Ammonium-dependent sodium uptake in mitochondrion-rich cells of medaka (Oryzias latipes) larvae

Shu Chen Wu, Jiun Lin Horng, Sian Tai Liu, Pung Pung Hwang, Zhi Hong Wen, Chan Shing Lin, Li Yih Lin

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

In this study, a scanning ion-selective electrode technique (SIET) was applied to measure H+, Na+, and NH4+ gradients and apparent fluxes at specific cells on the skin of medaka larvae. Na+ uptake and NH3/NH4+ excretion were detected at most mitochondrion-rich cells (MRCs). H+ probing at MRCs revealed two group of MRCs, i.e., acid-secreting and base-secreting MRCs. Treatment with EIPA (100 μM) blocked 35% of the NH3/NH 4+ secretion and 54% of the Na+ uptake, suggesting that the Na+/H+ exchanger (NHE) is involved in Na+ and NH3/NH4+ transport. Low-Na+ water (4+ (5 mM) acclimation simultaneously increased Na+ uptake and NH 3/NH4+ excretion but decreased or even reversed the H+ gradient at the skin and MRCs. The correlation between NH4+ production and H+ consumption at the skin surface suggests that MRCs excrete nonionic NH3 (base) by an acid-trapping mechanism. Raising the external NH4+ significantly blocked NH3/NH4+ excretion and Na+ uptake. In contrast, raising the acidity of the water (pH 7 to pH 6) enhanced NH3/NH4+ excretion and Na + uptake by MRCs. In situ hybridization and real-time PCR showed that the mRNAs of the Na+/H+ exchanger (slc9a3) and Rhesus glycoproteins (Rhcg1 and Rhbg) were colocalized in MRCs of medaka, and their expressions were induced by low-Na+ acclimation. This study suggests a novel Na+/NH4+ exchange pathway in apical membranes of MRCs, in which a coupled NHE and Rh glycoprotein is involved and the Rh glycoprotein may drive the NHE by generating H+ gradients across apical membranes of MRCs.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume298
Issue number2
DOIs
Publication statusPublished - Feb 2010
Externally publishedYes

Fingerprint

Oryzias
Ammonium Compounds
Larva
Mitochondria
Sodium
Sodium-Hydrogen Antiporter
Glycoproteins
Acclimatization
Skin
Ion-Selective Electrodes
Acids
Membranes
Water
In Situ Hybridization
Real-Time Polymerase Chain Reaction

Keywords

  • Gills
  • Ionocytes
  • Na/H exchanger
  • Osmoregulation
  • Rhesus glycoprotein

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Medicine(all)

Cite this

Ammonium-dependent sodium uptake in mitochondrion-rich cells of medaka (Oryzias latipes) larvae. / Wu, Shu Chen; Horng, Jiun Lin; Liu, Sian Tai; Hwang, Pung Pung; Wen, Zhi Hong; Lin, Chan Shing; Lin, Li Yih.

In: American Journal of Physiology - Cell Physiology, Vol. 298, No. 2, 02.2010.

Research output: Contribution to journalArticle

Wu, Shu Chen ; Horng, Jiun Lin ; Liu, Sian Tai ; Hwang, Pung Pung ; Wen, Zhi Hong ; Lin, Chan Shing ; Lin, Li Yih. / Ammonium-dependent sodium uptake in mitochondrion-rich cells of medaka (Oryzias latipes) larvae. In: American Journal of Physiology - Cell Physiology. 2010 ; Vol. 298, No. 2.
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AU - Wu, Shu Chen

AU - Horng, Jiun Lin

AU - Liu, Sian Tai

AU - Hwang, Pung Pung

AU - Wen, Zhi Hong

AU - Lin, Chan Shing

AU - Lin, Li Yih

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AB - In this study, a scanning ion-selective electrode technique (SIET) was applied to measure H+, Na+, and NH4+ gradients and apparent fluxes at specific cells on the skin of medaka larvae. Na+ uptake and NH3/NH4+ excretion were detected at most mitochondrion-rich cells (MRCs). H+ probing at MRCs revealed two group of MRCs, i.e., acid-secreting and base-secreting MRCs. Treatment with EIPA (100 μM) blocked 35% of the NH3/NH 4+ secretion and 54% of the Na+ uptake, suggesting that the Na+/H+ exchanger (NHE) is involved in Na+ and NH3/NH4+ transport. Low-Na+ water (4+ (5 mM) acclimation simultaneously increased Na+ uptake and NH 3/NH4+ excretion but decreased or even reversed the H+ gradient at the skin and MRCs. The correlation between NH4+ production and H+ consumption at the skin surface suggests that MRCs excrete nonionic NH3 (base) by an acid-trapping mechanism. Raising the external NH4+ significantly blocked NH3/NH4+ excretion and Na+ uptake. In contrast, raising the acidity of the water (pH 7 to pH 6) enhanced NH3/NH4+ excretion and Na + uptake by MRCs. In situ hybridization and real-time PCR showed that the mRNAs of the Na+/H+ exchanger (slc9a3) and Rhesus glycoproteins (Rhcg1 and Rhbg) were colocalized in MRCs of medaka, and their expressions were induced by low-Na+ acclimation. This study suggests a novel Na+/NH4+ exchange pathway in apical membranes of MRCs, in which a coupled NHE and Rh glycoprotein is involved and the Rh glycoprotein may drive the NHE by generating H+ gradients across apical membranes of MRCs.

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