An integrated system of RBCs sorting and Brownian motion bio-sensing technique

Yu Jui Fan, Ching Jiun Lee, Jen Hao Hsu, Yi Hsing Liu, Szu I. Yeh, Horn Jiunn Sheen, Shiming Lin, Jing Fa Tsai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A clinical sorting chip integrated with new protein sensing technique has been developed in this work. Antibody-coated nanobeads reacted with antigen was separated from blood plasma based on dielectrophoresis (DEP) forces. MEMS fabrication process was used to produce the bio-chip and only two photo masks were required in the experiment. By using oxygen-plasma direct bonding method, the assembly of an electrode chip and the microchannels will form a set of RBCs and nanobeads separating chip. The original concentration of antigen can be rapidly estimated by nanobeads' Brownian motion measurement in varies of the viscous solutions. During the antibody-antigen reacting, the mean value of the beads diameter increased so that the Brownian velocity decreased with increasing time. The Brownian motion of nanobeads was observed and measured by micro-particle tracking velocimetry (μ-PTV).

Original languageEnglish
Title of host publication2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Pages487-491
Number of pages5
DOIs
Publication statusPublished - 2010
Externally publishedYes
Event5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 - Xiamen, China
Duration: Jan 20 2010Jan 23 2010

Conference

Conference5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
CountryChina
CityXiamen
Period1/20/101/23/10

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Keywords

  • Biosensing
  • Brownian motion
  • Dielectrophoresis (DEP)
  • Micro-particle tracking velocimetry (μ-PTV)

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Fan, Y. J., Lee, C. J., Hsu, J. H., Liu, Y. H., Yeh, S. I., Sheen, H. J., Lin, S., & Tsai, J. F. (2010). An integrated system of RBCs sorting and Brownian motion bio-sensing technique. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 (pp. 487-491). [5592441] https://doi.org/10.1109/NEMS.2010.5592441