Anesthetics and human epidermal growth factor incorporated into anti-adhesive nanofibers provide sustained pain relief and promote healing of surgical wounds

Shih Jung Liu, Ching Wei Kao, Yuan Yun Tseng, Kuo Sheng Liu, Yen Wei Liu, Jin Chung Chen, Hong Lin He, Yi Chuan Kau

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: This study exploited sheath-core-structured lidocaine/human EGF (hEGF)- loaded anti-adhesive poly[(d,l)-lactide-co-glycolide] (PLGA) nanofibrous films for surgical wounds via a co-axial electrospinning technique. Materials and methods: After spinning, the properties of the co-axially spun membranes were characterized by scanning electron microscopy, laser-scanning confocal microscopy, Fourier Transform Infrared spectrometry, water contact angle measurements, and tensile tests. Furthermore, a HPLC analysis and an ELISA evaluated the in vitro and in vivo release curves of lidocaine and hEGF from the films. Results: PLGA anti-adhesion nanofibers eluted high levels of lidocaine and hEGF for over 32 and 27 days, respectively, in vitro. The in vivo evaluation of post-surgery recovery in a rat model demonstrated that no adhesion was noticed in tissues at 2 weeks after surgery illustrating the anti-adhesive performance of the sheath-core-structured nanofibers. Nanofibrous films effectively released lidocaine and hEGF for >2 weeks in vivo. In addition, rats implanted with the lidocaine/hEGF nanofibrous membranes exhibited greater activities than the control demonstrating the pain relief efficacy of the films. Conclusion: The empirical outcomes suggested that the anti-adhesive nanofibrous films with extended release of lidocaine and hEGF offer post-operative pain relief and wound healing.

Original languageEnglish
Pages (from-to)4007-4016
Number of pages10
JournalInternational Journal of Nanomedicine
Volume14
DOIs
Publication statusPublished - Jan 1 2019

Keywords

  • Anti-adhesive membrane
  • HEGF
  • Lidocaine
  • Sheath-core nanofiber
  • Sustained elution

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

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