Analgesic effects and the mechanisms of anti-inflammation of ergostatrien-3β-ol from antrodia camphorata submerged whole broth in mice

Guan Jhong Huang, Shyh Shyun Huang, Shiang Shiou Lin, Yi Yuan Shao, Chin Chu Chen, Wen Chi Hou, Yueh Hsiung Kuo

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Ergostatrien-3β-ol (ST1), an active and major ingredient from Antrodia camphorata (AC) submerged whole broth was evaluated for the analgesic and anti-inflammatory effects. Treatment of male imprinting control region (ICR) mice with ST1 (1, 5, and 10 mg/kg) significantly inhibited the numbers of acetic-acid-induced writhing response in 10 min. Also, our result showed that ST1 (10 mg/kg) significantly inhibited the formalin-induced pain in the late phase (p <0.001). In the anti-inflammatory test, ST1 (10 mg/kg) decreased the paw edema at 4 and 5 h after λ-carrageenin (Carr) administration and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. We also demonstrated that ST1 significantly attenuated the malondialdehyde (MDA) level in the edema paw at 5 h after Carr injection. ST1 (1, 5, and 10 mg/kg) decreased the nitric oxide (NO) levels on both the edema paw and serum level at 5 h after Carr injection. Also, ST1 (5 and 10 mg/kg) diminished the serum tumor necrosis factor (TNF-α) at 5 h after Carr injection. Western blotting revealed that ST1 (10 mg/kg) decreased Carr-induced inducible nitric oxide synthase (iNOS), and cycloxyclase (COX-2) expressions at 5 h in the edema paw. An intraperitoneal (ip) injection treatment with ST1 also diminished neutrophil infiltration into sites of inflammation, as did indomethacin (Indo). The anti-inflammatory mechanisms of ST1 might be related to the decrease in the level of MDA, iNOS, and COX-2 in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver through the suppression of TNF-α and NO.

Original languageEnglish
Pages (from-to)7445-7452
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number12
DOIs
Publication statusPublished - Jun 23 2010

Fingerprint

Antrodia
Taiwanofungus camphoratus
analgesic effect
Carrageenan
carrageenan
edema
Analgesics
Edema
inflammation
Inflammation
mice
tumor necrosis factors
Nitric Oxide Synthase Type II
prostaglandin synthase
Glutathione Peroxidase
injection
Malondialdehyde
glutathione peroxidase
Liver
Catalase

Keywords

  • Analgesic
  • Anti-inflammation
  • Chinese herb
  • Ergostatrien-3β-ol
  • MDA
  • NO
  • TNF-α

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)
  • Medicine(all)

Cite this

Analgesic effects and the mechanisms of anti-inflammation of ergostatrien-3β-ol from antrodia camphorata submerged whole broth in mice. / Huang, Guan Jhong; Huang, Shyh Shyun; Lin, Shiang Shiou; Shao, Yi Yuan; Chen, Chin Chu; Hou, Wen Chi; Kuo, Yueh Hsiung.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 12, 23.06.2010, p. 7445-7452.

Research output: Contribution to journalArticle

Huang, Guan Jhong ; Huang, Shyh Shyun ; Lin, Shiang Shiou ; Shao, Yi Yuan ; Chen, Chin Chu ; Hou, Wen Chi ; Kuo, Yueh Hsiung. / Analgesic effects and the mechanisms of anti-inflammation of ergostatrien-3β-ol from antrodia camphorata submerged whole broth in mice. In: Journal of Agricultural and Food Chemistry. 2010 ; Vol. 58, No. 12. pp. 7445-7452.
@article{dbce7ff6bb0d4dd0a7bffcd0695b7763,
title = "Analgesic effects and the mechanisms of anti-inflammation of ergostatrien-3β-ol from antrodia camphorata submerged whole broth in mice",
abstract = "Ergostatrien-3β-ol (ST1), an active and major ingredient from Antrodia camphorata (AC) submerged whole broth was evaluated for the analgesic and anti-inflammatory effects. Treatment of male imprinting control region (ICR) mice with ST1 (1, 5, and 10 mg/kg) significantly inhibited the numbers of acetic-acid-induced writhing response in 10 min. Also, our result showed that ST1 (10 mg/kg) significantly inhibited the formalin-induced pain in the late phase (p <0.001). In the anti-inflammatory test, ST1 (10 mg/kg) decreased the paw edema at 4 and 5 h after λ-carrageenin (Carr) administration and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. We also demonstrated that ST1 significantly attenuated the malondialdehyde (MDA) level in the edema paw at 5 h after Carr injection. ST1 (1, 5, and 10 mg/kg) decreased the nitric oxide (NO) levels on both the edema paw and serum level at 5 h after Carr injection. Also, ST1 (5 and 10 mg/kg) diminished the serum tumor necrosis factor (TNF-α) at 5 h after Carr injection. Western blotting revealed that ST1 (10 mg/kg) decreased Carr-induced inducible nitric oxide synthase (iNOS), and cycloxyclase (COX-2) expressions at 5 h in the edema paw. An intraperitoneal (ip) injection treatment with ST1 also diminished neutrophil infiltration into sites of inflammation, as did indomethacin (Indo). The anti-inflammatory mechanisms of ST1 might be related to the decrease in the level of MDA, iNOS, and COX-2 in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver through the suppression of TNF-α and NO.",
keywords = "Analgesic, Anti-inflammation, Chinese herb, Ergostatrien-3β-ol, MDA, NO, TNF-α",
author = "Huang, {Guan Jhong} and Huang, {Shyh Shyun} and Lin, {Shiang Shiou} and Shao, {Yi Yuan} and Chen, {Chin Chu} and Hou, {Wen Chi} and Kuo, {Yueh Hsiung}",
year = "2010",
month = "6",
day = "23",
doi = "10.1021/jf1013764",
language = "English",
volume = "58",
pages = "7445--7452",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - Analgesic effects and the mechanisms of anti-inflammation of ergostatrien-3β-ol from antrodia camphorata submerged whole broth in mice

