Analysis of bcl-2 expression in normal, inflamed, dysplastic nasopharyngeal epithelia, and nasopharyngeal carcinoma: Association with p53 expression

Lai Fa Sheu, Ann Chen, Ching Liang Meng, Kuo Chieh Ho, Fu Gong Lin, Wei Hwa Lee

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

To further characterize bcl-2 expression in nasopharyngeal carcinoma (NPC), the authors analyzed bcl-2 expression immunohistochemically in biopsy specimens from 101 cases of NPC, of which 65 had the component of normal nasopharyngeal epithelium (NPE), 24 with dysplastic lesions adjacent to carcinoma, and 14 with both primary and metastatic lesions. An additional 25 nasopharyngeal biopsies of NPE from patients with chronic inflammation of nasopharynx were also included. The percentage of detectable bcl-2 expression shown in NPC (80%) and adjacent dysplastic lesions (71%) was significantly higher than in adjacent NPE (37%) and NPE from patients with chronic inflammation of the nasopharynx (30%) (P <.05). In both normal and inflamed NPE, the detectable bcl-2 expression was restricted to the basal cells; however, in dysplastic lesions, the bcl-2 staining distribution was increased with the dysplastic cell layers, and in entire layers of epithelium in severe dysplasia or carcinoma in situ. In addition, the staining intensity of bcl-2 in carcinomas and adjacent dysplastic lesions was generally stronger than that of adjacent NPE. These observations suggest that the expression of bcl- 2 in dysplasia and carcinoma is enhanced relative to that of adjacent NPE. Enhanced bcl-2 expression to prevent apoptosis seems to occur from die early stages and may play an important role in the carcinogenesis of NPC. Furthermore, up to 77% of NPC with die coexpression of bcl-2 and p53 was observed and suggested that the association of bcl-2 and p53 expression seems to occur from the early stages of the development of NPC. The overexpression of p53 protein in NPC suggests that the mutation of p53 gene or altered function of wild-type p53 protein may contribute to the pathogenesis. It is conceivable that the presence of both enhanced bcl-2 expression and altered p53 functions may play a crucial synergistic effect in die carcinogenesis of NPC.

Original languageEnglish
Pages (from-to)556-562
Number of pages7
JournalHuman Pathology
Volume28
Issue number5
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Epithelium
Nasopharynx
Carcinoma
Carcinogenesis
Staining and Labeling
Inflammation
Biopsy
Nasopharyngeal carcinoma
p53 Genes
Carcinoma in Situ
Proteins
Apoptosis
Mutation

Keywords

  • bcl-2
  • nasopharyngeal carcinoma
  • p53

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Analysis of bcl-2 expression in normal, inflamed, dysplastic nasopharyngeal epithelia, and nasopharyngeal carcinoma : Association with p53 expression. / Sheu, Lai Fa; Chen, Ann; Meng, Ching Liang; Ho, Kuo Chieh; Lin, Fu Gong; Lee, Wei Hwa.

In: Human Pathology, Vol. 28, No. 5, 1997, p. 556-562.

Research output: Contribution to journalArticle

Sheu, Lai Fa ; Chen, Ann ; Meng, Ching Liang ; Ho, Kuo Chieh ; Lin, Fu Gong ; Lee, Wei Hwa. / Analysis of bcl-2 expression in normal, inflamed, dysplastic nasopharyngeal epithelia, and nasopharyngeal carcinoma : Association with p53 expression. In: Human Pathology. 1997 ; Vol. 28, No. 5. pp. 556-562.
@article{1bc5ac7d92c44c3c9e222d862b6bf2dc,
title = "Analysis of bcl-2 expression in normal, inflamed, dysplastic nasopharyngeal epithelia, and nasopharyngeal carcinoma: Association with p53 expression",
abstract = "To further characterize bcl-2 expression in nasopharyngeal carcinoma (NPC), the authors analyzed bcl-2 expression immunohistochemically in biopsy specimens from 101 cases of NPC, of which 65 had the component of normal nasopharyngeal epithelium (NPE), 24 with dysplastic lesions adjacent to carcinoma, and 14 with both primary and metastatic lesions. An additional 25 nasopharyngeal biopsies of NPE from patients with chronic inflammation of nasopharynx were also included. The percentage of detectable bcl-2 expression shown in NPC (80{\%}) and adjacent dysplastic lesions (71{\%}) was significantly higher than in adjacent NPE (37{\%}) and NPE from patients with chronic inflammation of the nasopharynx (30{\%}) (P <.05). In both normal and inflamed NPE, the detectable bcl-2 expression was restricted to the basal cells; however, in dysplastic lesions, the bcl-2 staining distribution was increased with the dysplastic cell layers, and in entire layers of epithelium in severe dysplasia or carcinoma in situ. In addition, the staining intensity of bcl-2 in carcinomas and adjacent dysplastic lesions was generally stronger than that of adjacent NPE. These observations suggest that the expression of bcl- 2 in dysplasia and carcinoma is enhanced relative to that of adjacent NPE. Enhanced bcl-2 expression to prevent apoptosis seems to occur from die early stages and may play an important role in the carcinogenesis of NPC. Furthermore, up to 77{\%} of NPC with die coexpression of bcl-2 and p53 was observed and suggested that the association of bcl-2 and p53 expression seems to occur from the early stages of the development of NPC. The overexpression of p53 protein in NPC suggests that the mutation of p53 gene or altered function of wild-type p53 protein may contribute to the pathogenesis. It is conceivable that the presence of both enhanced bcl-2 expression and altered p53 functions may play a crucial synergistic effect in die carcinogenesis of NPC.",
keywords = "bcl-2, nasopharyngeal carcinoma, p53",
author = "Sheu, {Lai Fa} and Ann Chen and Meng, {Ching Liang} and Ho, {Kuo Chieh} and Lin, {Fu Gong} and Lee, {Wei Hwa}",
year = "1997",
doi = "10.1016/S0046-8177(97)90078-2",
language = "English",
volume = "28",
pages = "556--562",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders",
number = "5",

