Amphetamine activates connexin43 gene expression in cultured neonatal rat cardiomyocytes through JNK and AP-1 pathway

Kou Gi Shyu, Bao Wei Wang, Yu Hui Yang, Shiow Chwen Tsai, Shankung Lin, Chun Chung Lee

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objective: Amphetamine has been known to induce cardiac dysrhythmia and sudden death. However, the molecular mechanism for the induction of dysrhythmia is not known. Connexin43 (Cx43) plays an important role for arrhythmogenesis. This study was undertaken to test the hypothesis that amphetamine could induce Cx43 expression in cardiac myocytes. Methods: Neonatal Wistar rat cardiac myocytes were cultured under the stimulation of amphetamine. Cx43 mRNA and protein expression were examined by Northern and Western blots, respectively. We used c-Jun N-terminal kinase (JNK) inhibitor, SP600125, and JNK1 dsRNAi to investigate the signal pathway of amphetamine-induced expression of Cx43. Results: The level of Cx43 protein significantly increased from 4 to 24 h after addition of amphetamine (10 μM). The Cx43 mRNA increased maximally to 4.2-fold at 6 h after addition of amphetamine and returned to the baseline level at 48 h. These increases of Cx43 protein at 24 h were completely attenuated (P

Original languageEnglish
Pages (from-to)98-108
Number of pages11
JournalCardiovascular Research
Volume63
Issue number1
DOIs
Publication statusPublished - Jul 1 2004

Keywords

  • Amphetamine
  • Cardiac myocytes
  • Cell culture
  • Connexin 43
  • Gene expression
  • Signal pathway

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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