Analysis of the complete genome sequence of the Hz-1 virus suggests that it is related to members of the Baculoviridae

Chia Hsiung Cheng, Su Mei Liu, Teh Yuan Chow, Yu Yun Hsiao, Dan Ping Wang, Jiann Jang Huang, Hong Hwa Chen

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

We report the complete sequence of a large rod-shaped DNA virus, called the Hz-1 virus. This virus persistently infects the Heliothis zea cell lines. The Hz-1 virus has a double-stranded circular DNA genome of 228,089 bp encoding 154 open reading frames (ORFs) and also expresses a persistence-associated transcript 1, PAT1. The G+C content of the Hz-1 virus genome is 41.8%, with a gene density of one gene per 1.47 kb. Sequence analysis revealed that a 9.6-kb region at 43.6 to 47.8 map units harbors five cellular genes encoding proteins with homology to dUTP pyrophosphatase, matrix metalloproteinase, deoxynucleoside kinase, glycine hydroxymethyltransferase, and ribonucleotide reductase large subunit. Other cellular homologs were also detected dispersed in the viral genome. Several baculovirus homologs were detected in the Hz-1 virus genome. These include PxOrf-70, PxOrf-29, AcOrf-81, AcOrf-96, AcOrf-22, VLF-1, RNA polymerase LEF-8 (orf50), and two structural proteins, p74 and p91. The Hz-1 virus p74 homolog shows high structural conservation with a double transmembrane domain at its C terminus. Phylogenetic analysis of the p74 revealed that the Hz-1 virus is evolutionarily distant from the baculoviruses. Another distinctive feature of the Hz-1 virus genome is a gene that is involved in insect development. However, the remainder of the ORFs (81%) encoded proteins that bear no homology to any known proteins. In conclusion, the sequence differences between the Hz-1 virus and the baculoviruses outnumber the similarities and suggest that the Hz-1 virus may form a new family of viruses distantly related to the Baculoviridae.

Original languageEnglish
Pages (from-to)9024-9034
Number of pages11
JournalJournal of Virology
Volume76
Issue number18
DOIs
Publication statusPublished - Sep 2002
Externally publishedYes

Fingerprint

Baculoviridae
Genome
Viruses
genome
open reading frames
genes
Open Reading Frames
ribonucleotide reductase
glycine hydroxymethyltransferase
circular DNA
Proteins
pyrophosphatases
Heliothis zea nudivirus 1
DNA viruses
viruses
insect development
Glycine Hydroxymethyltransferase
Helicoverpa zea
proteins
new family

ASJC Scopus subject areas

  • Immunology

Cite this

Analysis of the complete genome sequence of the Hz-1 virus suggests that it is related to members of the Baculoviridae. / Cheng, Chia Hsiung; Liu, Su Mei; Chow, Teh Yuan; Hsiao, Yu Yun; Wang, Dan Ping; Huang, Jiann Jang; Chen, Hong Hwa.

In: Journal of Virology, Vol. 76, No. 18, 09.2002, p. 9024-9034.

Research output: Contribution to journalArticle

Cheng, Chia Hsiung ; Liu, Su Mei ; Chow, Teh Yuan ; Hsiao, Yu Yun ; Wang, Dan Ping ; Huang, Jiann Jang ; Chen, Hong Hwa. / Analysis of the complete genome sequence of the Hz-1 virus suggests that it is related to members of the Baculoviridae. In: Journal of Virology. 2002 ; Vol. 76, No. 18. pp. 9024-9034.
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