Alpinate oxyphyllae fructus (Alpinia oxyphylla miq) extracts inhibit angiotensin-II induced cardiac apoptosis in H9c2 cardiomyoblast cells

Yung Ming Chang, Chuan Te Tsai, Chiun Chuang Roger Wang, Yueh Sheng Chen, Yueh Min Lin, Chia Hua Kuo, Bor Show Tzang, Ray Jade Chen, Fuu Jen Tsai, Chih Yang Huang

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13 Citations (Scopus)

Abstract

Angiotensin II (Ang II) is a risk factor for cardiovascular disease. We used a traditional Chinese medicine, alpinate oxyphyllae fructus (AOF), to evaluate its effect on Ang II-induced cardiac apoptosis and mitochondrial dysfunction. Ang II-treated H9c2 cells were administered AOF of 20-100μg/mL concentrations. Ang II significantly increased TUNEL-positive nuclei in the H9c2 cells, effect was inhibited by AOF administration in both pre-treated and post-treated H9c2 cells. Caspases 9 and 3 activities were increased by Ang II and downregulated by AOF administration, especially in pre-treatment. AOF treatment reversed Ang II-induced mitochondria membrane potential instability in H9c2 cells as observed by JC-1 stain assay. Furthermore, proapoptotic proteins Bad and cytochrome c increased and decreased respectively under AOF administration. The levels of p-Bad anti-apoptotic protein were significantly increased after AOF treatment. This study indicates that mitochondrial dependent apoptosis induced by Ang II.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number2
DOIs
Publication statusPublished - 2013

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Keywords

  • Alpinate oxyphyllae fructus
  • Angiotensin II
  • Apoptosis
  • Cardiomyoblast cell
  • H9c2 cell

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

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