An association between human papillomavirus 16/18 deoxyribonucleic acid in peripheral blood with p16 protein expression in neoplastic cervical lesions

Horng J. Tsai, Ya W. Peng, Long Y. Lin, Ming Chih Chou, Huei Lee, Hui Ling Chiou

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Human papillomavirus (HPV) infection plays a crucial role in cervical carcinogenesis. Apart from the detection of p16 protein in cervical tissues, the feasibility of the presence of HPV DNA in peripheral blood being an auxiliary marker of cervical lesions was examined. Methods: Peripheral blood samples and cervical tissues, from 36 cervical tissues from high-grade squamous intraepithelial lesions (HSIL) and 31 early invasive cervical cancers (EICC), were analyzed for HPV 16/18 DNA and HPV 16/18 E7 mRNA expression, as well as the in situ expressions of p16 and pRb to investigate the in-between associations. Results: The prevalence of HPV 16/18 DNA in patients with EICC was relatively higher than those of HSIL, in both of cervical tissues and peripheral blood. The presence of HPV 16/18 DNA in peripheral blood was positively correlated with that in cervical tissue, as well as with p16 overexpression in cervical tissues together with a significant correlation between E7 mRNA and pRb and p16 protein expressions. Discussion: A positive correlation between the presence of HPV 16 or 18 DNA in peripheral blood and p16 overexpression in tissues of patients with cervical lesions was confirmed. Together with p16 immunostaining in cervical tissues, the detection of high-risk HPV 16 or 18 DNA in peripheral blood may act as an auxiliary biomarker for HPV-associated neoplastic cervical lesions.

Original languageEnglish
Pages (from-to)537-543
Number of pages7
JournalCancer Detection and Prevention
Volume29
Issue number6
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Human papillomavirus 18
Human papillomavirus 16
DNA
Proteins
Uterine Cervical Neoplasms
Messenger RNA
Papillomavirus Infections
Carcinogenesis
Biomarkers

Keywords

  • HPV 16/18 DNA
  • Neoplastic cervical lesions
  • p16
  • Peripheral blood

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

An association between human papillomavirus 16/18 deoxyribonucleic acid in peripheral blood with p16 protein expression in neoplastic cervical lesions. / Tsai, Horng J.; Peng, Ya W.; Lin, Long Y.; Chou, Ming Chih; Lee, Huei; Chiou, Hui Ling.

In: Cancer Detection and Prevention, Vol. 29, No. 6, 2005, p. 537-543.

Research output: Contribution to journalArticle

Tsai, Horng J. ; Peng, Ya W. ; Lin, Long Y. ; Chou, Ming Chih ; Lee, Huei ; Chiou, Hui Ling. / An association between human papillomavirus 16/18 deoxyribonucleic acid in peripheral blood with p16 protein expression in neoplastic cervical lesions. In: Cancer Detection and Prevention. 2005 ; Vol. 29, No. 6. pp. 537-543.
@article{39e5531faa5c4519b44fb82340d666ec,
title = "An association between human papillomavirus 16/18 deoxyribonucleic acid in peripheral blood with p16 protein expression in neoplastic cervical lesions",
abstract = "Background: Human papillomavirus (HPV) infection plays a crucial role in cervical carcinogenesis. Apart from the detection of p16 protein in cervical tissues, the feasibility of the presence of HPV DNA in peripheral blood being an auxiliary marker of cervical lesions was examined. Methods: Peripheral blood samples and cervical tissues, from 36 cervical tissues from high-grade squamous intraepithelial lesions (HSIL) and 31 early invasive cervical cancers (EICC), were analyzed for HPV 16/18 DNA and HPV 16/18 E7 mRNA expression, as well as the in situ expressions of p16 and pRb to investigate the in-between associations. Results: The prevalence of HPV 16/18 DNA in patients with EICC was relatively higher than those of HSIL, in both of cervical tissues and peripheral blood. The presence of HPV 16/18 DNA in peripheral blood was positively correlated with that in cervical tissue, as well as with p16 overexpression in cervical tissues together with a significant correlation between E7 mRNA and pRb and p16 protein expressions. Discussion: A positive correlation between the presence of HPV 16 or 18 DNA in peripheral blood and p16 overexpression in tissues of patients with cervical lesions was confirmed. Together with p16 immunostaining in cervical tissues, the detection of high-risk HPV 16 or 18 DNA in peripheral blood may act as an auxiliary biomarker for HPV-associated neoplastic cervical lesions.",
keywords = "HPV 16/18 DNA, Neoplastic cervical lesions, p16, Peripheral blood",
author = "Tsai, {Horng J.} and Peng, {Ya W.} and Lin, {Long Y.} and Chou, {Ming Chih} and Huei Lee and Chiou, {Hui Ling}",
year = "2005",
doi = "10.1016/j.cdp.2005.09.008",
language = "English",
volume = "29",
pages = "537--543",
journal = "Cancer Epidemiology",
issn = "1877-7821",
publisher = "Elsevier BV",
number = "6",

