Analytical measurement of pegylated molecules

Tian Lu Cheng, Kuo Hsiang Chuang, Bing Mae Chen, Steve R. Roffler

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Attachment of poly(ethylene glycol) (PEG) to proteins, peptides, liposomes, drugs, and nanoparticles can improve pharmaceutical pharmacokinetic properties and enhance in vivo biological efficacy. Since the first PEGylated product was approved by the Food and Drug Administration in 1990, increasing numbers of PEGylated compounds have entered clinical use. Successful clinical development of PEGylated pharmaceuticals requires accurate methods for the qualitative and quantitative analysis of intact PEG conjugates in biological fluids. In this article, we review assay methods that can be utilized for the detection and measurement of PEGylated pharmaceuticals in complex biological samples for determination of biodistribution and pharmacokinetic properties. In particular, we describe relevant colorimetric, chromatographic, radiolabeled, biological, and enzyme-linked immunosorbent assays for the pharmacokinetic study of PEGylated molecules.

Original languageEnglish
Pages (from-to)881-899
Number of pages19
JournalBioconjugate Chemistry
Volume23
Issue number5
DOIs
Publication statusPublished - May 16 2012
Externally publishedYes

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Pharmacokinetics
Drug products
Polyethylene glycols
Molecules
Assays
Pharmaceutical Preparations
Liposomes
Immunosorbents
Peptides
Ethylene Glycol
United States Food and Drug Administration
Enzymes
Nanoparticles
Proteins
Fluids
Enzyme-Linked Immunosorbent Assay
Chemical analysis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Analytical measurement of pegylated molecules. / Cheng, Tian Lu; Chuang, Kuo Hsiang; Chen, Bing Mae; Roffler, Steve R.

In: Bioconjugate Chemistry, Vol. 23, No. 5, 16.05.2012, p. 881-899.

Research output: Contribution to journalArticle

Cheng, TL, Chuang, KH, Chen, BM & Roffler, SR 2012, 'Analytical measurement of pegylated molecules', Bioconjugate Chemistry, vol. 23, no. 5, pp. 881-899. https://doi.org/10.1021/bc200478w
Cheng, Tian Lu ; Chuang, Kuo Hsiang ; Chen, Bing Mae ; Roffler, Steve R. / Analytical measurement of pegylated molecules. In: Bioconjugate Chemistry. 2012 ; Vol. 23, No. 5. pp. 881-899.
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