Angiopoietins modulate endothelial adaptation, glomerular and podocyte hypertrophy after uninephrectomy

Wen Chih Chiang, Chun Fu Lai, Chi Ting Su, Wei Hao Peng, Ching Fang Wu, Fan Chi Chang, Yi Ting Chen, Shuei Liong Lin, Yung Ming Chen, Kwan Dun Wu, Kuo Shyan Lu, Tun Jun Tsai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Glomerular capillary remodeling is an essential process in the development of glomerular hypertrophy. Angiopoietins, which are important regulators in angiogenesis, plays a role in the development of glomerulus during embryogenesis. Here, we evaluated the influence of angiopoietin on glomerular components and hypertrophy after uninephrectomy in adult male BALB/c mice. The actions of angiopoietin 1 or 2 were systemically antagonized by the subcutaneous administration of antagonists. We observed that the angiopoietin system was activated after uninephrectomy, and that the blockade of angiopoietin 1 or 2 decreased the activation of the angiopoietin receptor - tyrosine kinase with Ig and EGF homology domains-2 - and attenuated the development of glomerular and podocyte hypertrophy. The increase in endothelial density staining (anti-CD31) following uninephrectomy was also reversed by angiopoietin 1 or 2 blockades. Glomerular basement thickness and foot process width were observed to decrease in the angiopoietin blockade groups. These changes were associated with the down regulation of the expression of genes for the glomerular matrix and basement membrane, including collagen type IV α1, collagen type IV α2, collagen type IV α5, and laminin α5. Thus, angiopoietin 1 or 2 may play an important role in the development of glomerular hypertrophy after uninephrectomy. A blockade of the angiopoietin system not only influenced the endothelium but also the podocyte, leading to diminished gene expression and morphological changes after uninephrectomy.

Original languageEnglish
Article numbere82592
JournalPLoS One
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 18 2013
Externally publishedYes

Fingerprint

Angiopoietins
Podocytes
hypertrophy
Angiopoietin-2
Angiopoietin-1
Hypertrophy
Collagen Type IV
collagen
Collagen Type II
Collagen Type V
Gene Expression
Glomerular Basement Membrane
laminin
basement membrane
gene expression regulation
Receptor Protein-Tyrosine Kinases
subcutaneous injection
Collagen Type I
angiogenesis
endothelium

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chiang, W. C., Lai, C. F., Su, C. T., Peng, W. H., Wu, C. F., Chang, F. C., ... Tsai, T. J. (2013). Angiopoietins modulate endothelial adaptation, glomerular and podocyte hypertrophy after uninephrectomy. PLoS One, 8(12), [e82592]. https://doi.org/10.1371/journal.pone.0082592

Angiopoietins modulate endothelial adaptation, glomerular and podocyte hypertrophy after uninephrectomy. / Chiang, Wen Chih; Lai, Chun Fu; Su, Chi Ting; Peng, Wei Hao; Wu, Ching Fang; Chang, Fan Chi; Chen, Yi Ting; Lin, Shuei Liong; Chen, Yung Ming; Wu, Kwan Dun; Lu, Kuo Shyan; Tsai, Tun Jun.

In: PLoS One, Vol. 8, No. 12, e82592, 18.12.2013.

Research output: Contribution to journalArticle

Chiang, WC, Lai, CF, Su, CT, Peng, WH, Wu, CF, Chang, FC, Chen, YT, Lin, SL, Chen, YM, Wu, KD, Lu, KS & Tsai, TJ 2013, 'Angiopoietins modulate endothelial adaptation, glomerular and podocyte hypertrophy after uninephrectomy', PLoS One, vol. 8, no. 12, e82592. https://doi.org/10.1371/journal.pone.0082592
Chiang, Wen Chih ; Lai, Chun Fu ; Su, Chi Ting ; Peng, Wei Hao ; Wu, Ching Fang ; Chang, Fan Chi ; Chen, Yi Ting ; Lin, Shuei Liong ; Chen, Yung Ming ; Wu, Kwan Dun ; Lu, Kuo Shyan ; Tsai, Tun Jun. / Angiopoietins modulate endothelial adaptation, glomerular and podocyte hypertrophy after uninephrectomy. In: PLoS One. 2013 ; Vol. 8, No. 12.
@article{d8035d9f4c024786a1c112cd98ee1c27,
title = "Angiopoietins modulate endothelial adaptation, glomerular and podocyte hypertrophy after uninephrectomy",
abstract = "Glomerular capillary remodeling is an essential process in the development of glomerular hypertrophy. Angiopoietins, which are important regulators in angiogenesis, plays a role in the development of glomerulus during embryogenesis. Here, we evaluated the influence of angiopoietin on glomerular components and hypertrophy after uninephrectomy in adult male BALB/c mice. The actions of angiopoietin 1 or 2 were systemically antagonized by the subcutaneous administration of antagonists. We observed that the angiopoietin system was activated after uninephrectomy, and that the blockade of angiopoietin 1 or 2 decreased the activation of the angiopoietin receptor - tyrosine kinase with Ig and EGF homology domains-2 - and attenuated the development of glomerular and podocyte hypertrophy. The increase in endothelial density staining (anti-CD31) following uninephrectomy was also reversed by angiopoietin 1 or 2 blockades. Glomerular basement thickness and foot process width were observed to decrease in the angiopoietin blockade groups. These changes were associated with the down regulation of the expression of genes for the glomerular matrix and basement membrane, including collagen type IV α1, collagen type IV α2, collagen type IV α5, and laminin α5. Thus, angiopoietin 1 or 2 may play an important role in the development of glomerular hypertrophy after uninephrectomy. A blockade of the angiopoietin system not only influenced the endothelium but also the podocyte, leading to diminished gene expression and morphological changes after uninephrectomy.",
author = "Chiang, {Wen Chih} and Lai, {Chun Fu} and Su, {Chi Ting} and Peng, {Wei Hao} and Wu, {Ching Fang} and Chang, {Fan Chi} and Chen, {Yi Ting} and Lin, {Shuei Liong} and Chen, {Yung Ming} and Wu, {Kwan Dun} and Lu, {Kuo Shyan} and Tsai, {Tun Jun}",
year = "2013",
month = "12",
day = "18",
doi = "10.1371/journal.pone.0082592",
language = "English",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - Angiopoietins modulate endothelial adaptation, glomerular and podocyte hypertrophy after uninephrectomy