AU - Huang, Guan Jhong

AU - Huang, Shyh Shyun

AU - Lin, Shiang Shiou

AU - Shao, Yi Yuan

AU - Chen, Chin Chu

AU - Hou, Wen Chi

AU - Kuo, Yueh Hsiung

PY - 2010/6/23

Y1 - 2010/6/23

N2 - Ergostatrien-3β-ol (ST1), an active and major ingredient from Antrodia camphorata (AC) submerged whole broth was evaluated for the analgesic and anti-inflammatory effects. Treatment of male imprinting control region (ICR) mice with ST1 (1, 5, and 10 mg/kg) significantly inhibited the numbers of acetic-acid-induced writhing response in 10 min. Also, our result showed that ST1 (10 mg/kg) significantly inhibited the formalin-induced pain in the late phase (p <0.001). In the anti-inflammatory test, ST1 (10 mg/kg) decreased the paw edema at 4 and 5 h after λ-carrageenin (Carr) administration and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. We also demonstrated that ST1 significantly attenuated the malondialdehyde (MDA) level in the edema paw at 5 h after Carr injection. ST1 (1, 5, and 10 mg/kg) decreased the nitric oxide (NO) levels on both the edema paw and serum level at 5 h after Carr injection. Also, ST1 (5 and 10 mg/kg) diminished the serum tumor necrosis factor (TNF-α) at 5 h after Carr injection. Western blotting revealed that ST1 (10 mg/kg) decreased Carr-induced inducible nitric oxide synthase (iNOS), and cycloxyclase (COX-2) expressions at 5 h in the edema paw. An intraperitoneal (ip) injection treatment with ST1 also diminished neutrophil infiltration into sites of inflammation, as did indomethacin (Indo). The anti-inflammatory mechanisms of ST1 might be related to the decrease in the level of MDA, iNOS, and COX-2 in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver through the suppression of TNF-α and NO.

AB - Ergostatrien-3β-ol (ST1), an active and major ingredient from Antrodia camphorata (AC) submerged whole broth was evaluated for the analgesic and anti-inflammatory effects. Treatment of male imprinting control region (ICR) mice with ST1 (1, 5, and 10 mg/kg) significantly inhibited the numbers of acetic-acid-induced writhing response in 10 min. Also, our result showed that ST1 (10 mg/kg) significantly inhibited the formalin-induced pain in the late phase (p <0.001). In the anti-inflammatory test, ST1 (10 mg/kg) decreased the paw edema at 4 and 5 h after λ-carrageenin (Carr) administration and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. We also demonstrated that ST1 significantly attenuated the malondialdehyde (MDA) level in the edema paw at 5 h after Carr injection. ST1 (1, 5, and 10 mg/kg) decreased the nitric oxide (NO) levels on both the edema paw and serum level at 5 h after Carr injection. Also, ST1 (5 and 10 mg/kg) diminished the serum tumor necrosis factor (TNF-α) at 5 h after Carr injection. Western blotting revealed that ST1 (10 mg/kg) decreased Carr-induced inducible nitric oxide synthase (iNOS), and cycloxyclase (COX-2) expressions at 5 h in the edema paw. An intraperitoneal (ip) injection treatment with ST1 also diminished neutrophil infiltration into sites of inflammation, as did indomethacin (Indo). The anti-inflammatory mechanisms of ST1 might be related to the decrease in the level of MDA, iNOS, and COX-2 in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver through the suppression of TNF-α and NO.

KW - Analgesic

KW - Anti-inflammation

KW - Chinese herb

KW - Ergostatrien-3β-ol

KW - MDA

KW - NO

KW - TNF-α

UR - http://www.scopus.com/inward/record.url?scp=77953639975&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953639975&partnerID=8YFLogxK

U2 - 10.1021/jf1013764

DO - 10.1021/jf1013764

M3 - Article

C2 - 20507140

AN - SCOPUS:77953639975

VL - 58

SP - 7445

EP - 7452

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 12

ER -