An androgen-regulated miRNA suppresses Bak1 expression and induces androgen-independent growth of prostate cancer cells

Xu Bao Shi, Lingru Xue, Joy Yang, Ai Hong Ma, Jianjun Zhao, Ma Xu, Clifford G. Tepper, Christopher P. Evans, Hsing Jien Kung, Ralph W DeVere White

Research output: Contribution to journalArticle

366 Citations (Scopus)

Abstract

Although prostate cancer (CaP) is the most frequently diagnosed malignant tumor and the second leading cause of cancer deaths in American men, the mechanisms explaining the development and progression of CaP remain largely unknown. Recent studies have shown that some aberrantly expressed microRNAs (miRNAs) are involved in tumorigenesis. Although aberrant expression of certain miRNAs has been discovered in CaP, their function in this disease has not yet been defined. In this study, we found differential expression of miR-125b in androgen-dependent and independent CaP cells, as well as in benign and malignant prostate tissues. Furthermore, androgen signaling was able to up-regulate the expression of miR-125b. In addition, transfection of synthetic miR-125b stimulated androgen-independent growth of CaP cells and down-regulated the expression of Bak1. Our results suggest that miR-125b acts as an oncogene, contributing to the pathogenesis of CaP.

Original languageEnglish
Pages (from-to)19983-19988
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number50
DOIs
Publication statusPublished - Dec 11 2007
Externally publishedYes

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Keywords

  • ISH
  • LNCaP
  • microRNA
  • miR-125b

ASJC Scopus subject areas

  • Genetics
  • General

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