Anti-α-glucosidase and Anti-dipeptidyl Peptidase-IV Activities of Extracts and Purified Compounds from Vitis thunbergii var. taiwaniana

Yin Shiou Lin, Chiy Rong Chen, Wei Hau Wu, Chi Luan Wen, Chi I. Chang, Wen Chi Hou

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Ethanol extracts (Et) from the stem (S) and leaf (L) of Vitis thunbergii var. taiwaniana (VTT) were used to investigate yeast α-glucosidase and porcine kidney dipeptidyl peptidase-IV (DPP-IV) inhibitory activities. Both VTT-Et showed complete α-glucosidase inhibition at 0.1 mg/mL; VTT-S-Et and VTT-L-Et showed 26 and 11% DPP-IV inhibition, respectively, at 0.5 mg/mL. The VTT-Et interventions (20 and 50 mg/kg) resulted in improvements in impaired glucose tolerance of diet-induced obese rats. (+)-Hopeaphenol, (+)-vitisin A, and (-)-vitisin B were isolated from the ethyl acetate fractions of S-Et and showed yeast α-glucosidase inhibition (IC50 = 18.30, 1.22, and 1.02 μM) and porcine kidney DPP-IV inhibition (IC50 = 401, 90.75, and 15.3 μM) compared to acarbose (6.39 mM) and sitagliptin (47.35 nM), respectively. Both (+)-vitisin A and (-)-vitisin B showed mixed noncompetitive inhibition against yeast α-glucosidase and porcine kidney DPP-IV, respectively. These results proposed that VTT extracts might through inhibitions against α-glucosidase and DPP-IV improve the impaired glucose tolerance in diet-induced obese rats.

Original languageEnglish
Pages (from-to)6393-6401
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number28
DOIs
Publication statusPublished - Jul 22 2015

Fingerprint

Glucosidases
Dipeptidyl Peptidase 4
glucosidases
Vitis
Yeast
extracts
Glucose Intolerance
Swine
Yeasts
Nutrition
Kidney
Inhibitory Concentration 50
Rats
kidneys
glucose tolerance
yeasts
Acarbose
Diet
swine
Glucose

Keywords

  • (+)-vitisin A
  • (-)-vitisin B
  • blood sugar
  • dipeptidyl peptidase-IV (DPP-IV)
  • impaired glucose tolerance
  • Vitis thunbergii var. taiwaniana (VTT)
  • α-glucosidase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Anti-α-glucosidase and Anti-dipeptidyl Peptidase-IV Activities of Extracts and Purified Compounds from Vitis thunbergii var. taiwaniana. / Lin, Yin Shiou; Chen, Chiy Rong; Wu, Wei Hau; Wen, Chi Luan; Chang, Chi I.; Hou, Wen Chi.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 28, 22.07.2015, p. 6393-6401.

Research output: Contribution to journalArticle

@article{204bfc7515334e6b8f9da3e2b48b0c80,
title = "Anti-α-glucosidase and Anti-dipeptidyl Peptidase-IV Activities of Extracts and Purified Compounds from Vitis thunbergii var. taiwaniana",
abstract = "Ethanol extracts (Et) from the stem (S) and leaf (L) of Vitis thunbergii var. taiwaniana (VTT) were used to investigate yeast α-glucosidase and porcine kidney dipeptidyl peptidase-IV (DPP-IV) inhibitory activities. Both VTT-Et showed complete α-glucosidase inhibition at 0.1 mg/mL; VTT-S-Et and VTT-L-Et showed 26 and 11{\%} DPP-IV inhibition, respectively, at 0.5 mg/mL. The VTT-Et interventions (20 and 50 mg/kg) resulted in improvements in impaired glucose tolerance of diet-induced obese rats. (+)-Hopeaphenol, (+)-vitisin A, and (-)-vitisin B were isolated from the ethyl acetate fractions of S-Et and showed yeast α-glucosidase inhibition (IC50 = 18.30, 1.22, and 1.02 μM) and porcine kidney DPP-IV inhibition (IC50 = 401, 90.75, and 15.3 μM) compared to acarbose (6.39 mM) and sitagliptin (47.35 nM), respectively. Both (+)-vitisin A and (-)-vitisin B showed mixed noncompetitive inhibition against yeast α-glucosidase and porcine kidney DPP-IV, respectively. These results proposed that VTT extracts might through inhibitions against α-glucosidase and DPP-IV improve the impaired glucose tolerance in diet-induced obese rats.",
keywords = "(+)-vitisin A, (-)-vitisin B, blood sugar, dipeptidyl peptidase-IV (DPP-IV), impaired glucose tolerance, Vitis thunbergii var. taiwaniana (VTT), α-glucosidase",
author = "Lin, {Yin Shiou} and Chen, {Chiy Rong} and Wu, {Wei Hau} and Wen, {Chi Luan} and Chang, {Chi I.} and Hou, {Wen Chi}",
year = "2015",
month = "7",
day = "22",
doi = "10.1021/acs.jafc.5b02069",
language = "English",
volume = "63",
pages = "6393--6401",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "28",