}

TY - JOUR

T1 - Analysis of bcl-2 expression in normal, inflamed, dysplastic nasopharyngeal epithelia, and nasopharyngeal carcinoma

T2 - Association with p53 expression

AU - Sheu, Lai Fa

AU - Chen, Ann

AU - Meng, Ching Liang

AU - Ho, Kuo Chieh

AU - Lin, Fu Gong

AU - Lee, Wei Hwa

PY - 1997

Y1 - 1997

N2 - To further characterize bcl-2 expression in nasopharyngeal carcinoma (NPC), the authors analyzed bcl-2 expression immunohistochemically in biopsy specimens from 101 cases of NPC, of which 65 had the component of normal nasopharyngeal epithelium (NPE), 24 with dysplastic lesions adjacent to carcinoma, and 14 with both primary and metastatic lesions. An additional 25 nasopharyngeal biopsies of NPE from patients with chronic inflammation of nasopharynx were also included. The percentage of detectable bcl-2 expression shown in NPC (80%) and adjacent dysplastic lesions (71%) was significantly higher than in adjacent NPE (37%) and NPE from patients with chronic inflammation of the nasopharynx (30%) (P <.05). In both normal and inflamed NPE, the detectable bcl-2 expression was restricted to the basal cells; however, in dysplastic lesions, the bcl-2 staining distribution was increased with the dysplastic cell layers, and in entire layers of epithelium in severe dysplasia or carcinoma in situ. In addition, the staining intensity of bcl-2 in carcinomas and adjacent dysplastic lesions was generally stronger than that of adjacent NPE. These observations suggest that the expression of bcl- 2 in dysplasia and carcinoma is enhanced relative to that of adjacent NPE. Enhanced bcl-2 expression to prevent apoptosis seems to occur from die early stages and may play an important role in the carcinogenesis of NPC. Furthermore, up to 77% of NPC with die coexpression of bcl-2 and p53 was observed and suggested that the association of bcl-2 and p53 expression seems to occur from the early stages of the development of NPC. The overexpression of p53 protein in NPC suggests that the mutation of p53 gene or altered function of wild-type p53 protein may contribute to the pathogenesis. It is conceivable that the presence of both enhanced bcl-2 expression and altered p53 functions may play a crucial synergistic effect in die carcinogenesis of NPC.

AB - To further characterize bcl-2 expression in nasopharyngeal carcinoma (NPC), the authors analyzed bcl-2 expression immunohistochemically in biopsy specimens from 101 cases of NPC, of which 65 had the component of normal nasopharyngeal epithelium (NPE), 24 with dysplastic lesions adjacent to carcinoma, and 14 with both primary and metastatic lesions. An additional 25 nasopharyngeal biopsies of NPE from patients with chronic inflammation of nasopharynx were also included. The percentage of detectable bcl-2 expression shown in NPC (80%) and adjacent dysplastic lesions (71%) was significantly higher than in adjacent NPE (37%) and NPE from patients with chronic inflammation of the nasopharynx (30%) (P <.05). In both normal and inflamed NPE, the detectable bcl-2 expression was restricted to the basal cells; however, in dysplastic lesions, the bcl-2 staining distribution was increased with the dysplastic cell layers, and in entire layers of epithelium in severe dysplasia or carcinoma in situ. In addition, the staining intensity of bcl-2 in carcinomas and adjacent dysplastic lesions was generally stronger than that of adjacent NPE. These observations suggest that the expression of bcl- 2 in dysplasia and carcinoma is enhanced relative to that of adjacent NPE. Enhanced bcl-2 expression to prevent apoptosis seems to occur from die early stages and may play an important role in the carcinogenesis of NPC. Furthermore, up to 77% of NPC with die coexpression of bcl-2 and p53 was observed and suggested that the association of bcl-2 and p53 expression seems to occur from the early stages of the development of NPC. The overexpression of p53 protein in NPC suggests that the mutation of p53 gene or altered function of wild-type p53 protein may contribute to the pathogenesis. It is conceivable that the presence of both enhanced bcl-2 expression and altered p53 functions may play a crucial synergistic effect in die carcinogenesis of NPC.

KW - bcl-2

KW - nasopharyngeal carcinoma

KW - p53

UR - http://www.scopus.com/inward/record.url?scp=0030919604&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030919604&partnerID=8YFLogxK

U2 - 10.1016/S0046-8177(97)90078-2

DO - 10.1016/S0046-8177(97)90078-2

M3 - Article

C2 - 9158704

AN - SCOPUS:0030919604

VL - 28

SP - 556

EP - 562

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 5

ER -