Angiogenesis Inhibitors and Anti-Inflammatory Agents from Phoma sp. NTOU4195

Ming Shian Lee, Shih Wei Wang, Guei Jane Wang, Ka Lai Pang, Ching Kuo Lee, Yueh Hsiung Kuo, Hyo Jung Cha, Ruo Kai Lin, Tzong Huei Lee

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19 Citations (Scopus)

Abstract

Seven new polyketides, phomaketides A-E (1-5) and pseurotins A3 (6) and G (7), along with the known compounds FR-111142, pseurotins A, A1, A2, D, and F2, 14-norpseurotin A, α-carbonylcarbene, tyrosol, cyclo(-l-Pro-l-Leu), and cyclo(-l-Pro-l-Phe), were purified from the fermentation broth and mycelium of the endophytic fungal strain Phoma sp. NTOU4195 isolated from the marine red alga Pterocladiella capillacea. The structures were established through interpretation of spectroscopic data. The antiangiogenic and anti-inflammatory effects of 1-7 and related analogues were evaluated using human endothelial progenitor cells (EPCs) and lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells, respectively. Of the compounds tested, compound 1 exhibited the most potent antiangiogenic activity by suppressing the tube formation of EPCs with an IC50 of 8.1 μM, and compound 3 showed the most selective inhibitory activity of LPS-induced NO production in RAW264.7 macrophages with an IC50 value of 8.8 μM.

Original languageEnglish
Pages (from-to)2983-2990
Number of pages8
JournalJournal of Natural Products
Volume79
Issue number12
DOIs
Publication statusPublished - Dec 23 2016

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this

Lee, M. S., Wang, S. W., Wang, G. J., Pang, K. L., Lee, C. K., Kuo, Y. H., Cha, H. J., Lin, R. K., & Lee, T. H. (2016). Angiogenesis Inhibitors and Anti-Inflammatory Agents from Phoma sp. NTOU4195. Journal of Natural Products, 79(12), 2983-2990. https://doi.org/10.1021/acs.jnatprod.6b00407