Annexin-I as a potential target for green tea extract induced actin remodeling

Gui Shan Xiao, Yu Sheng Jin, Qing Yi Lu, Zuo Feng Zhang, Arie Belldegrun, Robert Figlin, Allan Pantuck, Yun Yen, Frederick Li, Jian Yu Rao

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Using a multistep human urothelial model, we previously showed that green tea extract (GTE) selectively modulates actin remodeling in transformed cells (MC-T11), which resulted in increased cell adhesion and reduced cell motility (Lu et al., Clin Cancer Res 2005;11:1675-83). This study further analyzed which actin binding proteins (ABPs) might be involved in this process. Proteomic profiles of GTE treated and untreated MC-T11 cells using two-dimensional gel electrophoresis coupled with liquid chromatography tandem mass spectrometry (LC/MS/MS) and matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) identified 20 GTE-induced proteins. Among them, 3 were ABPs (tropomodulin, cofilin and annexin-I), and only annexin-I showed a dose- and time-dependent expression. The increased annexin-I correlated with actin remodeling, and was the result of transcription level up-regulation, as determined by RT-PCR, pull-down immunoblot and siRNA analyses. 5-Azacytidine, a DNA methylation inhibitor, exhibited no effect on annexin-I expression when used alone, but had an additive effect for GTE-induced annexin-I expression. Immunohistochemistry of bladder cancer tissue array showed a decrease of annexin-I expression in carcinoma in situ and low grade papillary carcinoma (n = 32, 0% positive) compared to nontumor urothelium (n = 18, 89% positive) (p < 0.001 by Fisher exact test), but increased in some (6 of 15, 40%) high-grade tumors. Together, GTE induced annexin-I expression plays a role in regulating actin remodeling and decreased annexin-I expression is a common event in early stage of bladder cancer development.

Original languageEnglish
Pages (from-to)111-120
Number of pages10
JournalInternational Journal of Cancer
Volume120
Issue number1
DOIs
Publication statusPublished - Jan 1 2007
Externally publishedYes

Fingerprint

Annexin A1
Tea
Actins
Microfilament Proteins
Urinary Bladder Neoplasms
Tropomodulin
Actin Depolymerizing Factors
Azacitidine
Urothelium
Papillary Carcinoma
Carcinoma in Situ
Electrophoresis, Gel, Two-Dimensional
DNA Methylation
Tandem Mass Spectrometry
Cell Adhesion
Liquid Chromatography
Proteomics
Small Interfering RNA
Cell Movement
Neoplasms

Keywords

  • Actin remodeling
  • Annexin-I
  • Bladder cancer
  • Chemoprevention
  • Green tea extract
  • Proteomics

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Xiao, G. S., Jin, Y. S., Lu, Q. Y., Zhang, Z. F., Belldegrun, A., Figlin, R., ... Rao, J. Y. (2007). Annexin-I as a potential target for green tea extract induced actin remodeling. International Journal of Cancer, 120(1), 111-120. https://doi.org/10.1002/ijc.22164

Annexin-I as a potential target for green tea extract induced actin remodeling. / Xiao, Gui Shan; Jin, Yu Sheng; Lu, Qing Yi; Zhang, Zuo Feng; Belldegrun, Arie; Figlin, Robert; Pantuck, Allan; Yen, Yun; Li, Frederick; Rao, Jian Yu.

In: International Journal of Cancer, Vol. 120, No. 1, 01.01.2007, p. 111-120.

Research output: Contribution to journalArticle

Xiao, GS, Jin, YS, Lu, QY, Zhang, ZF, Belldegrun, A, Figlin, R, Pantuck, A, Yen, Y, Li, F & Rao, JY 2007, 'Annexin-I as a potential target for green tea extract induced actin remodeling', International Journal of Cancer, vol. 120, no. 1, pp. 111-120. https://doi.org/10.1002/ijc.22164
Xiao GS, Jin YS, Lu QY, Zhang ZF, Belldegrun A, Figlin R et al. Annexin-I as a potential target for green tea extract induced actin remodeling. International Journal of Cancer. 2007 Jan 1;120(1):111-120. https://doi.org/10.1002/ijc.22164
Xiao, Gui Shan ; Jin, Yu Sheng ; Lu, Qing Yi ; Zhang, Zuo Feng ; Belldegrun, Arie ; Figlin, Robert ; Pantuck, Allan ; Yen, Yun ; Li, Frederick ; Rao, Jian Yu. / Annexin-I as a potential target for green tea extract induced actin remodeling. In: International Journal of Cancer. 2007 ; Vol. 120, No. 1. pp. 111-120.
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