chinese medicine, Alpinate Oxyphyllae Fructus (AOF), to evaluate its effect on cardiac anti-apoptosis and pro-survival. Male eight-week-old Sprague-Dawley (SD) rats were segregated into five groups: normal control group (NC), D-Galactose-Induced aging group (Aging), and AOF of 50 (AL (AOF low)), 100 (AM (AOF medium)), 150 (AH (AOF high)) mg/kg/day. After eight weeks, hearts were measured by an Hematoxylin-Eosin (H and E) stain, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-assays and Western blotting. The experimental results show that the cardiomyocyte apoptotic pathway protein expression increased in the D-Galactose-Induced aging groups, with dose-dependent inhibition in the AOF treatment group (AL, AM, and AH). Moreover, the expression of the pro-survival p-Akt (protein kinase B (Akt)), Bcl-2 (B-cell lymphoma 2), anti-apoptotic protein (Bcl-xL) protein decreased significantly in the D-Galactose-induced aging group, with increased performance in the AOF treatment group with levels of p-IGFIR and p-PI3K (Phosphatidylinositol-3’ kinase (PI3K)) to increase by dosage and compensatory performance. On the other hand, the protein of the Sirtuin 1 (SIRT1) pathway expression decreased in the aging groups and showed improvement in the AOF treatment group. Our results suggest that AOF strongly works against ROS-induced aging heart problems.

Original languageEnglish
Article number466
JournalInternational Journal of Molecular Sciences
Volume17
Issue number4
DOIs
Publication statusPublished - Mar 29 2016

Fingerprint

galactose
Galactose
Phosphatidylinositol 3-Kinase
Aging of materials
Reactive Oxygen Species
Sirtuin 1
Apoptosis
Physiological Phenomena
Biological Phenomena
Proteins
Proto-Oncogene Proteins c-akt
Apoptosis Regulatory Proteins
DNA Nucleotidylexotransferase
proteins
Chinese Traditional Medicine
B-Cell Lymphoma
Hematoxylin
Eosine Yellowish-(YS)
Cardiac Myocytes
Sprague Dawley Rats

Keywords

  • AOF
  • Apoptosis
  • D-galactose-induced aging
  • SIRT1

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications

Cite this

Anti-apoptotic and pro-survival effect of alpinate oxyphyllae fructus (AOF) in a D-galactose-induced aging heart. / Chang, Yung Ming; Chang, Hen Hong; Kuo, Wei Wen; Lin, Hung Jen; Yeh, Yu Lan; Viswanadha, Vijaya Padma; Tsai, Chin Chuan; Chen, Ray Jade; Chang, Hsin Nung; Huang, Chih Yang.

In: International Journal of Molecular Sciences, Vol. 17, No. 4, 466, 29.03.2016.

Research output: Contribution to journalArticle

Chang, Yung Ming ; Chang, Hen Hong ; Kuo, Wei Wen ; Lin, Hung Jen ; Yeh, Yu Lan ; Viswanadha, Vijaya Padma ; Tsai, Chin Chuan ; Chen, Ray Jade ; Chang, Hsin Nung ; Huang, Chih Yang. / Anti-apoptotic and pro-survival effect of alpinate oxyphyllae fructus (AOF) in a D-galactose-induced aging heart. In: International Journal of Molecular Sciences. 2016 ; Vol. 17, No. 4.
@article{42da750461c149b5b8c60b65b06d4ca4,
title = "Anti-apoptotic and pro-survival effect of alpinate oxyphyllae fructus (AOF) in a D-galactose-induced aging heart",
abstract = "Aging, a natural biological/physiological phenomenon, is accelerated by reactive oxygen species (ROS) accumulation and identified by a progressive decrease in physiological function. Several studies have shown a positive relationship between aging and chronic heart failure (HF). Cardiac apoptosis was found in age-related diseases. We used a traditional Chinese medicine, Alpinate Oxyphyllae Fructus (AOF), to evaluate its effect on cardiac anti-apoptosis and pro-survival. Male eight-week-old Sprague-Dawley (SD) rats were segregated into five groups: normal control group (NC), D-Galactose-Induced aging group (Aging), and AOF of 50 (AL (AOF low)), 100 (AM (AOF medium)), 150 (AH (AOF high)) mg/kg/day. After eight weeks, hearts were measured by an Hematoxylin-Eosin (H and E) stain, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-assays and Western blotting. The experimental results show that the cardiomyocyte apoptotic pathway protein expression increased in the D-Galactose-Induced aging groups, with dose-dependent inhibition in the AOF treatment group (AL, AM, and AH). Moreover, the expression of the pro-survival p-Akt (protein kinase B (Akt)), Bcl-2 (B-cell lymphoma 2), anti-apoptotic protein (Bcl-xL) protein decreased significantly in the D-Galactose-induced aging group, with increased performance in the AOF treatment group with levels of p-IGFIR and p-PI3K (Phosphatidylinositol-3’ kinase (PI3K)) to increase by dosage and compensatory performance. On the other hand, the protein of the Sirtuin 1 (SIRT1) pathway expression decreased in the aging groups and showed improvement in the AOF treatment group. Our results suggest that AOF strongly works against ROS-induced aging heart problems.",
keywords = "AOF, Apoptosis, D-galactose-induced aging, SIRT1",
author = "Chang, {Yung Ming} and Chang, {Hen Hong} and Kuo, {Wei Wen} and Lin, {Hung Jen} and Yeh, {Yu Lan} and Viswanadha, {Vijaya Padma} and Tsai, {Chin Chuan} and Chen, {Ray Jade} and Chang, {Hsin Nung} and Huang, {Chih Yang}",
year = "2016",
month = "3",
day = "29",
doi = "10.3390/ijms17040466",
language = "English",
volume = "17",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "MDPI AG",
number = "4",

