Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway

Tzu Shao Yeh, Cheng-Chen Hsu, Suh-Ching Yang, Mei Chich Hsu, Jen-Fang Liu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Angelica Sinensis (AS), a folk medicine, has long been used in ergogenic aids for athletes, but there is little scientific evidence supporting its effects. We investigated whether AS induces hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/mammalian target of the rapamycin (mTOR) pathway. Methods: An in vitro experiment investigating the induction of hypertrophy in myotubes was conducted. To investigate whether AS promoted the hypertrophy of myotubes, an established in vitro model of myotube hypertrophy with and without AS was used and examined using microscopic images. The role of the PI3K/Akt/mTOR signaling pathway in AS-induced myotube hypertrophy was evaluated. Two inhibitors, wortmannin (an inhibitor of PI3K) and rapamycin (an inhibitor of mTOR), were used. Result: The results revealed that the myotube diameters in the AS-treated group were significantly larger than those in the untreated control group (P <0.05). Wortmannin and rapamycin inhibited AS-induced hypertrophy. Furthermore, AS increased Akt and mTOR phosphorylation through the PI3K pathway and induced myotube hypertrophy. Conclusion: The results confirmed that AS induces hypertrophy in myotubes through the PI3K/Akt/mTOR pathway.

Original languageEnglish
Article number144
JournalBMC Complementary and Alternative Medicine
Volume14
Issue number1
DOIs
Publication statusPublished - May 3 2014

Fingerprint

Angelica sinensis
Phosphatidylinositol 3-Kinase
Skeletal Muscle Fibers
Sirolimus
Hypertrophy
Traditional Medicine
Athletes
Phosphorylation

Keywords

  • C2C12
  • Dong Quai
  • IGF-1
  • Muscle

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway. / Yeh, Tzu Shao; Hsu, Cheng-Chen; Yang, Suh-Ching; Hsu, Mei Chich; Liu, Jen-Fang.

In: BMC Complementary and Alternative Medicine, Vol. 14, No. 1, 144, 03.05.2014.

Research output: Contribution to journalArticle

@article{de11486f4ce74e3c98bdd6af45372276,
title = "Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway",
abstract = "Background: Angelica Sinensis (AS), a folk medicine, has long been used in ergogenic aids for athletes, but there is little scientific evidence supporting its effects. We investigated whether AS induces hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/mammalian target of the rapamycin (mTOR) pathway. Methods: An in vitro experiment investigating the induction of hypertrophy in myotubes was conducted. To investigate whether AS promoted the hypertrophy of myotubes, an established in vitro model of myotube hypertrophy with and without AS was used and examined using microscopic images. The role of the PI3K/Akt/mTOR signaling pathway in AS-induced myotube hypertrophy was evaluated. Two inhibitors, wortmannin (an inhibitor of PI3K) and rapamycin (an inhibitor of mTOR), were used. Result: The results revealed that the myotube diameters in the AS-treated group were significantly larger than those in the untreated control group (P <0.05). Wortmannin and rapamycin inhibited AS-induced hypertrophy. Furthermore, AS increased Akt and mTOR phosphorylation through the PI3K pathway and induced myotube hypertrophy. Conclusion: The results confirmed that AS induces hypertrophy in myotubes through the PI3K/Akt/mTOR pathway.",
keywords = "C2C12, Dong Quai, IGF-1, Muscle",
author = "Yeh, {Tzu Shao} and Cheng-Chen Hsu and Suh-Ching Yang and Hsu, {Mei Chich} and Jen-Fang Liu",
year = "2014",
month = "5",
day = "3",
doi = "10.1186/1472-6882-14-144",
language = "English",
volume = "14",
journal = "BMC Complementary and Alternative Medicine",
issn = "1472-6882",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway

AU - Yeh, Tzu Shao

AU - Hsu, Cheng-Chen

AU - Yang, Suh-Ching

AU - Hsu, Mei Chich

AU - Liu, Jen-Fang

PY - 2014/5/3

Y1 - 2014/5/3

N2 - Background: Angelica Sinensis (AS), a folk medicine, has long been used in ergogenic aids for athletes, but there is little scientific evidence supporting its effects. We investigated whether AS induces hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/mammalian target of the rapamycin (mTOR) pathway. Methods: An in vitro experiment investigating the induction of hypertrophy in myotubes was conducted. To investigate whether AS promoted the hypertrophy of myotubes, an established in vitro model of myotube hypertrophy with and without AS was used and examined using microscopic images. The role of the PI3K/Akt/mTOR signaling pathway in AS-induced myotube hypertrophy was evaluated. Two inhibitors, wortmannin (an inhibitor of PI3K) and rapamycin (an inhibitor of mTOR), were used. Result: The results revealed that the myotube diameters in the AS-treated group were significantly larger than those in the untreated control group (P <0.05). Wortmannin and rapamycin inhibited AS-induced hypertrophy. Furthermore, AS increased Akt and mTOR phosphorylation through the PI3K pathway and induced myotube hypertrophy. Conclusion: The results confirmed that AS induces hypertrophy in myotubes through the PI3K/Akt/mTOR pathway.

AB - Background: Angelica Sinensis (AS), a folk medicine, has long been used in ergogenic aids for athletes, but there is little scientific evidence supporting its effects. We investigated whether AS induces hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/mammalian target of the rapamycin (mTOR) pathway. Methods: An in vitro experiment investigating the induction of hypertrophy in myotubes was conducted. To investigate whether AS promoted the hypertrophy of myotubes, an established in vitro model of myotube hypertrophy with and without AS was used and examined using microscopic images. The role of the PI3K/Akt/mTOR signaling pathway in AS-induced myotube hypertrophy was evaluated. Two inhibitors, wortmannin (an inhibitor of PI3K) and rapamycin (an inhibitor of mTOR), were used. Result: The results revealed that the myotube diameters in the AS-treated group were significantly larger than those in the untreated control group (P <0.05). Wortmannin and rapamycin inhibited AS-induced hypertrophy. Furthermore, AS increased Akt and mTOR phosphorylation through the PI3K pathway and induced myotube hypertrophy. Conclusion: The results confirmed that AS induces hypertrophy in myotubes through the PI3K/Akt/mTOR pathway.

KW - C2C12

KW - Dong Quai

KW - IGF-1

KW - Muscle

UR - http://www.scopus.com/inward/record.url?scp=84938591840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938591840&partnerID=8YFLogxK

U2 - 10.1186/1472-6882-14-144

DO - 10.1186/1472-6882-14-144

M3 - Article

VL - 14

JO - BMC Complementary and Alternative Medicine

JF - BMC Complementary and Alternative Medicine

SN - 1472-6882

IS - 1

M1 - 144

ER -