Airway extravasation induced by increasing airway temperature in ovalbumin-sensitized rats

Chun-Chun Hsu, Reyno J. Tapia, Lu-Yuan Lee

Research output: Contribution to journalArticle

Abstract

This study was carried out to determine whether hyperventilation of humidified warm air (HWA) induced airway extravasation in ovalbumin (Ova)-sensitized rats. Our results showed: (1) After isocapnic hyperventilation with HWA for 2min, tracheal temperature (Ttr) was increased to 40.3°C, and the Evans blue contents in major airways and lung tissue were elevated to 651% and 707%, respectively, of that after hyperventilation with humidified room air in Ova-sensitized rats; this striking effect of HWA was absent in control rats. (2) The HWA-induced increase in Evans blue content in sensitized rats was completely prevented by a pretreatment with either L-732138, a selective antagonist of neurokinin type 1 (NK-1) receptor, or formoterol, a selective agonist of β2 adrenoceptor. This study demonstrated that an increase in airway temperature induced protein extravasation in the major airways and lung tissue of sensitized rats, and an activation of the NK-1 receptor by tachykinins released from bronchopulmonary C-fiber nerve endings was primarily responsible. © 2015.
Original languageEnglish
Pages (from-to)46-49
Number of pages4
JournalRespiratory Physiology and Neurobiology
Volume212-214
DOIs
Publication statusPublished - 2015
Externally publishedYes

Fingerprint

Ovalbumin
Air
Hyperventilation
Temperature
Evans Blue
3,5-bis(trifluoromethyl)benzyl N-acetyltryptophan
Neurokinin-1 Receptor Antagonists
Neurokinin-1 Receptors
Tachykinins
Lung
Unmyelinated Nerve Fibers
Nerve Endings
Adrenergic Receptors
Proteins

Keywords

  • Asthma
  • Bronchoconstriction
  • C-fiber
  • Tachykinins
  • Vagus
  • Evans blue
  • formoterol
  • ovalbumin
  • tachykinin
  • airway
  • animal experiment
  • animal model
  • animal tissue
  • Article
  • bronchoconstriction
  • controlled study
  • extravasation
  • hyperventilation
  • lung parenchyma
  • male
  • nonhuman
  • priority journal
  • rat
  • temperature measurement

Cite this

Airway extravasation induced by increasing airway temperature in ovalbumin-sensitized rats. / Hsu, Chun-Chun; Tapia, Reyno J.; Lee, Lu-Yuan.

In: Respiratory Physiology and Neurobiology, Vol. 212-214, 2015, p. 46-49.

