Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection

Triwit Rattanarojpong, Hao Ching Wang, Chu Fang Lo, Timothy W. Flegel

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In this proteomic analysis of gills from yellow head virus (YHV)-infected Penaeus vannamei, we identified 13 spots with up-regulated protein expression levels and five spots with down-regulated levels. LC-nanoESI-MS/MS indicated that the up-regulated proteins included enzymes in the glycolytic pathway, the tricarboxylic acid cycle and amino acid metabolism. The other up-regulated proteins were arginine kinase, imaginal disk growth factor (IDGF) and a Ras-like GTP binding protein. By contrast, expression levels were reduced for an SCP-calcium binding protein (SCP), actin-1, a valosin-containing protein, and Rab11. Time-course assays by real time RT-PCR revealed no significant increase in mRNA level of glycolytic enzymes and arginine kinase. However, a significant decrease in SCP mRNA was observed. The present results are consistent with previously published work and suggest that a decrease in SCP expression may play an important role in the shrimp response to viral infections in general.

Original languageEnglish
Pages (from-to)3809-3814
Number of pages6
JournalProteomics
Volume7
Issue number20
DOIs
Publication statusPublished - Oct 2007
Externally publishedYes

Fingerprint

Roniviridae
Penaeidae
Virus Diseases
Arginine Kinase
Viruses
Imaginal Discs
Messenger RNA
Proteins
Calcium-Binding Proteins
Citric Acid Cycle
Enzymes
GTP-Binding Proteins
Metabolism
Proteomics
Actins
Real-Time Polymerase Chain Reaction
Assays
Intercellular Signaling Peptides and Proteins
Amino Acids

Keywords

  • Hemocyanin
  • LC-nanoESI-MS/MS
  • MASCOT program
  • RT-PCR
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection. / Rattanarojpong, Triwit; Wang, Hao Ching; Lo, Chu Fang; Flegel, Timothy W.

In: Proteomics, Vol. 7, No. 20, 10.2007, p. 3809-3814.

Research output: Contribution to journalArticle

Rattanarojpong, Triwit ; Wang, Hao Ching ; Lo, Chu Fang ; Flegel, Timothy W. / Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection. In: Proteomics. 2007 ; Vol. 7, No. 20. pp. 3809-3814.
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