Anti-α-glucosidase and Anti-dipeptidyl Peptidase-IV Activities of Extracts and Purified Compounds from Vitis thunbergii var. taiwaniana

Yin Shiou Lin, Chiy Rong Chen, Wei Hau Wu, Chi Luan Wen, Chi I. Chang, Wen Chi Hou

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Ethanol extracts (Et) from the stem (S) and leaf (L) of Vitis thunbergii var. taiwaniana (VTT) were used to investigate yeast α-glucosidase and porcine kidney dipeptidyl peptidase-IV (DPP-IV) inhibitory activities. Both VTT-Et showed complete α-glucosidase inhibition at 0.1 mg/mL; VTT-S-Et and VTT-L-Et showed 26 and 11% DPP-IV inhibition, respectively, at 0.5 mg/mL. The VTT-Et interventions (20 and 50 mg/kg) resulted in improvements in impaired glucose tolerance of diet-induced obese rats. (+)-Hopeaphenol, (+)-vitisin A, and (-)-vitisin B were isolated from the ethyl acetate fractions of S-Et and showed yeast α-glucosidase inhibition (IC50 = 18.30, 1.22, and 1.02 μM) and porcine kidney DPP-IV inhibition (IC50 = 401, 90.75, and 15.3 μM) compared to acarbose (6.39 mM) and sitagliptin (47.35 nM), respectively. Both (+)-vitisin A and (-)-vitisin B showed mixed noncompetitive inhibition against yeast α-glucosidase and porcine kidney DPP-IV, respectively. These results proposed that VTT extracts might through inhibitions against α-glucosidase and DPP-IV improve the impaired glucose tolerance in diet-induced obese rats.

Original languageEnglish
Pages (from-to)6393-6401
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number28
DOIs
Publication statusPublished - Jul 22 2015

Keywords

  • (+)-vitisin A
  • (-)-vitisin B
  • blood sugar
  • dipeptidyl peptidase-IV (DPP-IV)
  • impaired glucose tolerance
  • Vitis thunbergii var. taiwaniana (VTT)
  • α-glucosidase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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