An analysis of cytokine status in the serum and effusions of patients with tuberculous and lung cancer

Yuh Min Chen, Wen Kuang Yang, Jacqueline Whang-Peng, Chun Ming Tsai, Reury Perng Perng

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The present study was designed to ascertain whether or not the pleural effusion and serum cytokine levels (granulocyte-macrophage colony-stimulating factor [GM-CSF], interleukin-10 [IL-10], and interferon-γ [IFNγ]) in lung cancer patients differ from tuberculous (TB) pleural effusion, in which a strong cellular immune reaction is found; and, whether cytokine levels are a prognostic factor in lung cancer patients with malignant effusion. A total of 202 lung cancer patients with malignant pleural effusion and 26 patients with TB pleural effusion were studied consecutively between 1995 and 1998. Serum and effusion cytokine levels were analyzed with ELISA assays. The results showed that pleural effusion GM-CSF and IL-10 levels were significantly higher than serum levels in both cancer and TB patients. Pleural effusion IFNγ levels were significantly higher than serum levels in TB patients. IFNγ levels in both pleural effusion and serum were significantly higher in TB patients than in those with cancer. No significant difference was found, between TB and cancer patients, in the serum or pleural effusion levels of either IL-10 or GM-CSF. The ratio of pleural effusion IFNγ to serum IFNγ, effusion IFNγ to effusion IL-10, and effusion IL-10 to serum IL-10, were all significantly higher in TB than in cancer patients, suggesting a higher cellular activity and T-helper 1 (Th1) reaction in TB pleural effusion than in malignant effusions, which were predominantly Th2 type. Survival analysis showed no significant difference in lung cancer patients with different levels of these cytokines. It was concluded that lung cancer patients with malignant pleural effusion had poorer immune profiles than those with TB pleurisy, both locally and systemically; and the cytokine profiles were not prognostic factors for lung cancer patients with malignant pleural effusion.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalLung Cancer
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

Fingerprint

Lung Neoplasms
Pleural Effusion
Cytokines
Serum
Interleukin-10
Interferons
Malignant Pleural Effusion
Granulocyte-Macrophage Colony-Stimulating Factor
Neoplasms
Pleural Tuberculosis
Survival Analysis
Enzyme-Linked Immunosorbent Assay

Keywords

  • Colony-stimulating factor
  • Granulocyte-macrophage
  • Interferon-γ
  • Interleukin-10
  • Lung cancer
  • Tuberculosis

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine
  • Cancer Research

Cite this

An analysis of cytokine status in the serum and effusions of patients with tuberculous and lung cancer. / Chen, Yuh Min; Yang, Wen Kuang; Whang-Peng, Jacqueline; Tsai, Chun Ming; Perng, Reury Perng.

In: Lung Cancer, Vol. 31, No. 1, 01.01.2001, p. 25-30.

Research output: Contribution to journalArticle

Chen, Yuh Min ; Yang, Wen Kuang ; Whang-Peng, Jacqueline ; Tsai, Chun Ming ; Perng, Reury Perng. / An analysis of cytokine status in the serum and effusions of patients with tuberculous and lung cancer. In: Lung Cancer. 2001 ; Vol. 31, No. 1. pp. 25-30.
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abstract = "The present study was designed to ascertain whether or not the pleural effusion and serum cytokine levels (granulocyte-macrophage colony-stimulating factor [GM-CSF], interleukin-10 [IL-10], and interferon-γ [IFNγ]) in lung cancer patients differ from tuberculous (TB) pleural effusion, in which a strong cellular immune reaction is found; and, whether cytokine levels are a prognostic factor in lung cancer patients with malignant effusion. A total of 202 lung cancer patients with malignant pleural effusion and 26 patients with TB pleural effusion were studied consecutively between 1995 and 1998. Serum and effusion cytokine levels were analyzed with ELISA assays. The results showed that pleural effusion GM-CSF and IL-10 levels were significantly higher than serum levels in both cancer and TB patients. Pleural effusion IFNγ levels were significantly higher than serum levels in TB patients. IFNγ levels in both pleural effusion and serum were significantly higher in TB patients than in those with cancer. No significant difference was found, between TB and cancer patients, in the serum or pleural effusion levels of either IL-10 or GM-CSF. The ratio of pleural effusion IFNγ to serum IFNγ, effusion IFNγ to effusion IL-10, and effusion IL-10 to serum IL-10, were all significantly higher in TB than in cancer patients, suggesting a higher cellular activity and T-helper 1 (Th1) reaction in TB pleural effusion than in malignant effusions, which were predominantly Th2 type. Survival analysis showed no significant difference in lung cancer patients with different levels of these cytokines. It was concluded that lung cancer patients with malignant pleural effusion had poorer immune profiles than those with TB pleurisy, both locally and systemically; and the cytokine profiles were not prognostic factors for lung cancer patients with malignant pleural effusion.",
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