An amphipathic protein from sweet pepper can dissociate harpin(Pss) multimeric forms and intensify the harpin(Pss)-mediated hypersensitive response

Cheng-Hsien Chen, Hao Jan Lin, Teng Yung Feng

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17 Citations (Scopus)

Abstract

Harpin(Pss), a proteinaceous elicitor from Pseudomonas syringae pv. syringae (Pss), can induce a hypersensitive response (HR) in certain plants (e.g. tobacco and tomato). In this report, we show that an amphipathic hypersensitive response assisting protein (HRAP), purified from sweet pepper intensified the harpin(Pss)-mediated HR in sweet pepper, a plant normally not strongly affected by harpin(Pss). The molecular weight of HRAP was approximately 29 kDa, indicated by SDS-PAGE analysis, and the isoelectric point 8.6. The activity of HRAP was highly dependent on the ratio of harpin(Pss) to HRAP. In FPLC analysis we found that harpin(Pss) was present as monomer, dimer, trimer, tetramer, and octamer forms in neutral pH buffer systems. Among these native forms, only the monomer and dimer had HR eliciting activities. In the presence of HRAP, multimeric harpin(Pss) dissociated into dimeric and monomeric forms. HRAP had no proteolytic activity against harpin(Pss). The possible significance of the interaction between HRAP and the harpin(Pss) elicitor is discussed.

Original languageEnglish
Pages (from-to)139-149
Number of pages11
JournalPhysiological and Molecular Plant Pathology
Volume52
Issue number3
DOIs
Publication statusPublished - Mar 1998
Externally publishedYes

Fingerprint

Pseudomonas syringae pv. syringae
Capsicum
hypersensitive response
sweet peppers
Proteins
proteins
Pseudomonas syringae
Isoelectric Point
Lycopersicon esculentum
Tobacco
Polyacrylamide Gel Electrophoresis
Buffers
Molecular Weight
isoelectric point
tobacco
buffers
tomatoes
molecular weight

ASJC Scopus subject areas

  • Plant Science

Cite this

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abstract = "Harpin(Pss), a proteinaceous elicitor from Pseudomonas syringae pv. syringae (Pss), can induce a hypersensitive response (HR) in certain plants (e.g. tobacco and tomato). In this report, we show that an amphipathic hypersensitive response assisting protein (HRAP), purified from sweet pepper intensified the harpin(Pss)-mediated HR in sweet pepper, a plant normally not strongly affected by harpin(Pss). The molecular weight of HRAP was approximately 29 kDa, indicated by SDS-PAGE analysis, and the isoelectric point 8.6. The activity of HRAP was highly dependent on the ratio of harpin(Pss) to HRAP. In FPLC analysis we found that harpin(Pss) was present as monomer, dimer, trimer, tetramer, and octamer forms in neutral pH buffer systems. Among these native forms, only the monomer and dimer had HR eliciting activities. In the presence of HRAP, multimeric harpin(Pss) dissociated into dimeric and monomeric forms. HRAP had no proteolytic activity against harpin(Pss). The possible significance of the interaction between HRAP and the harpin(Pss) elicitor is discussed.",
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N2 - Harpin(Pss), a proteinaceous elicitor from Pseudomonas syringae pv. syringae (Pss), can induce a hypersensitive response (HR) in certain plants (e.g. tobacco and tomato). In this report, we show that an amphipathic hypersensitive response assisting protein (HRAP), purified from sweet pepper intensified the harpin(Pss)-mediated HR in sweet pepper, a plant normally not strongly affected by harpin(Pss). The molecular weight of HRAP was approximately 29 kDa, indicated by SDS-PAGE analysis, and the isoelectric point 8.6. The activity of HRAP was highly dependent on the ratio of harpin(Pss) to HRAP. In FPLC analysis we found that harpin(Pss) was present as monomer, dimer, trimer, tetramer, and octamer forms in neutral pH buffer systems. Among these native forms, only the monomer and dimer had HR eliciting activities. In the presence of HRAP, multimeric harpin(Pss) dissociated into dimeric and monomeric forms. HRAP had no proteolytic activity against harpin(Pss). The possible significance of the interaction between HRAP and the harpin(Pss) elicitor is discussed.

AB - Harpin(Pss), a proteinaceous elicitor from Pseudomonas syringae pv. syringae (Pss), can induce a hypersensitive response (HR) in certain plants (e.g. tobacco and tomato). In this report, we show that an amphipathic hypersensitive response assisting protein (HRAP), purified from sweet pepper intensified the harpin(Pss)-mediated HR in sweet pepper, a plant normally not strongly affected by harpin(Pss). The molecular weight of HRAP was approximately 29 kDa, indicated by SDS-PAGE analysis, and the isoelectric point 8.6. The activity of HRAP was highly dependent on the ratio of harpin(Pss) to HRAP. In FPLC analysis we found that harpin(Pss) was present as monomer, dimer, trimer, tetramer, and octamer forms in neutral pH buffer systems. Among these native forms, only the monomer and dimer had HR eliciting activities. In the presence of HRAP, multimeric harpin(Pss) dissociated into dimeric and monomeric forms. HRAP had no proteolytic activity against harpin(Pss). The possible significance of the interaction between HRAP and the harpin(Pss) elicitor is discussed.

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