An association between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 polymorphisma in pterygium

Jai Nien Tung, Heng Hsiung Wu, Chun C. Chiang, Yi Y. Tsai, Ming Chih Chou, Huei Lee, Ya W. Cheng

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: Benzo[a]pyrene 7,8-diol 9,10-epoxide (BPDE), an ultimate metabolite of benzo[a]pyrene, attacks deoxyguanosine to form a BPDE-N2-dG adduct resulting in p53 mutations. Both cytochrome P4501A1 (CYP1A1) and glutathione S-transferase M1 (GSTM1) have been demonstrated to be involved in the metabolism of polycyclic aromatic hydrocarbons. The relationship between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 gene polymorphisms in pterygium is not clear. Therefore, BPDE-like DNA adducts and CYP1A1 and GSTM1 polymorphisms were detected in this study to provide more molecular evidence to understand the cause of BPDE-like DNA adduct formation in pterygium. Methods: In this study, immunohistochemical staining using a polyclonal antibody on BPDE-like DNA adducts was performed on 103 pterygial specimens. For the analysis of CYP1A1 and GSTM1 polymorphisms, DNA samples were extracted from epithelial cells and then subjected to restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) for the determination of mutation and genotype of CYP1A1 and GSTM1. Results: BPDE-like DNA adducts were detected in 33.0% (34/103) of the pterygium samples. The differences in DNA adduct levels were associated with the genetic polymorphisms of CYP1A1 but not GSTM1. Additionally, the risk of BPDElike DNA adduct formation for patients with CYP1A1 m1/m2 (C/T) and m2/m2 (T/T) was 9.675 fold higher than that of patients with CYP1A1 m1/m1 (C/C) types (p=0.001, 95% Confidence Interval 2.451-38.185). Conclusions: Our data provide evidence that the BPDE-like DNA adduct formation in pterygium samples was associated with CYP1A1 polymorphisms.

Original languageEnglish
Pages (from-to)623-629
Number of pages7
JournalMolecular Vision
Volume16
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Pterygium
DNA Adducts
Cytochromes
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA
glutathione S-transferase M1
Mutation
Deoxyguanosine
Benzo(a)pyrene
Polycyclic Aromatic Hydrocarbons
Genetic Polymorphisms
Restriction Fragment Length Polymorphisms
Epithelial Cells
Genotype
Confidence Intervals
Staining and Labeling
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Tung, J. N., Wu, H. H., Chiang, C. C., Tsai, Y. Y., Chou, M. C., Lee, H., & Cheng, Y. W. (2010). An association between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 polymorphisma in pterygium. Molecular Vision, 16, 623-629.

An association between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 polymorphisma in pterygium. / Tung, Jai Nien; Wu, Heng Hsiung; Chiang, Chun C.; Tsai, Yi Y.; Chou, Ming Chih; Lee, Huei; Cheng, Ya W.

In: Molecular Vision, Vol. 16, 2010, p. 623-629.

Research output: Contribution to journalArticle

Tung, JN, Wu, HH, Chiang, CC, Tsai, YY, Chou, MC, Lee, H & Cheng, YW 2010, 'An association between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 polymorphisma in pterygium', Molecular Vision, vol. 16, pp. 623-629.
Tung JN, Wu HH, Chiang CC, Tsai YY, Chou MC, Lee H et al. An association between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 polymorphisma in pterygium. Molecular Vision. 2010;16:623-629.
Tung, Jai Nien ; Wu, Heng Hsiung ; Chiang, Chun C. ; Tsai, Yi Y. ; Chou, Ming Chih ; Lee, Huei ; Cheng, Ya W. / An association between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 polymorphisma in pterygium. In: Molecular Vision. 2010 ; Vol. 16. pp. 623-629.
@article{aae1bba0f00848eeb65f0d5ca3a572bf,
title = "An association between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 polymorphisma in pterygium",
abstract = "Purpose: Benzo[a]pyrene 7,8-diol 9,10-epoxide (BPDE), an ultimate metabolite of benzo[a]pyrene, attacks deoxyguanosine to form a BPDE-N2-dG adduct resulting in p53 mutations. Both cytochrome P4501A1 (CYP1A1) and glutathione S-transferase M1 (GSTM1) have been demonstrated to be involved in the metabolism of polycyclic aromatic hydrocarbons. The relationship between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 gene polymorphisms in pterygium is not clear. Therefore, BPDE-like DNA adducts and CYP1A1 and GSTM1 polymorphisms were detected in this study to provide more molecular evidence to understand the cause of BPDE-like DNA adduct formation in pterygium. Methods: In this study, immunohistochemical staining using a polyclonal antibody on BPDE-like DNA adducts was performed on 103 pterygial specimens. For the analysis of CYP1A1 and GSTM1 polymorphisms, DNA samples were extracted from epithelial cells and then subjected to restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) for the determination of mutation and genotype of CYP1A1 and GSTM1. Results: BPDE-like DNA adducts were detected in 33.0{\%} (34/103) of the pterygium samples. The differences in DNA adduct levels were associated with the genetic polymorphisms of CYP1A1 but not GSTM1. Additionally, the risk of BPDElike DNA adduct formation for patients with CYP1A1 m1/m2 (C/T) and m2/m2 (T/T) was 9.675 fold higher than that of patients with CYP1A1 m1/m1 (C/C) types (p=0.001, 95{\%} Confidence Interval 2.451-38.185). Conclusions: Our data provide evidence that the BPDE-like DNA adduct formation in pterygium samples was associated with CYP1A1 polymorphisms.",
author = "Tung, {Jai Nien} and Wu, {Heng Hsiung} and Chiang, {Chun C.} and Tsai, {Yi Y.} and Chou, {Ming Chih} and Huei Lee and Cheng, {Ya W.}",
year = "2010",
language = "English",
volume = "16",
pages = "623--629",
journal = "Molecular Vision",
issn = "1090-0535",