AU - Chiang, Wen Chih

AU - Lai, Chun Fu

AU - Su, Chi Ting

AU - Peng, Wei Hao

AU - Wu, Ching Fang

AU - Chang, Fan Chi

AU - Chen, Yi Ting

AU - Lin, Shuei Liong

AU - Chen, Yung Ming

AU - Wu, Kwan Dun

AU - Lu, Kuo Shyan

AU - Tsai, Tun Jun

PY - 2013/12/18

Y1 - 2013/12/18

N2 - Glomerular capillary remodeling is an essential process in the development of glomerular hypertrophy. Angiopoietins, which are important regulators in angiogenesis, plays a role in the development of glomerulus during embryogenesis. Here, we evaluated the influence of angiopoietin on glomerular components and hypertrophy after uninephrectomy in adult male BALB/c mice. The actions of angiopoietin 1 or 2 were systemically antagonized by the subcutaneous administration of antagonists. We observed that the angiopoietin system was activated after uninephrectomy, and that the blockade of angiopoietin 1 or 2 decreased the activation of the angiopoietin receptor - tyrosine kinase with Ig and EGF homology domains-2 - and attenuated the development of glomerular and podocyte hypertrophy. The increase in endothelial density staining (anti-CD31) following uninephrectomy was also reversed by angiopoietin 1 or 2 blockades. Glomerular basement thickness and foot process width were observed to decrease in the angiopoietin blockade groups. These changes were associated with the down regulation of the expression of genes for the glomerular matrix and basement membrane, including collagen type IV α1, collagen type IV α2, collagen type IV α5, and laminin α5. Thus, angiopoietin 1 or 2 may play an important role in the development of glomerular hypertrophy after uninephrectomy. A blockade of the angiopoietin system not only influenced the endothelium but also the podocyte, leading to diminished gene expression and morphological changes after uninephrectomy.

AB - Glomerular capillary remodeling is an essential process in the development of glomerular hypertrophy. Angiopoietins, which are important regulators in angiogenesis, plays a role in the development of glomerulus during embryogenesis. Here, we evaluated the influence of angiopoietin on glomerular components and hypertrophy after uninephrectomy in adult male BALB/c mice. The actions of angiopoietin 1 or 2 were systemically antagonized by the subcutaneous administration of antagonists. We observed that the angiopoietin system was activated after uninephrectomy, and that the blockade of angiopoietin 1 or 2 decreased the activation of the angiopoietin receptor - tyrosine kinase with Ig and EGF homology domains-2 - and attenuated the development of glomerular and podocyte hypertrophy. The increase in endothelial density staining (anti-CD31) following uninephrectomy was also reversed by angiopoietin 1 or 2 blockades. Glomerular basement thickness and foot process width were observed to decrease in the angiopoietin blockade groups. These changes were associated with the down regulation of the expression of genes for the glomerular matrix and basement membrane, including collagen type IV α1, collagen type IV α2, collagen type IV α5, and laminin α5. Thus, angiopoietin 1 or 2 may play an important role in the development of glomerular hypertrophy after uninephrectomy. A blockade of the angiopoietin system not only influenced the endothelium but also the podocyte, leading to diminished gene expression and morphological changes after uninephrectomy.

UR - http://www.scopus.com/inward/record.url?scp=84893181875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893181875&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0082592

DO - 10.1371/journal.pone.0082592

M3 - Article

C2 - 24367525

AN - SCOPUS:84893181875

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e82592

ER -