Amentoflavone inhibits hepatocellular carcinoma progression through blockage of ERK/NF-κB activation

Kun Ching Lee, Wei Ting Chen, Yu Chang Liu, Song Shei Lin, Fei Ting Hsu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aim: The aim of the present study was to confirm therapeutic efficacy and find probable mechanism of action of amentoflavone in hepatocellular carcinoma (HCC) in vivo. Materials and Methods: Luciferase reporter vector pGL4.50-transfected SK-Hep1 (SK-Hep1/luc2) tumorbearing mice were treated with vehicle or amentoflavone (100 mg/kg/day by gavage) for 14 days. Tumor growth, amentoflavone toxicity, and extracellular signal-regulated kinase (ERK)/nuclear factor-kappaB (NF-κB) signaling in tumor progression were evaluated with digital caliper, bioluminescence imaging, computed tomography, body weight, pathological examination of liver, and immunohistochemistry staining. Results: Amentoflavone significantly inhibited tumor growth, ERK/NF-κB activation, and expression of tumor progression-associated proteins as compared to vehicle-treated group. In addition, body weight and liver morphology of mice were not influenced by amentoflavone treatment. Conclusion: These results suggest that amentoflavone inhibits HCC progression through suppression of ERK/NF-κB signaling.

Original languageEnglish
Pages (from-to)1097-1103
Number of pages7
JournalIn Vivo
Volume32
Issue number5
DOIs
Publication statusPublished - Sep 1 2018

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Extracellular Signal-Regulated MAP Kinases
Hepatocellular Carcinoma
Chemical activation
Tumors
Liver
Neoplasms
Body Weight
Bioluminescence
Growth
Luciferases
Tomography
Toxicity
amentoflavone
Immunohistochemistry
Staining and Labeling
Imaging techniques
Therapeutics
Proteins

Keywords

  • Amentoflavone
  • Bioluminescence imaging
  • Extracellular signal-regulated kinase
  • Hepatocellular carcinoma
  • Nuclear factor-kappaB

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Amentoflavone inhibits hepatocellular carcinoma progression through blockage of ERK/NF-κB activation. / Lee, Kun Ching; Chen, Wei Ting; Liu, Yu Chang; Lin, Song Shei; Hsu, Fei Ting.

In: In Vivo, Vol. 32, No. 5, 01.09.2018, p. 1097-1103.

Research output: Contribution to journalArticle

Lee, Kun Ching ; Chen, Wei Ting ; Liu, Yu Chang ; Lin, Song Shei ; Hsu, Fei Ting. / Amentoflavone inhibits hepatocellular carcinoma progression through blockage of ERK/NF-κB activation. In: In Vivo. 2018 ; Vol. 32, No. 5. pp. 1097-1103.
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