}

TY - JOUR

T1 - An association between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 polymorphisma in pterygium

AU - Tung, Jai Nien

AU - Wu, Heng Hsiung

AU - Chiang, Chun C.

AU - Tsai, Yi Y.

AU - Chou, Ming Chih

AU - Lee, Huei

AU - Cheng, Ya W.

PY - 2010

Y1 - 2010

N2 - Purpose: Benzo[a]pyrene 7,8-diol 9,10-epoxide (BPDE), an ultimate metabolite of benzo[a]pyrene, attacks deoxyguanosine to form a BPDE-N2-dG adduct resulting in p53 mutations. Both cytochrome P4501A1 (CYP1A1) and glutathione S-transferase M1 (GSTM1) have been demonstrated to be involved in the metabolism of polycyclic aromatic hydrocarbons. The relationship between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 gene polymorphisms in pterygium is not clear. Therefore, BPDE-like DNA adducts and CYP1A1 and GSTM1 polymorphisms were detected in this study to provide more molecular evidence to understand the cause of BPDE-like DNA adduct formation in pterygium. Methods: In this study, immunohistochemical staining using a polyclonal antibody on BPDE-like DNA adducts was performed on 103 pterygial specimens. For the analysis of CYP1A1 and GSTM1 polymorphisms, DNA samples were extracted from epithelial cells and then subjected to restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) for the determination of mutation and genotype of CYP1A1 and GSTM1. Results: BPDE-like DNA adducts were detected in 33.0% (34/103) of the pterygium samples. The differences in DNA adduct levels were associated with the genetic polymorphisms of CYP1A1 but not GSTM1. Additionally, the risk of BPDElike DNA adduct formation for patients with CYP1A1 m1/m2 (C/T) and m2/m2 (T/T) was 9.675 fold higher than that of patients with CYP1A1 m1/m1 (C/C) types (p=0.001, 95% Confidence Interval 2.451-38.185). Conclusions: Our data provide evidence that the BPDE-like DNA adduct formation in pterygium samples was associated with CYP1A1 polymorphisms.

AB - Purpose: Benzo[a]pyrene 7,8-diol 9,10-epoxide (BPDE), an ultimate metabolite of benzo[a]pyrene, attacks deoxyguanosine to form a BPDE-N2-dG adduct resulting in p53 mutations. Both cytochrome P4501A1 (CYP1A1) and glutathione S-transferase M1 (GSTM1) have been demonstrated to be involved in the metabolism of polycyclic aromatic hydrocarbons. The relationship between BPDE-like DNA adduct levels and CYP1A1 and GSTM1 gene polymorphisms in pterygium is not clear. Therefore, BPDE-like DNA adducts and CYP1A1 and GSTM1 polymorphisms were detected in this study to provide more molecular evidence to understand the cause of BPDE-like DNA adduct formation in pterygium. Methods: In this study, immunohistochemical staining using a polyclonal antibody on BPDE-like DNA adducts was performed on 103 pterygial specimens. For the analysis of CYP1A1 and GSTM1 polymorphisms, DNA samples were extracted from epithelial cells and then subjected to restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) for the determination of mutation and genotype of CYP1A1 and GSTM1. Results: BPDE-like DNA adducts were detected in 33.0% (34/103) of the pterygium samples. The differences in DNA adduct levels were associated with the genetic polymorphisms of CYP1A1 but not GSTM1. Additionally, the risk of BPDElike DNA adduct formation for patients with CYP1A1 m1/m2 (C/T) and m2/m2 (T/T) was 9.675 fold higher than that of patients with CYP1A1 m1/m1 (C/C) types (p=0.001, 95% Confidence Interval 2.451-38.185). Conclusions: Our data provide evidence that the BPDE-like DNA adduct formation in pterygium samples was associated with CYP1A1 polymorphisms.

UR - http://www.scopus.com/inward/record.url?scp=77955619317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955619317&partnerID=8YFLogxK

M3 - Article

VL - 16

SP - 623

EP - 629

JO - Molecular Vision

JF - Molecular Vision

SN - 1090-0535

ER -