Amantadine ameliorates dopamine-releasing deficits and behavioral deficits in rats after fluid percussion injury

Eagle Yi Kung Huang, Pi Fen Tsui, Tung Tai Kuo, Jing Tsai, Yu Ching Chou, Hsin I. Ma, Yung Hsiao Chiang, Yuan Hao Chen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aims: To investigate the role of dopamine in cognitive and motor learning skill deficits after a traumatic brain injury (TBI), we investigated dopamine release and behavioral changes at a series of time points after fluid percussion injury, and explored the potential of amantadine hydrochloride as a chronic treatment to provide behavioral recovery. Materials and Methods: In this study, we sequentially investigated dopamine release at the striatum and behavioral changes at 1, 2, 4, 6, and 8 weeks after fluid percussion injury. Rats subjected to 6-Pa cerebral cortical fluid percussion injury were treated by using subcutaneous infusion pumps filled with either saline (sham group) or amantadine hydrochloride, with a releasing rate of 3.6mg/kg/hour for 8 weeks. The dopamine-releasing conditions and metabolism were analyzed sequentially by fast scan cyclic voltammetry (FSCV) and high-pressure liquid chromatography (HPLC). Novel object recognition (NOR) and fixed-speed rotarod (FSRR) behavioral tests were used to determine treatment effects on cognitive and motor deficits after injury. Results: Sequential dopamine-release deficits were revealed in 6-Pa-fluid-percussion cerebral cortical injured animals. The reuptake rate (tau value) of dopamine in injured animals was prolonged, but the tau value became close to the value for the control group after amantadine therapy. Cognitive and motor learning impairments were shown evidenced by the NOR and FSRR behavioral tests after injury. Chronic amantadine therapy reversed dopamine-release deficits, and behavioral impairment after fluid percussion injuries were ameliorated in the rats treated by using amantadine-pumping infusion. Conclusion: Chronic treatment with amantadine hydrochloride can ameliorate dopamine-release deficits as well as cognitive and motor deficits caused by cerebral fluid-percussion injury.

Original languageEnglish
Article numbere86354
JournalPLoS One
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 30 2014

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Percussion
Amantadine
dopamine
Rats
Dopamine
Fluids
rats
Wounds and Injuries
Rotarod Performance Test
Object recognition
Animals
learning
Learning
High pressure liquid chromatography
Therapeutics
Subcutaneous Infusions
Infusion Pumps
therapeutics
Motor Skills
fluids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Huang, E. Y. K., Tsui, P. F., Kuo, T. T., Tsai, J., Chou, Y. C., Ma, H. I., ... Chen, Y. H. (2014). Amantadine ameliorates dopamine-releasing deficits and behavioral deficits in rats after fluid percussion injury. PLoS One, 9(1), [e86354]. https://doi.org/10.1371/journal.pone.0086354

Amantadine ameliorates dopamine-releasing deficits and behavioral deficits in rats after fluid percussion injury. / Huang, Eagle Yi Kung; Tsui, Pi Fen; Kuo, Tung Tai; Tsai, Jing; Chou, Yu Ching; Ma, Hsin I.; Chiang, Yung Hsiao; Chen, Yuan Hao.

In: PLoS One, Vol. 9, No. 1, e86354, 30.01.2014.

Research output: Contribution to journalArticle

Huang, Eagle Yi Kung ; Tsui, Pi Fen ; Kuo, Tung Tai ; Tsai, Jing ; Chou, Yu Ching ; Ma, Hsin I. ; Chiang, Yung Hsiao ; Chen, Yuan Hao. / Amantadine ameliorates dopamine-releasing deficits and behavioral deficits in rats after fluid percussion injury. In: PLoS One. 2014 ; Vol. 9, No. 1.
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