Abstract

Recently, a novel substrate known as an electrospun polylactic acid (PLLA) microtube array membrane (MTAM) was successfully developed as a cell coculture platform. Structurally, this substrate is made up of one-to-one connected, ultrathin, submicron scale fibers that are arranged in an arrayed formation. Its unique structure confers several key advantages which are beneficial in a cell coculture system. In this study, the interaction between rat fetal neural stem cells (NSC) and astrocytes was examined by comparing the outcome of a typical Transwell-based coculture system and that of an electrospun PLLA MTAM-based coculture system. Compared to tissue culture polystyrene (TCP) and Transwell coculture inserts, a superior cell viability of NSC was observed when cultured in lumens of electrospun PLLA MTAM (with supportive immunostaining images). Reverse transcription polymerase chain reaction revealed a strong interaction between astrocytes and NSC through a higher expression of doublecortin and a lower expression of nestin. These data demonstrate that MTAM is clearly a better coculture platform than the traditional Transwell system.

Original languageEnglish
Pages (from-to)179-190
Number of pages12
JournalCells Tissues Organs
Volume204
Issue number3-4
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Nerve Regeneration
Coculture Techniques
Membranes
Neural Stem Cells
Astrocytes
Fetal Stem Cells
Nestin
Polystyrenes
Reverse Transcription
Cell Survival
Polymerase Chain Reaction
poly(lactic acid)

Keywords

  • Astrocytes
  • Coculture
  • Electrospinning
  • Microtube array membrane
  • Polylactic acid
  • Rat fetal neural stem cell

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration. / Tseng, V. Chia Hsuan; Chew, Chee Ho; Huang, Wan Ting; Wang, Yang Kao; Chen, Ko Shao; Chou, Szu Yi; Chen, Chien Chung.

In: Cells Tissues Organs, Vol. 204, No. 3-4, 01.10.2017, p. 179-190.

Research output: Contribution to journalArticle

Tseng, V. Chia Hsuan ; Chew, Chee Ho ; Huang, Wan Ting ; Wang, Yang Kao ; Chen, Ko Shao ; Chou, Szu Yi ; Chen, Chien Chung. / An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration. In: Cells Tissues Organs. 2017 ; Vol. 204, No. 3-4. pp. 179-190.
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