Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection

Triwit Rattanarojpong, Hao Ching Wang, Chu Fang Lo, Timothy W. Flegel

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In this proteomic analysis of gills from yellow head virus (YHV)-infected Penaeus vannamei, we identified 13 spots with up-regulated protein expression levels and five spots with down-regulated levels. LC-nanoESI-MS/MS indicated that the up-regulated proteins included enzymes in the glycolytic pathway, the tricarboxylic acid cycle and amino acid metabolism. The other up-regulated proteins were arginine kinase, imaginal disk growth factor (IDGF) and a Ras-like GTP binding protein. By contrast, expression levels were reduced for an SCP-calcium binding protein (SCP), actin-1, a valosin-containing protein, and Rab11. Time-course assays by real time RT-PCR revealed no significant increase in mRNA level of glycolytic enzymes and arginine kinase. However, a significant decrease in SCP mRNA was observed. The present results are consistent with previously published work and suggest that a decrease in SCP expression may play an important role in the shrimp response to viral infections in general.

Original languageEnglish
Pages (from-to)3809-3814
Number of pages6
JournalProteomics
Volume7
Issue number20
DOIs
Publication statusPublished - Oct 2007
Externally publishedYes

Keywords

  • Hemocyanin
  • LC-nanoESI-MS/MS
  • MASCOT program
  • RT-PCR
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection'. Together they form a unique fingerprint.

  • Cite this