Alpha coefficient of dose-response for chromosome translocations measured by FISH in human lymphocytes exposed to chronic 60Co gamma rays at body temperature

W. A. Hsieh, W. Deng, W. P. Chang, N. Galvan, C. L. Owens, D. P. Morrison, K. L. Gale, J. N. Lucas

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Purpose: To measure the alpha coefficient, the initial slope of the translocation dose-response curve, for 60Co γ-rays in human lymphocytes. Materials and methods: Human lymphocytes were exposed to 0, 0.32, 0.62 and 0.92 Gy of chronic 60Co γ-rays under conditions that reduce the metabolic stress to the cells. Chromosome translocation frequencies were measured using fluorescence in situ hybridization with a whole-chromosome probe cocktail specific for chromosomes 1, 2, 4 (orange) and 3, 5, 6 (green). Results: A total of 72 383 metaphases were analysed (33 429 in exposed cells) in two individuals. The shape of the dose-response curves for translocations was linear, and alpha coefficient was measured as 0.024 ± 0.002 translocations per cell per Gy for the combined data for two 24 year old male donors. Conclusion: The alpha coefficients measured after chronic exposure were in good agreement with that reported in the literature for acute, low-dose exposure of human lymphocytes to 60Co γ-rays.

Original languageEnglish
Pages (from-to)435-439
Number of pages5
JournalInternational Journal of Radiation Biology
Volume75
Issue number4
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

body temperature
Lymphocytes
lymphocytes
chromosomes
Gamma Rays
chromosome translocation
Chromosomes
Body Temperature
Gamma rays
dose response
gamma radiation
rays
gamma rays
dosage
coefficients
cells
Physiological Stress
Chromosomes, Human, Pair 2
Chromosomes, Human, Pair 1
curves

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Alpha coefficient of dose-response for chromosome translocations measured by FISH in human lymphocytes exposed to chronic 60Co gamma rays at body temperature. / Hsieh, W. A.; Deng, W.; Chang, W. P.; Galvan, N.; Owens, C. L.; Morrison, D. P.; Gale, K. L.; Lucas, J. N.

In: International Journal of Radiation Biology, Vol. 75, No. 4, 1999, p. 435-439.

Research output: Contribution to journalArticle

Hsieh, W. A. ; Deng, W. ; Chang, W. P. ; Galvan, N. ; Owens, C. L. ; Morrison, D. P. ; Gale, K. L. ; Lucas, J. N. / Alpha coefficient of dose-response for chromosome translocations measured by FISH in human lymphocytes exposed to chronic 60Co gamma rays at body temperature. In: International Journal of Radiation Biology. 1999 ; Vol. 75, No. 4. pp. 435-439.
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AU - Hsieh, W. A.

AU - Deng, W.

AU - Chang, W. P.

AU - Galvan, N.

AU - Owens, C. L.

AU - Morrison, D. P.

AU - Gale, K. L.

AU - Lucas, J. N.

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