An association between BPDE-like DNA adduct levels and P53 gene mutation in pterygium

Te J. Lai, Yi Y. Tsai, Ya W. Cheng, Chun C. Chiang, Huei Lee, Ming Chih Chou, Julia Hueimei Chang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: A previous report of ours noted that not only p53 protein overexpression, but also p53 gene mutation. were indeed detected in pterygium. BaP 7,8-diol 9,10-epoxide (BPDE), an ultimate metabolite of BaP, attacks deoxyguanosine to form a BPDE-N2-dG adduct resulting in p53 mutations. The relationship between BPDE-like DNA adduct levels and abnormal p53 has not been clear in pterygium. Therefore, BPDE-like DNA adduct, p53 protein expression and p53 gene mutation were examined in this study to provide more molecular evidence to understand the cause of p53 gene mutation in pterygium. Methods: In this study, immunohistochemical staining, using a monoclonal antibody (DO7) against p53 and a polyclonal antibody against BPDE-like DNA adducts, was performed on 73 pterygial specimens. DNA samples for p53 mutation analysis were extracted from epithelial cells and then subjected to DNA sequencing for the determination of mutations in exons 4, 5, 6, 7, and 8 of the p53 gene. Results: BPDE-like DNA adducts were detected in 36.1% (26/73) pterygium samples. No correlation between adduct levels and p53 protein expression was found in these samples. Additionally, the p53 gene mutation and p53 mutation pattern also did not correlate with BPDE-like DNA adduct levels. Conclusions: Our data provides evidence that BPDE-like DNA adducts are indeed detected in pterygium samples, and they are only minor contributors to the abnormal p53 gene.

Original languageEnglish
Pages (from-to)1687-1691
Number of pages5
JournalMolecular Vision
Volume12
Publication statusPublished - Dec 24 2006
Externally publishedYes

Fingerprint

7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
Pterygium
DNA Adducts
p53 Genes
Mutation
Proteins
Deoxyguanosine
DNA Sequence Analysis
Exons
Epithelial Cells
Monoclonal Antibodies
Staining and Labeling

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Lai, T. J., Tsai, Y. Y., Cheng, Y. W., Chiang, C. C., Lee, H., Chou, M. C., & Chang, J. H. (2006). An association between BPDE-like DNA adduct levels and P53 gene mutation in pterygium. Molecular Vision, 12, 1687-1691.

An association between BPDE-like DNA adduct levels and P53 gene mutation in pterygium. / Lai, Te J.; Tsai, Yi Y.; Cheng, Ya W.; Chiang, Chun C.; Lee, Huei; Chou, Ming Chih; Chang, Julia Hueimei.

In: Molecular Vision, Vol. 12, 24.12.2006, p. 1687-1691.

Research output: Contribution to journalArticle

Lai, TJ, Tsai, YY, Cheng, YW, Chiang, CC, Lee, H, Chou, MC & Chang, JH 2006, 'An association between BPDE-like DNA adduct levels and P53 gene mutation in pterygium', Molecular Vision, vol. 12, pp. 1687-1691.
Lai TJ, Tsai YY, Cheng YW, Chiang CC, Lee H, Chou MC et al. An association between BPDE-like DNA adduct levels and P53 gene mutation in pterygium. Molecular Vision. 2006 Dec 24;12:1687-1691.
Lai, Te J. ; Tsai, Yi Y. ; Cheng, Ya W. ; Chiang, Chun C. ; Lee, Huei ; Chou, Ming Chih ; Chang, Julia Hueimei. / An association between BPDE-like DNA adduct levels and P53 gene mutation in pterygium. In: Molecular Vision. 2006 ; Vol. 12. pp. 1687-1691.
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N2 - Purpose: A previous report of ours noted that not only p53 protein overexpression, but also p53 gene mutation. were indeed detected in pterygium. BaP 7,8-diol 9,10-epoxide (BPDE), an ultimate metabolite of BaP, attacks deoxyguanosine to form a BPDE-N2-dG adduct resulting in p53 mutations. The relationship between BPDE-like DNA adduct levels and abnormal p53 has not been clear in pterygium. Therefore, BPDE-like DNA adduct, p53 protein expression and p53 gene mutation were examined in this study to provide more molecular evidence to understand the cause of p53 gene mutation in pterygium. Methods: In this study, immunohistochemical staining, using a monoclonal antibody (DO7) against p53 and a polyclonal antibody against BPDE-like DNA adducts, was performed on 73 pterygial specimens. DNA samples for p53 mutation analysis were extracted from epithelial cells and then subjected to DNA sequencing for the determination of mutations in exons 4, 5, 6, 7, and 8 of the p53 gene. Results: BPDE-like DNA adducts were detected in 36.1% (26/73) pterygium samples. No correlation between adduct levels and p53 protein expression was found in these samples. Additionally, the p53 gene mutation and p53 mutation pattern also did not correlate with BPDE-like DNA adduct levels. Conclusions: Our data provides evidence that BPDE-like DNA adducts are indeed detected in pterygium samples, and they are only minor contributors to the abnormal p53 gene.

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