}

TY - JOUR

T1 - An association between human papillomavirus 16/18 deoxyribonucleic acid in peripheral blood with p16 protein expression in neoplastic cervical lesions

AU - Tsai, Horng J.

AU - Peng, Ya W.

AU - Lin, Long Y.

AU - Chou, Ming Chih

AU - Lee, Huei

AU - Chiou, Hui Ling

PY - 2005

Y1 - 2005

N2 - Background: Human papillomavirus (HPV) infection plays a crucial role in cervical carcinogenesis. Apart from the detection of p16 protein in cervical tissues, the feasibility of the presence of HPV DNA in peripheral blood being an auxiliary marker of cervical lesions was examined. Methods: Peripheral blood samples and cervical tissues, from 36 cervical tissues from high-grade squamous intraepithelial lesions (HSIL) and 31 early invasive cervical cancers (EICC), were analyzed for HPV 16/18 DNA and HPV 16/18 E7 mRNA expression, as well as the in situ expressions of p16 and pRb to investigate the in-between associations. Results: The prevalence of HPV 16/18 DNA in patients with EICC was relatively higher than those of HSIL, in both of cervical tissues and peripheral blood. The presence of HPV 16/18 DNA in peripheral blood was positively correlated with that in cervical tissue, as well as with p16 overexpression in cervical tissues together with a significant correlation between E7 mRNA and pRb and p16 protein expressions. Discussion: A positive correlation between the presence of HPV 16 or 18 DNA in peripheral blood and p16 overexpression in tissues of patients with cervical lesions was confirmed. Together with p16 immunostaining in cervical tissues, the detection of high-risk HPV 16 or 18 DNA in peripheral blood may act as an auxiliary biomarker for HPV-associated neoplastic cervical lesions.

AB - Background: Human papillomavirus (HPV) infection plays a crucial role in cervical carcinogenesis. Apart from the detection of p16 protein in cervical tissues, the feasibility of the presence of HPV DNA in peripheral blood being an auxiliary marker of cervical lesions was examined. Methods: Peripheral blood samples and cervical tissues, from 36 cervical tissues from high-grade squamous intraepithelial lesions (HSIL) and 31 early invasive cervical cancers (EICC), were analyzed for HPV 16/18 DNA and HPV 16/18 E7 mRNA expression, as well as the in situ expressions of p16 and pRb to investigate the in-between associations. Results: The prevalence of HPV 16/18 DNA in patients with EICC was relatively higher than those of HSIL, in both of cervical tissues and peripheral blood. The presence of HPV 16/18 DNA in peripheral blood was positively correlated with that in cervical tissue, as well as with p16 overexpression in cervical tissues together with a significant correlation between E7 mRNA and pRb and p16 protein expressions. Discussion: A positive correlation between the presence of HPV 16 or 18 DNA in peripheral blood and p16 overexpression in tissues of patients with cervical lesions was confirmed. Together with p16 immunostaining in cervical tissues, the detection of high-risk HPV 16 or 18 DNA in peripheral blood may act as an auxiliary biomarker for HPV-associated neoplastic cervical lesions.

KW - HPV 16/18 DNA

KW - Neoplastic cervical lesions

KW - p16

KW - Peripheral blood

UR - http://www.scopus.com/inward/record.url?scp=28744452493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28744452493&partnerID=8YFLogxK

U2 - 10.1016/j.cdp.2005.09.008

DO - 10.1016/j.cdp.2005.09.008

M3 - Article

C2 - 16289504

AN - SCOPUS:28744452493

VL - 29

SP - 537

EP - 543

JO - Cancer Epidemiology

JF - Cancer Epidemiology

SN - 1877-7821

IS - 6

ER -