Androgen-induced human breast cancer cell proliferation is mediated by discrete mechanisms in estrogen receptor-α-positive and -negative breast cancer cells

Hung Yun Lin, Mingzeng Sun, Cassie Lin, Heng Yuan Tang, David London, Ai Shih, Faith B. Davis, Paul J. Davis

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Androgens have important physiological effects in women. Not only are they the precursor hormones for estrogen biosynthesis in the ovaries and extragonadal tissues, but also act directly via androgen receptors (ARs) throughout the body. Studies of the role of androgens on breast cancer development are controversial and the mechanisms involved are not fully understood. In this report we demonstrate that a non-aromatizable androgen metabolite, dihydrotestosterone (DHT), stimulated cell proliferation in vitro of both estrogen receptor-α (ER-α)-positive MCF-7 cells and ER-α-negative MDA-MB-231 human breast cancer cells. A contribution of ER to the proliferative effect of DHT in MCF-7 cells was supported by actions of small interfering RNA (siRNA) ER-α transfection and of the specific inhibitor of ER, ICI 182,780 to block DHT-induced proliferation. A contribution of the possible conversion of DHT to androstane-3α, 17β-diol was not excluded in these MCF-7 cell studies. In MDA-MB-231 cells, a novel mechanism was implicated, in that anti-integrin αvβ3 or an Arg-Gly-Asp (RGD) peptide targeted at a small molecule binding domain of the integrin eliminated the DHT effect on cell proliferation. Anti-integrin αvβ3 did not affect DHT action on MCF-7 cells. A contribution from classical androgen receptor to the DHT effect in each cell line was excluded. A proliferative DHT signal is transduced in both ER-α-positive and ER-α-negative breast cancer cells, but by discrete mechanisms.

Original languageEnglish
Pages (from-to)182-188
Number of pages7
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume113
Issue number3-5
DOIs
Publication statusPublished - Feb 2009
Externally publishedYes

Fingerprint

Dihydrotestosterone
Cell proliferation
Estrogen Receptors
Androgens
Cells
Cell Proliferation
Breast Neoplasms
MCF-7 Cells
Integrins
Androgen Receptors
Androstane-3,17-diol
Biosynthesis
Metabolites
Small Interfering RNA
Transfection
Ovary
Estrogens
Hormones
Tissue
Cell Line

Keywords

  • Androgen
  • Breast cancer
  • Estrogen receptor
  • Integrin αvß3

ASJC Scopus subject areas

  • Molecular Medicine
  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Cell Biology
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry

Cite this

Androgen-induced human breast cancer cell proliferation is mediated by discrete mechanisms in estrogen receptor-α-positive and -negative breast cancer cells. / Lin, Hung Yun; Sun, Mingzeng; Lin, Cassie; Tang, Heng Yuan; London, David; Shih, Ai; Davis, Faith B.; Davis, Paul J.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 113, No. 3-5, 02.2009, p. 182-188.

Research output: Contribution to journalArticle

Lin, Hung Yun ; Sun, Mingzeng ; Lin, Cassie ; Tang, Heng Yuan ; London, David ; Shih, Ai ; Davis, Faith B. ; Davis, Paul J. / Androgen-induced human breast cancer cell proliferation is mediated by discrete mechanisms in estrogen receptor-α-positive and -negative breast cancer cells. In: Journal of Steroid Biochemistry and Molecular Biology. 2009 ; Vol. 113, No. 3-5. pp. 182-188.
@article{89b7f8d57dc94bf3bdf313025fc34cf4,
title = "Androgen-induced human breast cancer cell proliferation is mediated by discrete mechanisms in estrogen receptor-α-positive and -negative breast cancer cells",
abstract = "Androgens have important physiological effects in women. Not only are they the precursor hormones for estrogen biosynthesis in the ovaries and extragonadal tissues, but also act directly via androgen receptors (ARs) throughout the body. Studies of the role of androgens on breast cancer development are controversial and the mechanisms involved are not fully understood. In this report we demonstrate that a non-aromatizable androgen metabolite, dihydrotestosterone (DHT), stimulated cell proliferation in vitro of both estrogen receptor-α (ER-α)-positive MCF-7 cells and ER-α-negative MDA-MB-231 human breast cancer cells. A contribution of ER to the proliferative effect of DHT in MCF-7 cells was supported by actions of small interfering RNA (siRNA) ER-α transfection and of the specific inhibitor of ER, ICI 182,780 to block DHT-induced proliferation. A contribution of the possible conversion of DHT to androstane-3α, 17β-diol was not excluded in these MCF-7 cell studies. In MDA-MB-231 cells, a novel mechanism was implicated, in that anti-integrin αvβ3 or an Arg-Gly-Asp (RGD) peptide targeted at a small molecule binding domain of the integrin eliminated the DHT effect on cell proliferation. Anti-integrin αvβ3 did not affect DHT action on MCF-7 cells. A contribution from classical androgen receptor to the DHT effect in each cell line was excluded. A proliferative DHT signal is transduced in both ER-α-positive and ER-α-negative breast cancer cells, but by discrete mechanisms.",
keywords = "Androgen, Breast cancer, Estrogen receptor, Integrin αv{\ss}3",
author = "Lin, {Hung Yun} and Mingzeng Sun and Cassie Lin and Tang, {Heng Yuan} and David London and Ai Shih and Davis, {Faith B.} and Davis, {Paul J.}",
year = "2009",
month = "2",
doi = "10.1016/j.jsbmb.2008.12.010",
language = "English",
volume = "113",
pages = "182--188",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
issn = "0960-0760",
publisher = "Elsevier Limited",
number = "3-5",

