Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells

Tsung-Hsien Yen, Chia-Ling Hsieh, Tsu-Te Liu, Chih-Sheng Huang, Yen-Chung Chen, Yao-Chen Chuang, Song-Shei Lin, Fei-Ting Hsu

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The goal of the present study was to investigate anticancer effect of amentoflavone on glioblastoma cells in vitro. Our results demonstrated that amentoflavone not only significantly reduced cell viability, nuclear factor-ĸappa B (NF-ĸB) activation, and protein expression of cellular Fas-associated protein with death domain-like interleukin 1 beta-converting enzyme inhibitory protein (C-FLIP) and myeloid cell leukemia 1 (MCL1), but significantly triggered cell accumulation at the sub-G1 phase, loss of mitochondrial membrane potential, and expression of active caspase-3 and -8. In order to verify the effect of NF-ĸB inhibitor on expression of anti-apoptotic proteins, we performed western blotting. We found that the of NF-ĸB inhibitor or amentoflavone markedly diminished protein levels of MCL1 and C-FLIP. Taken all together, our findings show that amentoflavone induces intrinsic and extrinsic apoptosis and inhibits NF-ĸB-modulated anti-apoptotic signaling in U-87 MG cells in vitro.

Original languageEnglish
Pages (from-to)279-285
Number of pages7
JournalIn Vivo
Volume32
Issue number2
DOIs
Publication statusPublished - Feb 25 2018

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