An integrated system of RBCs sorting and Brownian motion bio-sensing technique

Yu Jui Fan, Ching Jiun Lee, Jen Hao Hsu, Yi Hsing Liu, Szu I. Yeh, Horn Jiunn Sheen, Shiming Lin, Jing Fa Tsai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using oxygen-plasma direct bonding method, the assembly of an electrode chip and the microchannels will form a set of RBCs and nanobeads separating chip. The original concentration of antigen can be rapidly estimated by nanobeads' Brownian motion measurement in varies of the viscous solutions. During the antibody-antigen reacting, the mean value of the beads diameter increased so that the Brownian velocity decreased with increasing time. The Brownian motion of nanobeads was observed and measured by micro-particle tracking velocimetry (μ-PTV).

Original languageEnglish
Title of host publication2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Pages487-491
Number of pages5
DOIs
Publication statusPublished - 2010
Externally publishedYes
Event5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 - Xiamen, China
Duration: Jan 20 2010Jan 23 2010

Conference

Conference5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
CountryChina
CityXiamen
Period1/20/101/23/10

Fingerprint

Brownian movement
Antigens
Sorting
Antibodies
Plasmas
Microchannels
Electrophoresis
Velocity measurement
MEMS
Masks
Blood
Proteins
Fabrication
Electrodes
Oxygen
Experiments

Keywords

  • Biosensing
  • Brownian motion
  • Dielectrophoresis (DEP)
  • Micro-particle tracking velocimetry (μ-PTV)

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Fan, Y. J., Lee, C. J., Hsu, J. H., Liu, Y. H., Yeh, S. I., Sheen, H. J., ... Tsai, J. F. (2010). An integrated system of RBCs sorting and Brownian motion bio-sensing technique. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 (pp. 487-491). [5592441] https://doi.org/10.1109/NEMS.2010.5592441

An integrated system of RBCs sorting and Brownian motion bio-sensing technique. / Fan, Yu Jui; Lee, Ching Jiun; Hsu, Jen Hao; Liu, Yi Hsing; Yeh, Szu I.; Sheen, Horn Jiunn; Lin, Shiming; Tsai, Jing Fa.

2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. p. 487-491 5592441.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fan, YJ, Lee, CJ, Hsu, JH, Liu, YH, Yeh, SI, Sheen, HJ, Lin, S & Tsai, JF 2010, An integrated system of RBCs sorting and Brownian motion bio-sensing technique. in 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010., 5592441, pp. 487-491, 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, Xiamen, China, 1/20/10. https://doi.org/10.1109/NEMS.2010.5592441
Fan YJ, Lee CJ, Hsu JH, Liu YH, Yeh SI, Sheen HJ et al. An integrated system of RBCs sorting and Brownian motion bio-sensing technique. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. p. 487-491. 5592441 https://doi.org/10.1109/NEMS.2010.5592441
Fan, Yu Jui ; Lee, Ching Jiun ; Hsu, Jen Hao ; Liu, Yi Hsing ; Yeh, Szu I. ; Sheen, Horn Jiunn ; Lin, Shiming ; Tsai, Jing Fa. / An integrated system of RBCs sorting and Brownian motion bio-sensing technique. 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. pp. 487-491
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