An ultra-weak chemiluminescence study on oxidative stress in rabbits following acute thermal injury

Jui Sheng Sun, Yang Hwei Tsuang, I. Jen Chen, Wan Ching Huang, Yi Shiong Hang, Fung Jou Lu

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

It is not easy to detect oxygen free radicals directly because of their very short half-life. In the present study, a sensitive ultraweak chemiluminescence detector was used to detect the generation of oxygen free radicals following thermal injury. Twelve New Zealand white rabbits were used in this study. After anesthesia, the bilateral hind-limbs were exposed to 100°C water for 30 s. Six control animals were exposed to 22°C water to act as a control. The chemiluminescence of whole blood and visceral organs were measured with both luminol-amplified t-butyl hydroperoxide-initiated and lucigenin-initiated methods. The results showed that chemiluminescence of blood was affected significantly by acute thermal injury. The chemiluminescence of blood increased significantly at 1 h following acute thermal injury, reached a peak at 2 h, then decreased but still remained above the control level at 4 h following thermal injury. The results for TBHP-initiated chemiluminescence from visceral organs following acute thermal injury were much higher than that of the control rabbits. The effects of lucigenin-initiated tissue chemiluminescence following acute thermal injury were not statistically significant. It is suggested that the decreased vascular antioxidant activity following local thermal injury is partially contributed by the superoxide pathway while, the remote pathophysiologic events are mediated by the defective scavenging defenses.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalBurns
Volume24
Issue number3
DOIs
Publication statusPublished - May 1998
Externally publishedYes

Fingerprint

Luminescence
Oxidative Stress
Hot Temperature
Rabbits
Wounds and Injuries
Free Radicals
Reactive Oxygen Species
tert-Butylhydroperoxide
Luminol
Water
Superoxides
Blood Vessels
Half-Life
Extremities
Anesthesia
Antioxidants

Keywords

  • Burn model
  • Free radicals
  • Oxidative stress

ASJC Scopus subject areas

  • Emergency Medicine
  • Surgery

Cite this

An ultra-weak chemiluminescence study on oxidative stress in rabbits following acute thermal injury. / Sun, Jui Sheng; Tsuang, Yang Hwei; Chen, I. Jen; Huang, Wan Ching; Hang, Yi Shiong; Lu, Fung Jou.

In: Burns, Vol. 24, No. 3, 05.1998, p. 225-231.

Research output: Contribution to journalArticle

Sun, Jui Sheng ; Tsuang, Yang Hwei ; Chen, I. Jen ; Huang, Wan Ching ; Hang, Yi Shiong ; Lu, Fung Jou. / An ultra-weak chemiluminescence study on oxidative stress in rabbits following acute thermal injury. In: Burns. 1998 ; Vol. 24, No. 3. pp. 225-231.
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