}

TY - JOUR

T1 - Anti-apoptotic and pro-survival effect of alpinate oxyphyllae fructus (AOF) in a D-galactose-induced aging heart

AU - Chang, Yung Ming

AU - Chang, Hen Hong

AU - Kuo, Wei Wen

AU - Lin, Hung Jen

AU - Yeh, Yu Lan

AU - Viswanadha, Vijaya Padma

AU - Tsai, Chin Chuan

AU - Chen, Ray Jade

AU - Chang, Hsin Nung

AU - Huang, Chih Yang

PY - 2016/3/29

Y1 - 2016/3/29

N2 - Aging, a natural biological/physiological phenomenon, is accelerated by reactive oxygen species (ROS) accumulation and identified by a progressive decrease in physiological function. Several studies have shown a positive relationship between aging and chronic heart failure (HF). Cardiac apoptosis was found in age-related diseases. We used a traditional Chinese medicine, Alpinate Oxyphyllae Fructus (AOF), to evaluate its effect on cardiac anti-apoptosis and pro-survival. Male eight-week-old Sprague-Dawley (SD) rats were segregated into five groups: normal control group (NC), D-Galactose-Induced aging group (Aging), and AOF of 50 (AL (AOF low)), 100 (AM (AOF medium)), 150 (AH (AOF high)) mg/kg/day. After eight weeks, hearts were measured by an Hematoxylin-Eosin (H and E) stain, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-assays and Western blotting. The experimental results show that the cardiomyocyte apoptotic pathway protein expression increased in the D-Galactose-Induced aging groups, with dose-dependent inhibition in the AOF treatment group (AL, AM, and AH). Moreover, the expression of the pro-survival p-Akt (protein kinase B (Akt)), Bcl-2 (B-cell lymphoma 2), anti-apoptotic protein (Bcl-xL) protein decreased significantly in the D-Galactose-induced aging group, with increased performance in the AOF treatment group with levels of p-IGFIR and p-PI3K (Phosphatidylinositol-3’ kinase (PI3K)) to increase by dosage and compensatory performance. On the other hand, the protein of the Sirtuin 1 (SIRT1) pathway expression decreased in the aging groups and showed improvement in the AOF treatment group. Our results suggest that AOF strongly works against ROS-induced aging heart problems.

AB - Aging, a natural biological/physiological phenomenon, is accelerated by reactive oxygen species (ROS) accumulation and identified by a progressive decrease in physiological function. Several studies have shown a positive relationship between aging and chronic heart failure (HF). Cardiac apoptosis was found in age-related diseases. We used a traditional Chinese medicine, Alpinate Oxyphyllae Fructus (AOF), to evaluate its effect on cardiac anti-apoptosis and pro-survival. Male eight-week-old Sprague-Dawley (SD) rats were segregated into five groups: normal control group (NC), D-Galactose-Induced aging group (Aging), and AOF of 50 (AL (AOF low)), 100 (AM (AOF medium)), 150 (AH (AOF high)) mg/kg/day. After eight weeks, hearts were measured by an Hematoxylin-Eosin (H and E) stain, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-assays and Western blotting. The experimental results show that the cardiomyocyte apoptotic pathway protein expression increased in the D-Galactose-Induced aging groups, with dose-dependent inhibition in the AOF treatment group (AL, AM, and AH). Moreover, the expression of the pro-survival p-Akt (protein kinase B (Akt)), Bcl-2 (B-cell lymphoma 2), anti-apoptotic protein (Bcl-xL) protein decreased significantly in the D-Galactose-induced aging group, with increased performance in the AOF treatment group with levels of p-IGFIR and p-PI3K (Phosphatidylinositol-3’ kinase (PI3K)) to increase by dosage and compensatory performance. On the other hand, the protein of the Sirtuin 1 (SIRT1) pathway expression decreased in the aging groups and showed improvement in the AOF treatment group. Our results suggest that AOF strongly works against ROS-induced aging heart problems.

KW - AOF

KW - Apoptosis

KW - D-galactose-induced aging

KW - SIRT1

UR - http://www.scopus.com/inward/record.url?scp=84962090217&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962090217&partnerID=8YFLogxK

U2 - 10.3390/ijms17040466

DO - 10.3390/ijms17040466

M3 - Article

C2 - 27043531

AN - SCOPUS:84962090217

VL - 17

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 4

M1 - 466

ER -