Analgesic and anti-inflammatory activities of a water extract of Trachelospermum jasminoides (Apocynaceae)

Ming Jyh Sheu, Pei Yu Chou, Hsu Chen Cheng, Chieh Hsi Wu, Guan Jhong Huang, Bor Sen Wang, Jwo Sheng Chen, Yi Chung Chien, Ming Hsing Huang

Research output: Contribution to journalArticle

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Abstract

Aims of the study: This study investigated the analgesic and anti-inflammatory effects of a water extract of Trachelospermum jasminoides (WET) in ICR mice. Materials and methods: In HPLC analysis, the fingerprint chromatogram of WET was established. Acetic acid-induced writhing response and formalin-induced pain were examined the analgesics effects of WET. WET on λ-Carrageenan(carr)-induced paw edema was performed. We investigate the anti-inflammatory mechanism of WET via studies of the activities of glutathione peroxidase (GPx), glutathione reductase (GRx) in the liver and the levels of malondialdehyde (MDA) and nitrite oxide (NO) in the edema paw. Serum NO and TNF-α were also measured. Results: The fingerprint chromatogram of WET was established through HPLC analysis, and implies that WET contains the active ingredient gallic acid, chlorgenic acid, caffeic acid, taxifolin, isoquercitrin and quercetin. WET significantly inhibited the numbers of acetic acid-induced writhing responses and the formalin-induced pain in the late phase. In the anti-inflammatory test, WET inhibited the development of paw edema induced by carr. WET decreased the paw edema at the third, fourth and fifth hour after carr administration, and increased the activities of SOD, GPx and GRx in the liver tissue and decreased the MDA level in the edema paw at the third hour after carr injection. WET decreased the level of NO in edematous paw tissue and in serum level, and diminished the level of serum TNF-α at the fifth hour after carr injection. Conclusions: These results demonstrated that WET is an effective anti-inflammatory agent in carr-induced inflammation. WET probably exerts anti-inflammatory effects by suppressing TNF-α and NO. The anti-inflammatory mechanism of WET might be related to the decrease in the level of MDA in the edema paw via increasing the activities of SOD, GPx and GRx in the liver.

Original languageEnglish
Pages (from-to)332-338
Number of pages7
JournalJournal of Ethnopharmacology
Volume126
Issue number2
DOIs
Publication statusPublished - Nov 12 2009
Externally publishedYes

Fingerprint

Apocynaceae
Non-Steroidal Anti-Inflammatory Agents
Water
Carrageenan
Edema
Nitrites
Anti-Inflammatory Agents
Oxides
Glutathione Peroxidase
Malondialdehyde
Dermatoglyphics
Acetic Acid
Formaldehyde
Liver
Serum
High Pressure Liquid Chromatography
Pain
Inbred ICR Mouse
Gallic Acid
Injections

Keywords

  • Analgesic
  • Anti-inflammation
  • MDA
  • NO
  • TNF-α
  • Trachelospermum jasminoides

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Analgesic and anti-inflammatory activities of a water extract of Trachelospermum jasminoides (Apocynaceae). / Sheu, Ming Jyh; Chou, Pei Yu; Cheng, Hsu Chen; Wu, Chieh Hsi; Huang, Guan Jhong; Wang, Bor Sen; Chen, Jwo Sheng; Chien, Yi Chung; Huang, Ming Hsing.

In: Journal of Ethnopharmacology, Vol. 126, No. 2, 12.11.2009, p. 332-338.

Research output: Contribution to journalArticle

Sheu, MJ, Chou, PY, Cheng, HC, Wu, CH, Huang, GJ, Wang, BS, Chen, JS, Chien, YC & Huang, MH 2009, 'Analgesic and anti-inflammatory activities of a water extract of Trachelospermum jasminoides (Apocynaceae)', Journal of Ethnopharmacology, vol. 126, no. 2, pp. 332-338. https://doi.org/10.1016/j.jep.2009.08.019
Sheu, Ming Jyh ; Chou, Pei Yu ; Cheng, Hsu Chen ; Wu, Chieh Hsi ; Huang, Guan Jhong ; Wang, Bor Sen ; Chen, Jwo Sheng ; Chien, Yi Chung ; Huang, Ming Hsing. / Analgesic and anti-inflammatory activities of a water extract of Trachelospermum jasminoides (Apocynaceae). In: Journal of Ethnopharmacology. 2009 ; Vol. 126, No. 2. pp. 332-338.
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