Research output: Contribution to journalArticle

@article{615fe851d3dc4ab98edb4c362252603b,
title = "Airway extravasation induced by increasing airway temperature in ovalbumin-sensitized rats",
abstract = "This study was carried out to determine whether hyperventilation of humidified warm air (HWA) induced airway extravasation in ovalbumin (Ova)-sensitized rats. Our results showed: (1) After isocapnic hyperventilation with HWA for 2min, tracheal temperature (Ttr) was increased to 40.3°C, and the Evans blue contents in major airways and lung tissue were elevated to 651{\%} and 707{\%}, respectively, of that after hyperventilation with humidified room air in Ova-sensitized rats; this striking effect of HWA was absent in control rats. (2) The HWA-induced increase in Evans blue content in sensitized rats was completely prevented by a pretreatment with either L-732138, a selective antagonist of neurokinin type 1 (NK-1) receptor, or formoterol, a selective agonist of β2 adrenoceptor. This study demonstrated that an increase in airway temperature induced protein extravasation in the major airways and lung tissue of sensitized rats, and an activation of the NK-1 receptor by tachykinins released from bronchopulmonary C-fiber nerve endings was primarily responsible. {\circledC} 2015.",
keywords = "Asthma, Bronchoconstriction, C-fiber, Tachykinins, Vagus, Evans blue, formoterol, ovalbumin, tachykinin, airway, animal experiment, animal model, animal tissue, Article, bronchoconstriction, controlled study, extravasation, hyperventilation, lung parenchyma, male, nonhuman, priority journal, rat, temperature measurement",
author = "Chun-Chun Hsu and Tapia, {Reyno J.} and Lu-Yuan Lee",
note = "Export Date: 11 May 2016 CODEN: RPNEA 通訊地址: Lee, L.-Y.; Department of Physiology, University of Kentucky Medical Center, 800 Rose St., MS511A, United States 化學物質/CAS: Evans blue, 314-13-6; formoterol, 73573-87-2; ovalbumin, 77466-29-6 參考文獻: Baluk, P., Bolton, P., Hirata, A., Thurston, G., McDonald, D.M., Endothelial gaps and adherent leukocytes in allergen-induced early- and late-phase plasma leakage in rat airways (1998) Am. J. Pathol., 152, pp. 1463-1476; Baluk, P., McDonald, D.M., The beta 2-adrenergic receptor agonist formoterol reduces microvascular leakage by inhibiting endothelial gap formation (1994) Am. J. Physiol., 266, pp. L461-L468; Evans, T.W., Rogers, D.F., Aursudkij, B., Chung, K.F., Barnes, P.J., Inflammatory mediators involved in antigen-induced airway microvascular leakage in guinea pigs (1988) Am. Rev. Respir. Dis., 138, pp. 395-399; Fischer, A., McGregor, G.P., Saria, A., Philippin, B., Kummer, W., Induction of tachykinin gene and peptide expression in guinea pig nodose primary afferent neurons by allergic airway inflammation (1996) J. Clin. Invest., 98, pp. 2284-2291; Groshaus, H.E., Manocha, S., Walley, K.R., Russell, J.A., Mechanisms of beta-receptor stimulation-induced improvement of acute lung injury and pulmonary edema (2004) Crit. Care (London, England), 8, pp. 234-242; Hayes, D., Collins, P.B., Khosravi, M., Lin, R.L., Lee, L.Y., Bronchoconstriction triggered by breathing hot humid air in patients with asthma: role of cholinergic reflex (2012) Am. J. Respir. Crit. Care Med., 185, pp. 1190-1196; Hsu, C.C., Lin, R.L., Lin, Y.S., Lee, L.Y., Bronchoconstriction induced by increasing airway temperature in ovalbumin-sensitized rats: role of tachykinins (2013) J. Appl. Physiol. (Bethesda, Md.: 1985), 115, pp. 688-696; Lin, Y.J., Lee, L.Y., Hypersensitivity of bronchopulmonary C-fibers induced by an increase in airway temperature in ovalbumin (Ova)-sensitized Brown Norway rats (2013) FASEB J., 27. , 930.19 (Abstract); McDonald, D.M., Neurogenic inflammation in the rat trachea. I. Changes in venules, leucocytes and epithelial cells (1988) J. Neurocytol., 17, pp. 583-603; Myers, A.C., Kajekar, R., Undem, B.J., Allergic inflammation-induced neuropeptide production in rapidly adapting afferent nerves in guinea pig airways (2002) Am. J. Physiol. Lung Cell. Mol. Physiol., 282, pp. L775-L781; Steinhoff, M.S., von Mentzer, B., Geppetti, P., Pothoulakis, C., Bunnett, N.W., Tachykinins and their receptors: contributions to physiological control and the mechanisms of disease (2014) Physiol. Rev., 94, pp. 265-301",
year = "2015",
doi = "10.1016/j.resp.2015.04.002",
language = "English",
volume = "212-214",
pages = "46--49",
journal = "Respiratory Physiology and Neurobiology",
issn = "1569-9048",
publisher = "Elsevier Science Publishers B.V.",

}

TY - JOUR

T1 - Airway extravasation induced by increasing airway temperature in ovalbumin-sensitized rats

AU - Hsu, Chun-Chun

AU - Tapia, Reyno J.

