Abstract
Background: The RET proto-oncogene encodes a protein that belongs to the tyrosine kinase growth factor receptor family. Germline point mutations in RET are found in individuals with multiple endocrine neoplasia (MEN) syndromes, and gene rearrangements have been reported in papillary thyroid cancers. We recently identified transcripts of the RET proto-oncogene in human prostate cancer xenografts and prostate cancer cell lines by means of reverse transcription-polymerase chain reaction analyses. The purpose of this study was to investigate Ret protein expression in human prostate tissue. Methods: Ret protein expression was evaluated immunohistochemically in formalin-fixed, paraffin-embedded whole-prostate sections. The prostate specimens were obtained from 30 patients with prostate cancer after radical prostatectomies. Ret protein expression was compared in tumor foci and benign prostatic tissue. Medullary thyroid carcinoma tissue associated with an MEN syndrome and papillary thyroid cancer tissue served as positive controls. Results: Ret appeared to be overexpressed in high-grade (histopathologically advanced) prostatic intraepithelial neoplasia (PIN) and prostate cancer when compared with its expression level in benign prostatic secretory epithelium. In addition, there was an apparent increase in Ret protein expression with decreased cellular differentiation, i.e., increasing Gleason pattern. Conclusion: Expression of the RET proto-oncogene in benign prostatic epithelium, high-grade PIN, and histopathologically advanced prostate cancer suggests that RET may play a role in the growth of both benign and neoplastic prostate epithelial cells.
| Original language | English |
|---|---|
| Pages (from-to) | 519-523 |
| Number of pages | 5 |
| Journal | Journal of the National Cancer Institute |
| Volume | 90 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Apr 1 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Oncology
- Cancer Research
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Altered expression of RET proto-oncogene product in prostatic intraepithelial neoplasia and prostate cancer. / Dawson, Dawn M.; Lawrence, Earl G.; MacLennan, Gregory T.; Amini, Saeid B.; Kung, Hsing Jien; Robinson, Dan; Resnick, Martin I.; Kursh, Elroy D.; Pretlow, Theresa P.; Pretlow, Thomas G.
In: Journal of the National Cancer Institute, Vol. 90, No. 7, 01.04.1998, p. 519-523.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Altered expression of RET proto-oncogene product in prostatic intraepithelial neoplasia and prostate cancer
AU - Dawson, Dawn M.
AU - Lawrence, Earl G.
AU - MacLennan, Gregory T.
AU - Amini, Saeid B.
AU - Kung, Hsing Jien
AU - Robinson, Dan
AU - Resnick, Martin I.
AU - Kursh, Elroy D.
AU - Pretlow, Theresa P.
AU - Pretlow, Thomas G.
PY - 1998/4/1
Y1 - 1998/4/1
N2 - Background: The RET proto-oncogene encodes a protein that belongs to the tyrosine kinase growth factor receptor family. Germline point mutations in RET are found in individuals with multiple endocrine neoplasia (MEN) syndromes, and gene rearrangements have been reported in papillary thyroid cancers. We recently identified transcripts of the RET proto-oncogene in human prostate cancer xenografts and prostate cancer cell lines by means of reverse transcription-polymerase chain reaction analyses. The purpose of this study was to investigate Ret protein expression in human prostate tissue. Methods: Ret protein expression was evaluated immunohistochemically in formalin-fixed, paraffin-embedded whole-prostate sections. The prostate specimens were obtained from 30 patients with prostate cancer after radical prostatectomies. Ret protein expression was compared in tumor foci and benign prostatic tissue. Medullary thyroid carcinoma tissue associated with an MEN syndrome and papillary thyroid cancer tissue served as positive controls. Results: Ret appeared to be overexpressed in high-grade (histopathologically advanced) prostatic intraepithelial neoplasia (PIN) and prostate cancer when compared with its expression level in benign prostatic secretory epithelium. In addition, there was an apparent increase in Ret protein expression with decreased cellular differentiation, i.e., increasing Gleason pattern. Conclusion: Expression of the RET proto-oncogene in benign prostatic epithelium, high-grade PIN, and histopathologically advanced prostate cancer suggests that RET may play a role in the growth of both benign and neoplastic prostate epithelial cells.
AB - Background: The RET proto-oncogene encodes a protein that belongs to the tyrosine kinase growth factor receptor family. Germline point mutations in RET are found in individuals with multiple endocrine neoplasia (MEN) syndromes, and gene rearrangements have been reported in papillary thyroid cancers. We recently identified transcripts of the RET proto-oncogene in human prostate cancer xenografts and prostate cancer cell lines by means of reverse transcription-polymerase chain reaction analyses. The purpose of this study was to investigate Ret protein expression in human prostate tissue. Methods: Ret protein expression was evaluated immunohistochemically in formalin-fixed, paraffin-embedded whole-prostate sections. The prostate specimens were obtained from 30 patients with prostate cancer after radical prostatectomies. Ret protein expression was compared in tumor foci and benign prostatic tissue. Medullary thyroid carcinoma tissue associated with an MEN syndrome and papillary thyroid cancer tissue served as positive controls. Results: Ret appeared to be overexpressed in high-grade (histopathologically advanced) prostatic intraepithelial neoplasia (PIN) and prostate cancer when compared with its expression level in benign prostatic secretory epithelium. In addition, there was an apparent increase in Ret protein expression with decreased cellular differentiation, i.e., increasing Gleason pattern. Conclusion: Expression of the RET proto-oncogene in benign prostatic epithelium, high-grade PIN, and histopathologically advanced prostate cancer suggests that RET may play a role in the growth of both benign and neoplastic prostate epithelial cells.
UR - http://www.scopus.com/inward/record.url?scp=0032054021&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032054021&partnerID=8YFLogxK
U2 - 10.1093/jnci/90.7.519
DO - 10.1093/jnci/90.7.519
M3 - Article
C2 - 9539247
AN - SCOPUS:0032054021
VL - 90
SP - 519
EP - 523
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
SN - 0027-8874
IS - 7
ER -