Ameliorate effects of li-fu formula on IL-6-mediated cardiac hypertrophy in hamsters fed with a hyper-cholesterol diet

Bor Show Tzang, Yi Chang Cheng, Chieh His Wu, Wei Wen Kuo, James A. Lin, Hsueh Fang Wang, Fuu Jen Tsai, Chang Hai Tsai, Chih Yang Huang, Tsai Ching Hsu

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Abstract

Hypercholesterolemia diets are considered as major sources to cause cardiac hypertrophy. This study intends to evaluate the effects of Li-Fu formula on cardiac hypertrophy induced by hypercholesterolemia diet. Twenty-four male Golden Syrian hamsters were randomly divided into control, cholesterol and Li-Fu formula groups and fed with different experimental diets for 2 months. Histopathological analysis and western blotting were performed to measure the myocardial architecture, and various cardiac hypertrophy-associated molecules in the excised left ventricle from hamsters. The ratios of whole heart weight/body weight (BW) and left ventricle weight/BW were significantly higher in the cholesterol group but significantly lower in the Li-Fu formula group. The protein levels of both atrial natriuretic peptide and brain natriuretic peptide were significantly increased in the cholesterol group but significantly reduced in the Li-Fu formula group. Additionally, significantly increased interleukin-6, STAT3, MEK5, p-ERK5 and non-cardiomyocyte proliferate signal molecules such as p-MEK and p-ERK, were detected in the cholesterol group but significantly reduced in the Li-Fu formula group. Notably, no significant variations of inflammatory signaling molecules, including p-P38 and p-JNK, were detected in all groups. Our experimental results demonstrated the significant reductions of cardiac hypertrophy and related eccentric hypertrophy signaling, non-cardiomyocyte proliferate signaling in the excised left ventricle of hamsters from the Li-Fu formula. We suggested the protective effects of Li-Fu formula on cardiac hypertrophy that may be useful in prevention or treatment of hypertrophy-associated cardiovascular diseases.

Original languageEnglish
Article number485471
JournalEvidence-based Complementary and Alternative Medicine
Volume2011
DOIs
Publication statusPublished - 2011
Externally publishedYes

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ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Tzang, B. S., Cheng, Y. C., Wu, C. H., Kuo, W. W., Lin, J. A., Wang, H. F., Tsai, F. J., Tsai, C. H., Huang, C. Y., & Hsu, T. C. (2011). Ameliorate effects of li-fu formula on IL-6-mediated cardiac hypertrophy in hamsters fed with a hyper-cholesterol diet. Evidence-based Complementary and Alternative Medicine, 2011, [485471]. https://doi.org/10.1093/ecam/neq066