Analgesic And Anti-Inflammatory Activities Of An Ethanol Extract Of Dunaliella Salina Teod. (Chlorophyceae)

Pei Yu Chou, Guan Jhong Huang, Hsu Chen Cheng, Chieh Hsi Wu, Yi Chung Chien, Jwo Sheng Chen, Ming Hsing Huang, Kai Jeng Hsu, Ming Jyh Sheu

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8 Citations (Scopus)


This study investigated the analgesic and anti-inflammatory effects of an ethanol extract of Dunaliella salina Teod. (Chlorophyceae) (EDS) in Imprinting Control Region mice. Standard all-trans-β-carotene and the amount of all-trans-β-carotene in an EDS were analyzed by high-performance liquid chromatography (HPLC). In HPLC analysis, the fingerprint chromatogram of EDS was established. Both all-trans-β-carotene and EDS showed similar peaks at the retention time of 24 min. This implied that EDS contained the active ingredient all-trans-β-carotene. Treatment of animals with EDS significantly inhibited the numbers of acetic acid-induced writhing responses at doses of 0.5 g/kg (P <0.01), 1.0 g/kg (P <0.001) and 2.0 g/kg (P <0.001). This inhibitory effect of EDS (1.0 and 2.0 g/kg) on acetic acid-induced writhings was similar to that of the positive control indomethacin (10 mg/kg) (P <0.001). EDS did not significantly inhibit the formalin-induced pain in the early phase; however, at doses of 0.1 g/kg (P <0.01), 0.5, 1.0 and 2.0 g/kg, EDS significantly inhibited the formalin-induced pain in the late phase (P <0.001). Finally, EDS at doses of 1.0 and 2.0 g/kg also inhibited the development of paw edema induced by λ-carrageenan (carrageenan). EDS (1.0 and 2.0 mg/kg) decreased the level of nitiric oxide (NO) in edematous paw tissue and in serum level, and diminished the level of serum tumor necrosis factor-α (TNF-α) at the fifth hour after carrageenan injection. Based on these findings, EDS probably exerts anti-inflammatory effects by suppressing TNF-α and NO. These results suggest that EDS might be a potential pharmacological analgesic and anti-inflammatory agent.

Original languageEnglish
Pages (from-to)1288-1302
Number of pages15
JournalJournal of Food Biochemistry
Issue number6
Publication statusPublished - Dec 2010
Externally publishedYes

ASJC Scopus subject areas

  • Food Science
  • Cell Biology
  • Pharmacology
  • Biophysics


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