AU - Lee, Lu-Yuan

N1 - Export Date: 11 May 2016 CODEN: RPNEA 通訊地址: Lee, L.-Y.; Department of Physiology, University of Kentucky Medical Center, 800 Rose St., MS511A, United States 化學物質/CAS: Evans blue, 314-13-6; formoterol, 73573-87-2; ovalbumin, 77466-29-6 參考文獻: Baluk, P., Bolton, P., Hirata, A., Thurston, G., McDonald, D.M., Endothelial gaps and adherent leukocytes in allergen-induced early- and late-phase plasma leakage in rat airways (1998) Am. J. Pathol., 152, pp. 1463-1476; Baluk, P., McDonald, D.M., The beta 2-adrenergic receptor agonist formoterol reduces microvascular leakage by inhibiting endothelial gap formation (1994) Am. J. Physiol., 266, pp. L461-L468; Evans, T.W., Rogers, D.F., Aursudkij, B., Chung, K.F., Barnes, P.J., Inflammatory mediators involved in antigen-induced airway microvascular leakage in guinea pigs (1988) Am. Rev. Respir. Dis., 138, pp. 395-399; Fischer, A., McGregor, G.P., Saria, A., Philippin, B., Kummer, W., Induction of tachykinin gene and peptide expression in guinea pig nodose primary afferent neurons by allergic airway inflammation (1996) J. Clin. Invest., 98, pp. 2284-2291; Groshaus, H.E., Manocha, S., Walley, K.R., Russell, J.A., Mechanisms of beta-receptor stimulation-induced improvement of acute lung injury and pulmonary edema (2004) Crit. Care (London, England), 8, pp. 234-242; Hayes, D., Collins, P.B., Khosravi, M., Lin, R.L., Lee, L.Y., Bronchoconstriction triggered by breathing hot humid air in patients with asthma: role of cholinergic reflex (2012) Am. J. Respir. Crit. Care Med., 185, pp. 1190-1196; Hsu, C.C., Lin, R.L., Lin, Y.S., Lee, L.Y., Bronchoconstriction induced by increasing airway temperature in ovalbumin-sensitized rats: role of tachykinins (2013) J. Appl. Physiol. (Bethesda, Md.: 1985), 115, pp. 688-696; Lin, Y.J., Lee, L.Y., Hypersensitivity of bronchopulmonary C-fibers induced by an increase in airway temperature in ovalbumin (Ova)-sensitized Brown Norway rats (2013) FASEB J., 27. , 930.19 (Abstract); McDonald, D.M., Neurogenic inflammation in the rat trachea. I. Changes in venules, leucocytes and epithelial cells (1988) J. Neurocytol., 17, pp. 583-603; Myers, A.C., Kajekar, R., Undem, B.J., Allergic inflammation-induced neuropeptide production in rapidly adapting afferent nerves in guinea pig airways (2002) Am. J. Physiol. Lung Cell. Mol. Physiol., 282, pp. L775-L781; Steinhoff, M.S., von Mentzer, B., Geppetti, P., Pothoulakis, C., Bunnett, N.W., Tachykinins and their receptors: contributions to physiological control and the mechanisms of disease (2014) Physiol. Rev., 94, pp. 265-301

PY - 2015

Y1 - 2015

N2 - This study was carried out to determine whether hyperventilation of humidified warm air (HWA) induced airway extravasation in ovalbumin (Ova)-sensitized rats. Our results showed: (1) After isocapnic hyperventilation with HWA for 2min, tracheal temperature (Ttr) was increased to 40.3°C, and the Evans blue contents in major airways and lung tissue were elevated to 651% and 707%, respectively, of that after hyperventilation with humidified room air in Ova-sensitized rats; this striking effect of HWA was absent in control rats. (2) The HWA-induced increase in Evans blue content in sensitized rats was completely prevented by a pretreatment with either L-732138, a selective antagonist of neurokinin type 1 (NK-1) receptor, or formoterol, a selective agonist of β2 adrenoceptor. This study demonstrated that an increase in airway temperature induced protein extravasation in the major airways and lung tissue of sensitized rats, and an activation of the NK-1 receptor by tachykinins released from bronchopulmonary C-fiber nerve endings was primarily responsible. © 2015.

AB - This study was carried out to determine whether hyperventilation of humidified warm air (HWA) induced airway extravasation in ovalbumin (Ova)-sensitized rats. Our results showed: (1) After isocapnic hyperventilation with HWA for 2min, tracheal temperature (Ttr) was increased to 40.3°C, and the Evans blue contents in major airways and lung tissue were elevated to 651% and 707%, respectively, of that after hyperventilation with humidified room air in Ova-sensitized rats; this striking effect of HWA was absent in control rats. (2) The HWA-induced increase in Evans blue content in sensitized rats was completely prevented by a pretreatment with either L-732138, a selective antagonist of neurokinin type 1 (NK-1) receptor, or formoterol, a selective agonist of β2 adrenoceptor. This study demonstrated that an increase in airway temperature induced protein extravasation in the major airways and lung tissue of sensitized rats, and an activation of the NK-1 receptor by tachykinins released from bronchopulmonary C-fiber nerve endings was primarily responsible. © 2015.

KW - Asthma

KW - Bronchoconstriction

KW - C-fiber

KW - Tachykinins

KW - Vagus

KW - Evans blue

KW - formoterol

KW - ovalbumin

KW - tachykinin

KW - airway

KW - animal experiment

KW - animal model

KW - animal tissue

KW - Article

KW - bronchoconstriction

KW - controlled study

KW - extravasation

KW - hyperventilation

KW - lung parenchyma

KW - male

KW - nonhuman

KW - priority journal

KW - rat

KW - temperature measurement

U2 - 10.1016/j.resp.2015.04.002

DO - 10.1016/j.resp.2015.04.002

M3 - Article

VL - 212-214

SP - 46

EP - 49

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

ER -