日本血吸蟲26 KDa麩胺基硫轉移酶應用於人體日本血吸蟲症之免疫診斷

Translated title of the contribution: An Evaluation of Recombinant 26 KDa Glutathione S-transferase of Schistosoma Japonicum as an Antigen for Serodiagnosis in Human Schistosomiasis Japonica

Po-Ching Cheng, WH Chou, J.-J. Huang, Sho Masanoyi, KM Lee

Research output: Contribution to journalArticle

Abstract

Schistosoma japonicum is a zoonotic trematode, which is endemic in Indonesia, Philippines and mainland China. The most serious country is mainland China. Schistosomiasis japonica causes pathological lesions in several organs. Morbidity is primarily due to deposition and entrapment of parasite eggs within host tissues which induce a delayed hypersensitivity granulomatous responses, leading to gross enlargement of the liver, spleen and intestine, fibrosis, and eventually hepatic destruction, cirrhosis and liver failure in chronic infection. This study develops 26KDa Glutathione S-transferase recombinant protein by using RT-PCR technology, and uses this antigen as immunodiagnostic reagent for human schistosomiasis japonica. The results showed that the O.D. values of serum samples from acute and chronic patients were statistically significant higher than the O.D. values of serum samples from healthy peoples in endemic and non-endemic areas. No immune cross reactions were found in acute and chronic patients compared with the patients infected with Paragonimus westermani, hookworms and Ascaris lumbricoides. These results indicated that this recombinant 26kDa GST might be a good immunodiagnostic antigen for human schistosomiasis japonica.
Original languageTraditional Chinese
Pages (from-to)147-170
Journal中臺學報
Volume19
Issue number1
Publication statusPublished - 2007

Fingerprint

Schistosomiasis japonica
Schistosoma japonicum
Serologic Tests
Glutathione Transferase
Liver Failure
Antigens
China
Paragonimus westermani
Ascaris lumbricoides
Ancylostomatoidea
Philippines
Indonesia
Cross Reactions
Zoonoses
Delayed Hypersensitivity
Serum
Recombinant Proteins
Liver Cirrhosis
Eggs
Intestines

Keywords

  • Schistosomiasis Japonica
  • Immunodiagnostic reagent
  • Glutathione S-transferase
  • RT-PCR
  • Specificity and sensitivity

Cite this

Cheng, P-C., Chou, WH., Huang, J-J., Masanoyi, S., & Lee, KM. (2007). 日本血吸蟲26 KDa麩胺基硫轉移酶應用於人體日本血吸蟲症之免疫診斷. 中臺學報, 19(1), 147-170.

日本血吸蟲26 KDa麩胺基硫轉移酶應用於人體日本血吸蟲症之免疫診斷. / Cheng, Po-Ching; Chou, WH; Huang, J.-J.; Masanoyi, Sho; Lee, KM.

In: 中臺學報, Vol. 19, No. 1, 2007, p. 147-170.

Research output: Contribution to journalArticle

Cheng, P-C, Chou, WH, Huang, J-J, Masanoyi, S & Lee, KM 2007, '日本血吸蟲26 KDa麩胺基硫轉移酶應用於人體日本血吸蟲症之免疫診斷', 中臺學報, vol. 19, no. 1, pp. 147-170.
Cheng, Po-Ching ; Chou, WH ; Huang, J.-J. ; Masanoyi, Sho ; Lee, KM. / 日本血吸蟲26 KDa麩胺基硫轉移酶應用於人體日本血吸蟲症之免疫診斷. In: 中臺學報. 2007 ; Vol. 19, No. 1. pp. 147-170.
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abstract = "日本血吸蟲病為人畜共通寄生蟲疾病,流行於印尼、菲律賓及中國大陸等亞洲國家,其中以中國大陸最為嚴重。日本血吸蟲病會導致人類及動物的各種組織性疾病,亦即在蟲卵周圍之宿主組織如肝、脾、肺、腸等器官會形成肉芽腫性的高度過敏性反應,造成肝脾腫大,最後患者往往因肝硬化、腹水形成、門靜脈血流阻塞等病症死亡。本研究應用反轉錄聚合酶連鎖反應技術開發大陸株日本血吸蟲26KDa Glutathione S-transferase(GST, 麩胺基硫轉移酶)重組蛋白,並應用於人體日本血吸蟲病免疫檢測試驗。研究結果顯示,日本血吸蟲急性病患與慢性病患組血清樣本的O.D.值遠高於疫區正常人與非疫區正常人組的O.D.值,統計上具顯著差異。在血清交叉反應實驗上,同樣地,血吸蟲急性與慢性病患組與肺吸蟲病患組、鉤蟲病患組以及蛔蟲病患組無免疫交叉反應發生。此研究結果顯示,26KDa GST重組蛋白可用來作為日本血吸蟲感染症的免疫檢測診斷試劑,且此重組蛋白可辨識感染其他寄生蟲病患的血清,在臨床診斷上無交叉反應現象。",
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KW - 免疫診斷試劑

KW - 麩胺基硫轉移酶

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KW - 專一性及靈敏度

KW - Schistosomiasis Japonica

KW - Immunodiagnostic reagent

KW - Glutathione S-transferase

KW - RT-PCR

KW - Specificity and sensitivity

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