Anti-ageing effects of alpha-naphthoflavone on normal and UVB-irradiated human skin fibroblasts

Po Lin Liao, Ching Hao Li, Cheng Yi Chang, Siang Rong Lu, Cheng Hui Lin, Ling Shan Tse, Yu Wen Cheng

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Ageing is a complex and multifactorial process resulting in several functional and aesthetic changes to the skin. We found that α-Naphthoflavone (α-NF) concentration-dependently induced pro-collagen type I protein expression and inhibited MMP-1 protein expression, in both normal and UVB-irradiated cells. SB431542 and SIS3 - inhibitors of TGF-β and Smad3, respectively - significantly alleviate α-NF-caused response of MMP-1 and pro-collagen. LY294002 (PI3K inhibitor) can reverse α-NF-induced ERK, Akt, Smad-3 activation, pro-collagen synthesis and α-NF-suppressed AP-1 activation. PD (ERK inhibitor) was not involved in pro-collagen generation and MMP-1 inhibition. We concluded that α-NF promotes pro-collagen production and inhibits MMP-1 expression via the activation of a PI3K/Akt/Smad-3 pathway in normal and UVB-irradiated human skin fibroblasts, while TGF-β may play an important role in transducing this pathway. These results suggest that α-NF, a natural plant product, has the potential to become a novel anti-ageing skin application.

Original languageEnglish
Pages (from-to)546-548
Number of pages3
JournalExperimental Dermatology
Volume21
Issue number7
DOIs
Publication statusPublished - Jul 2012
Externally publishedYes

Fingerprint

Fibroblasts
Matrix Metalloproteinases
Skin
Collagen
Aging of materials
Chemical activation
Phosphatidylinositol 3-Kinases
Skin Aging
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Transcription Factor AP-1
Collagen Type I
Esthetics
Proteins
Cells
alpha-naphthoflavone

Keywords

  • Alpha-naphthoflavone
  • PI3K/Akt/Smad-3
  • Procollagen
  • UVB-irradiated

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

Cite this

Anti-ageing effects of alpha-naphthoflavone on normal and UVB-irradiated human skin fibroblasts. / Liao, Po Lin; Li, Ching Hao; Chang, Cheng Yi; Lu, Siang Rong; Lin, Cheng Hui; Tse, Ling Shan; Cheng, Yu Wen.

In: Experimental Dermatology, Vol. 21, No. 7, 07.2012, p. 546-548.

Research output: Contribution to journalArticle

Liao, Po Lin ; Li, Ching Hao ; Chang, Cheng Yi ; Lu, Siang Rong ; Lin, Cheng Hui ; Tse, Ling Shan ; Cheng, Yu Wen. / Anti-ageing effects of alpha-naphthoflavone on normal and UVB-irradiated human skin fibroblasts. In: Experimental Dermatology. 2012 ; Vol. 21, No. 7. pp. 546-548.
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