}

TY - JOUR

T1 - Anti-α-glucosidase and Anti-dipeptidyl Peptidase-IV Activities of Extracts and Purified Compounds from Vitis thunbergii var. taiwaniana

AU - Lin, Yin Shiou

AU - Chen, Chiy Rong

AU - Wu, Wei Hau

AU - Wen, Chi Luan

AU - Chang, Chi I.

AU - Hou, Wen Chi

PY - 2015/7/22

Y1 - 2015/7/22

N2 - Ethanol extracts (Et) from the stem (S) and leaf (L) of Vitis thunbergii var. taiwaniana (VTT) were used to investigate yeast α-glucosidase and porcine kidney dipeptidyl peptidase-IV (DPP-IV) inhibitory activities. Both VTT-Et showed complete α-glucosidase inhibition at 0.1 mg/mL; VTT-S-Et and VTT-L-Et showed 26 and 11% DPP-IV inhibition, respectively, at 0.5 mg/mL. The VTT-Et interventions (20 and 50 mg/kg) resulted in improvements in impaired glucose tolerance of diet-induced obese rats. (+)-Hopeaphenol, (+)-vitisin A, and (-)-vitisin B were isolated from the ethyl acetate fractions of S-Et and showed yeast α-glucosidase inhibition (IC50 = 18.30, 1.22, and 1.02 μM) and porcine kidney DPP-IV inhibition (IC50 = 401, 90.75, and 15.3 μM) compared to acarbose (6.39 mM) and sitagliptin (47.35 nM), respectively. Both (+)-vitisin A and (-)-vitisin B showed mixed noncompetitive inhibition against yeast α-glucosidase and porcine kidney DPP-IV, respectively. These results proposed that VTT extracts might through inhibitions against α-glucosidase and DPP-IV improve the impaired glucose tolerance in diet-induced obese rats.

AB - Ethanol extracts (Et) from the stem (S) and leaf (L) of Vitis thunbergii var. taiwaniana (VTT) were used to investigate yeast α-glucosidase and porcine kidney dipeptidyl peptidase-IV (DPP-IV) inhibitory activities. Both VTT-Et showed complete α-glucosidase inhibition at 0.1 mg/mL; VTT-S-Et and VTT-L-Et showed 26 and 11% DPP-IV inhibition, respectively, at 0.5 mg/mL. The VTT-Et interventions (20 and 50 mg/kg) resulted in improvements in impaired glucose tolerance of diet-induced obese rats. (+)-Hopeaphenol, (+)-vitisin A, and (-)-vitisin B were isolated from the ethyl acetate fractions of S-Et and showed yeast α-glucosidase inhibition (IC50 = 18.30, 1.22, and 1.02 μM) and porcine kidney DPP-IV inhibition (IC50 = 401, 90.75, and 15.3 μM) compared to acarbose (6.39 mM) and sitagliptin (47.35 nM), respectively. Both (+)-vitisin A and (-)-vitisin B showed mixed noncompetitive inhibition against yeast α-glucosidase and porcine kidney DPP-IV, respectively. These results proposed that VTT extracts might through inhibitions against α-glucosidase and DPP-IV improve the impaired glucose tolerance in diet-induced obese rats.

KW - (+)-vitisin A

KW - (-)-vitisin B

KW - blood sugar

KW - dipeptidyl peptidase-IV (DPP-IV)

KW - impaired glucose tolerance

KW - Vitis thunbergii var. taiwaniana (VTT)

KW - α-glucosidase

UR - http://www.scopus.com/inward/record.url?scp=84937721348&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937721348&partnerID=8YFLogxK

U2 - 10.1021/acs.jafc.5b02069

DO - 10.1021/acs.jafc.5b02069

M3 - Article

C2 - 26138774

AN - SCOPUS:84937721348

VL - 63

SP - 6393

EP - 6401

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 28

ER -