Analgesic effects and the mechanisms of anti-inflammation of ergostatrien-3β-ol from antrodia camphorata submerged whole broth in mice

Guan Jhong Huang, Shyh Shyun Huang, Shiang Shiou Lin, Yi Yuan Shao, Chin Chu Chen, Wen Chi Hou, Yueh Hsiung Kuo

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Ergostatrien-3β-ol (ST1), an active and major ingredient from Antrodia camphorata (AC) submerged whole broth was evaluated for the analgesic and anti-inflammatory effects. Treatment of male imprinting control region (ICR) mice with ST1 (1, 5, and 10 mg/kg) significantly inhibited the numbers of acetic-acid-induced writhing response in 10 min. Also, our result showed that ST1 (10 mg/kg) significantly inhibited the formalin-induced pain in the late phase (p <0.001). In the anti-inflammatory test, ST1 (10 mg/kg) decreased the paw edema at 4 and 5 h after λ-carrageenin (Carr) administration and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. We also demonstrated that ST1 significantly attenuated the malondialdehyde (MDA) level in the edema paw at 5 h after Carr injection. ST1 (1, 5, and 10 mg/kg) decreased the nitric oxide (NO) levels on both the edema paw and serum level at 5 h after Carr injection. Also, ST1 (5 and 10 mg/kg) diminished the serum tumor necrosis factor (TNF-α) at 5 h after Carr injection. Western blotting revealed that ST1 (10 mg/kg) decreased Carr-induced inducible nitric oxide synthase (iNOS), and cycloxyclase (COX-2) expressions at 5 h in the edema paw. An intraperitoneal (ip) injection treatment with ST1 also diminished neutrophil infiltration into sites of inflammation, as did indomethacin (Indo). The anti-inflammatory mechanisms of ST1 might be related to the decrease in the level of MDA, iNOS, and COX-2 in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver through the suppression of TNF-α and NO.

Original languageEnglish
Pages (from-to)7445-7452
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number12
DOIs
Publication statusPublished - Jun 23 2010

Keywords

  • Analgesic
  • Anti-inflammation
  • Chinese herb
  • Ergostatrien-3β-ol
  • MDA
  • NO
  • TNF-α

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)
  • Medicine(all)

Fingerprint Dive into the research topics of 'Analgesic effects and the mechanisms of anti-inflammation of ergostatrien-3β-ol from antrodia camphorata submerged whole broth in mice'. Together they form a unique fingerprint.

  • Cite this