Alterative effects of an oral alginate extract on experimental rabbit osteoarthritis

Hsien Tseng Lu, Ming-Shium Hsieh, Chao Wen Cheng, Li Fan Yao, Tsuey Ying Hsu, Jai Lan, Kwang Yoon Kim, Suk Jung Oh, Yung Hsiang Chang, Chian Her Lee, Yung Feng Lin, Chien Ho Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Osteoarthritis (OA) is a common joint disease that causes disabilities in elderly. However, few agents with high efficacy and low side effects have been developed to treat OA. In this study, we evaluated the effects of the alginate extract named CTX in OA cell and rabbit models. Results: CTX was formulated by hydrolyzing sodium alginate polymers with alginate lyase and then mixing with pectin. HPLC was used to analyze the CTX content. Human chondrosarcoma SW1353 cells treated with interleukin-1β were used as OA model cells to investigate the effects of CTX on chondrocyte inflammation and anabolism. CTX at concentrations up to 1000 μg/ml exerted low cytotoxicity. It inhibited the gene expression of proinflammatory matrix metalloproteinases (MMPs) including MMP1, MMP3 and MMP13 in a dose-dependent manner and increased the mRNA level of aggrecan, the major proteoglycan in articular cartilage, at 1000 μg/ml. Thirteen-week-old New Zealand White rabbits underwent a surgical anterior cruciate ligament transection and were orally treated with normal saline, glucosamine or CTX for up to 7 weeks. Examinations of the rabbit femur and tibia samples demonstrated that the rabbits taking oral CTX at a dosage of 30 mg/kg/day suffered lesser degrees of articular stiffness and histological cartilage damage than the control rabbits. Conclusions: The gene expression profiles in the cell and the examinations done on the rabbit cartilage suggest that the alginate extract CTX is a pharmaco-therapeutic agent applicable for OA therapy.

Original languageEnglish
Article number64
JournalJournal of Biomedical Science
Volume22
Issue number1
DOIs
Publication statusPublished - Aug 4 2015

Fingerprint

Cartilage
Osteoarthritis
Rabbits
Gene expression
Aggrecans
Glucosamine
Ligaments
Proteoglycans
Cytotoxicity
Matrix Metalloproteinases
Interleukin-1
Polymers
Stiffness
Messenger RNA
Chondrosarcoma
Joint Diseases
Anterior Cruciate Ligament
Articular Cartilage
Chondrocytes
Tibia

Keywords

  • Aggrecan
  • Anterior cruciate ligament transection
  • CTX
  • Interleukin-1β
  • Matrix metalloproteinases
  • Osteoarthritis

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism
  • Pharmacology (medical)

Cite this

Alterative effects of an oral alginate extract on experimental rabbit osteoarthritis. / Lu, Hsien Tseng; Hsieh, Ming-Shium; Cheng, Chao Wen; Yao, Li Fan; Hsu, Tsuey Ying; Lan, Jai; Kim, Kwang Yoon; Oh, Suk Jung; Chang, Yung Hsiang; Lee, Chian Her; Lin, Yung Feng; Chen, Chien Ho.

In: Journal of Biomedical Science, Vol. 22, No. 1, 64, 04.08.2015.

Research output: Contribution to journalArticle

Lu, Hsien Tseng ; Hsieh, Ming-Shium ; Cheng, Chao Wen ; Yao, Li Fan ; Hsu, Tsuey Ying ; Lan, Jai ; Kim, Kwang Yoon ; Oh, Suk Jung ; Chang, Yung Hsiang ; Lee, Chian Her ; Lin, Yung Feng ; Chen, Chien Ho. / Alterative effects of an oral alginate extract on experimental rabbit osteoarthritis. In: Journal of Biomedical Science. 2015 ; Vol. 22, No. 1.
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abstract = "Background: Osteoarthritis (OA) is a common joint disease that causes disabilities in elderly. However, few agents with high efficacy and low side effects have been developed to treat OA. In this study, we evaluated the effects of the alginate extract named CTX in OA cell and rabbit models. Results: CTX was formulated by hydrolyzing sodium alginate polymers with alginate lyase and then mixing with pectin. HPLC was used to analyze the CTX content. Human chondrosarcoma SW1353 cells treated with interleukin-1β were used as OA model cells to investigate the effects of CTX on chondrocyte inflammation and anabolism. CTX at concentrations up to 1000 μg/ml exerted low cytotoxicity. It inhibited the gene expression of proinflammatory matrix metalloproteinases (MMPs) including MMP1, MMP3 and MMP13 in a dose-dependent manner and increased the mRNA level of aggrecan, the major proteoglycan in articular cartilage, at 1000 μg/ml. Thirteen-week-old New Zealand White rabbits underwent a surgical anterior cruciate ligament transection and were orally treated with normal saline, glucosamine or CTX for up to 7 weeks. Examinations of the rabbit femur and tibia samples demonstrated that the rabbits taking oral CTX at a dosage of 30 mg/kg/day suffered lesser degrees of articular stiffness and histological cartilage damage than the control rabbits. Conclusions: The gene expression profiles in the cell and the examinations done on the rabbit cartilage suggest that the alginate extract CTX is a pharmaco-therapeutic agent applicable for OA therapy.",
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AU - Hsu, Tsuey Ying

AU - Lan, Jai

AU - Kim, Kwang Yoon

AU - Oh, Suk Jung

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AU - Lee, Chian Her

AU - Lin, Yung Feng

AU - Chen, Chien Ho

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AB - Background: Osteoarthritis (OA) is a common joint disease that causes disabilities in elderly. However, few agents with high efficacy and low side effects have been developed to treat OA. In this study, we evaluated the effects of the alginate extract named CTX in OA cell and rabbit models. Results: CTX was formulated by hydrolyzing sodium alginate polymers with alginate lyase and then mixing with pectin. HPLC was used to analyze the CTX content. Human chondrosarcoma SW1353 cells treated with interleukin-1β were used as OA model cells to investigate the effects of CTX on chondrocyte inflammation and anabolism. CTX at concentrations up to 1000 μg/ml exerted low cytotoxicity. It inhibited the gene expression of proinflammatory matrix metalloproteinases (MMPs) including MMP1, MMP3 and MMP13 in a dose-dependent manner and increased the mRNA level of aggrecan, the major proteoglycan in articular cartilage, at 1000 μg/ml. Thirteen-week-old New Zealand White rabbits underwent a surgical anterior cruciate ligament transection and were orally treated with normal saline, glucosamine or CTX for up to 7 weeks. Examinations of the rabbit femur and tibia samples demonstrated that the rabbits taking oral CTX at a dosage of 30 mg/kg/day suffered lesser degrees of articular stiffness and histological cartilage damage than the control rabbits. Conclusions: The gene expression profiles in the cell and the examinations done on the rabbit cartilage suggest that the alginate extract CTX is a pharmaco-therapeutic agent applicable for OA therapy.

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