}

TY - JOUR

T1 - Androgen-induced human breast cancer cell proliferation is mediated by discrete mechanisms in estrogen receptor-α-positive and -negative breast cancer cells

AU - Lin, Hung Yun

AU - Sun, Mingzeng

AU - Lin, Cassie

AU - Tang, Heng Yuan

AU - London, David

AU - Shih, Ai

AU - Davis, Faith B.

AU - Davis, Paul J.

PY - 2009/2

Y1 - 2009/2

N2 - Androgens have important physiological effects in women. Not only are they the precursor hormones for estrogen biosynthesis in the ovaries and extragonadal tissues, but also act directly via androgen receptors (ARs) throughout the body. Studies of the role of androgens on breast cancer development are controversial and the mechanisms involved are not fully understood. In this report we demonstrate that a non-aromatizable androgen metabolite, dihydrotestosterone (DHT), stimulated cell proliferation in vitro of both estrogen receptor-α (ER-α)-positive MCF-7 cells and ER-α-negative MDA-MB-231 human breast cancer cells. A contribution of ER to the proliferative effect of DHT in MCF-7 cells was supported by actions of small interfering RNA (siRNA) ER-α transfection and of the specific inhibitor of ER, ICI 182,780 to block DHT-induced proliferation. A contribution of the possible conversion of DHT to androstane-3α, 17β-diol was not excluded in these MCF-7 cell studies. In MDA-MB-231 cells, a novel mechanism was implicated, in that anti-integrin αvβ3 or an Arg-Gly-Asp (RGD) peptide targeted at a small molecule binding domain of the integrin eliminated the DHT effect on cell proliferation. Anti-integrin αvβ3 did not affect DHT action on MCF-7 cells. A contribution from classical androgen receptor to the DHT effect in each cell line was excluded. A proliferative DHT signal is transduced in both ER-α-positive and ER-α-negative breast cancer cells, but by discrete mechanisms.

AB - Androgens have important physiological effects in women. Not only are they the precursor hormones for estrogen biosynthesis in the ovaries and extragonadal tissues, but also act directly via androgen receptors (ARs) throughout the body. Studies of the role of androgens on breast cancer development are controversial and the mechanisms involved are not fully understood. In this report we demonstrate that a non-aromatizable androgen metabolite, dihydrotestosterone (DHT), stimulated cell proliferation in vitro of both estrogen receptor-α (ER-α)-positive MCF-7 cells and ER-α-negative MDA-MB-231 human breast cancer cells. A contribution of ER to the proliferative effect of DHT in MCF-7 cells was supported by actions of small interfering RNA (siRNA) ER-α transfection and of the specific inhibitor of ER, ICI 182,780 to block DHT-induced proliferation. A contribution of the possible conversion of DHT to androstane-3α, 17β-diol was not excluded in these MCF-7 cell studies. In MDA-MB-231 cells, a novel mechanism was implicated, in that anti-integrin αvβ3 or an Arg-Gly-Asp (RGD) peptide targeted at a small molecule binding domain of the integrin eliminated the DHT effect on cell proliferation. Anti-integrin αvβ3 did not affect DHT action on MCF-7 cells. A contribution from classical androgen receptor to the DHT effect in each cell line was excluded. A proliferative DHT signal is transduced in both ER-α-positive and ER-α-negative breast cancer cells, but by discrete mechanisms.

KW - Androgen

KW - Breast cancer

KW - Estrogen receptor

KW - Integrin αvß3

UR - http://www.scopus.com/inward/record.url?scp=61349173908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61349173908&partnerID=8YFLogxK

U2 - 10.1016/j.jsbmb.2008.12.010

DO - 10.1016/j.jsbmb.2008.12.010

M3 - Article

C2 - 19159686

AN - SCOPUS:61349173908

VL - 113

SP - 182

EP - 188

JO - Journal of Steroid Biochemistry and Molecular Biology

JF - Journal of Steroid Biochemistry and Molecular Biology

SN - 0960-0760

IS - 3-5

ER -