Andrology: Co-culture with vero cell monolayer maintains the motility of asthenozoospermic semen samples

Hsin Fu Chen, Hong Nerng Ho, Shee Uan Chen, Yih Ron Lien, Kuang Han Chao, Heng Ru Lin, Su Cheng Huang, Tzu Yao Lee, Yu Shih Yang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The clinical effectiveness of co-culture with Vero (Green monkey kidney) cell monolayer in maintaining the motility and viability of fresh asthenozoospermic semen (18 samples) and frozen-thawed semen with poor motility (motility fraction <50%) (15 samples) in a 24-h period was evaluated. Co-culture with Vero cell monolayer in human tubal fluid (HTF) medium for 24 h resulted in a statistically better maintenance of motility percentage (P < 0.005), mean amplitude of lateral head displacement (ALH) (P < 0.005), and mean track speed (VCL) (P < 0.05) than culture in HTF medium alone. However, these motility parameters (motility percentage, ALH, VCL) declined soon after removal of spermatozoa from the monolayer. Co-culture with Vero cell monolayer also maintained the viability percentage of these sperm samples (52% of the original value) after the 24-h period compared with culture in HTF medium alone (22% of the original) (52% versus 22%, P < 0.05).It is concluded that Vero cell monolayer is effective in the maintenance of motility and viability of asthenozoospermic semen or frozen-thawed semen with poor motility.This co-culture system may be beneficial in enhancing the in-vitro performance of asthenozoospermic semen samples in the practice of assisted reproductive technology.However, its safety needs further evaluation.

Original languageEnglish
Pages (from-to)1276-1280
Number of pages5
JournalHuman Reproduction
Volume9
Issue number7
DOIs
Publication statusPublished - Jan 1 1994
Externally publishedYes

Fingerprint

Cytological Techniques
Andrology
Reproductive Techniques
Cercopithecus aethiops
Vero Cells
Sperm Motility
Male Infertility
Cryopreservation
Coculture Techniques
Semen
Culture Media
Cell Survival
Semen Preservation
Spermatozoa
Maintenance
Assisted Reproductive Techniques
Kidney
Safety

Keywords

  • Asthenozoospermic semen
  • Co-culture
  • Computer-aided sperm analysis
  • Vero cell

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Andrology : Co-culture with vero cell monolayer maintains the motility of asthenozoospermic semen samples. / Chen, Hsin Fu; Ho, Hong Nerng; Chen, Shee Uan; Lien, Yih Ron; Chao, Kuang Han; Lin, Heng Ru; Huang, Su Cheng; Lee, Tzu Yao; Yang, Yu Shih.

In: Human Reproduction, Vol. 9, No. 7, 01.01.1994, p. 1276-1280.

Research output: Contribution to journalArticle

Chen, Hsin Fu ; Ho, Hong Nerng ; Chen, Shee Uan ; Lien, Yih Ron ; Chao, Kuang Han ; Lin, Heng Ru ; Huang, Su Cheng ; Lee, Tzu Yao ; Yang, Yu Shih. / Andrology : Co-culture with vero cell monolayer maintains the motility of asthenozoospermic semen samples. In: Human Reproduction. 1994 ; Vol. 9, No. 7. pp. 1276-1280.
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