Agmatine Induces Rat Prostate Relaxation through Activation of Peripheral Imidazoline I2-Receptors

Liang Ming Lee, Chia Ho Lin, Hsien Hui Chung, Juei Tang Cheng, I. Hung Chen, Yat Ching Tong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives: The effect of agmatine on prostate contractility as well as the roles of imidazoline receptors and potassium channels in this action were studied using isolated Wistar rat prostate tissue. Methods: Rat prostate strips were pre-contracted with 1 μmol/L phenylephrine or 50 mmol/L KCl. The relaxation response to agmatine (1-100 μmol/L) was measured. The effects of imidazoline receptor blockers: efaroxan, BU224, KU14R; ATP-sensitive K+ channels (KATP) channel inhibitor: glibenclamide; cyclic AMP (cAMP) phosphodiesterase inhibitor: IBMX; or protein kinase A (PKA) inhibitor: H-89 on the agmatine-induced relaxation were studied. Results: Agmatine produced relaxation in prostate strips pre-contracted with phenylephrine or KCl in a dose-dependent manner. This relaxation was significantly reduced by BU224, a selective I2 imidazoline receptor (IR) blocker, but not by I1 or I3 IR blockers (efaroxan, KU14R respectively). Moreover, the agmatine-induced relaxation was attenuated by glibenclamide and H-89, but enhanced by IBMX. Conclusion: The results suggest that agmatine causes rat prostate relaxation by activation of the I2 IR, which opens KATP channels through cAMP/PKA pathway.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalLUTS: Lower Urinary Tract Symptoms
Volume5
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Agmatine
Prostate
efaroxan
Imidazoline Receptors
1-Methyl-3-isobutylxanthine
KATP Channels
Glyburide
Phenylephrine
Cyclic AMP-Dependent Protein Kinases
Cyclic AMP
Adenylate Kinase
Phosphodiesterase Inhibitors
Potassium Channels
Protein Kinase Inhibitors
imidazoline receptor 2
Wistar Rats
Adenosine Triphosphate

Keywords

  • Adenosine-5'-triphosphate-sensitive K channel
  • Agmatine
  • Imidazoline receptor
  • Prostate

ASJC Scopus subject areas

  • Urology
  • Neurology

Cite this

Agmatine Induces Rat Prostate Relaxation through Activation of Peripheral Imidazoline I2-Receptors. / Lee, Liang Ming; Lin, Chia Ho; Chung, Hsien Hui; Cheng, Juei Tang; Chen, I. Hung; Tong, Yat Ching.

In: LUTS: Lower Urinary Tract Symptoms, Vol. 5, No. 1, 01.2013, p. 39-43.

Research output: Contribution to journalArticle

Lee, Liang Ming ; Lin, Chia Ho ; Chung, Hsien Hui ; Cheng, Juei Tang ; Chen, I. Hung ; Tong, Yat Ching. / Agmatine Induces Rat Prostate Relaxation through Activation of Peripheral Imidazoline I2-Receptors. In: LUTS: Lower Urinary Tract Symptoms. 2013 ; Vol. 5, No. 1. pp. 39-43.
@article{4fa7bc2c8aba49be9db9e393ae1d5ef0,
title = "Agmatine Induces Rat Prostate Relaxation through Activation of Peripheral Imidazoline I2-Receptors",
abstract = "Objectives: The effect of agmatine on prostate contractility as well as the roles of imidazoline receptors and potassium channels in this action were studied using isolated Wistar rat prostate tissue. Methods: Rat prostate strips were pre-contracted with 1 μmol/L phenylephrine or 50 mmol/L KCl. The relaxation response to agmatine (1-100 μmol/L) was measured. The effects of imidazoline receptor blockers: efaroxan, BU224, KU14R; ATP-sensitive K+ channels (KATP) channel inhibitor: glibenclamide; cyclic AMP (cAMP) phosphodiesterase inhibitor: IBMX; or protein kinase A (PKA) inhibitor: H-89 on the agmatine-induced relaxation were studied. Results: Agmatine produced relaxation in prostate strips pre-contracted with phenylephrine or KCl in a dose-dependent manner. This relaxation was significantly reduced by BU224, a selective I2 imidazoline receptor (IR) blocker, but not by I1 or I3 IR blockers (efaroxan, KU14R respectively). Moreover, the agmatine-induced relaxation was attenuated by glibenclamide and H-89, but enhanced by IBMX. Conclusion: The results suggest that agmatine causes rat prostate relaxation by activation of the I2 IR, which opens KATP channels through cAMP/PKA pathway.",
keywords = "Adenosine-5'-triphosphate-sensitive K channel, Agmatine, Imidazoline receptor, Prostate",
author = "Lee, {Liang Ming} and Lin, {Chia Ho} and Chung, {Hsien Hui} and Cheng, {Juei Tang} and Chen, {I. Hung} and Tong, {Yat Ching}",
year = "2013",
month = "1",
doi = "10.1111/j.1757-5672.2012.00158.x",
language = "English",
volume = "5",
pages = "39--43",
journal = "LUTS: Lower Urinary Tract Symptoms",
issn = "1757-5664",
publisher = "Wiley Blackwell",
number = "1",

}

TY - JOUR

T1 - Agmatine Induces Rat Prostate Relaxation through Activation of Peripheral Imidazoline I2-Receptors

AU - Lee, Liang Ming

AU - Lin, Chia Ho

AU - Chung, Hsien Hui

AU - Cheng, Juei Tang

AU - Chen, I. Hung

AU - Tong, Yat Ching

PY - 2013/1

Y1 - 2013/1

N2 - Objectives: The effect of agmatine on prostate contractility as well as the roles of imidazoline receptors and potassium channels in this action were studied using isolated Wistar rat prostate tissue. Methods: Rat prostate strips were pre-contracted with 1 μmol/L phenylephrine or 50 mmol/L KCl. The relaxation response to agmatine (1-100 μmol/L) was measured. The effects of imidazoline receptor blockers: efaroxan, BU224, KU14R; ATP-sensitive K+ channels (KATP) channel inhibitor: glibenclamide; cyclic AMP (cAMP) phosphodiesterase inhibitor: IBMX; or protein kinase A (PKA) inhibitor: H-89 on the agmatine-induced relaxation were studied. Results: Agmatine produced relaxation in prostate strips pre-contracted with phenylephrine or KCl in a dose-dependent manner. This relaxation was significantly reduced by BU224, a selective I2 imidazoline receptor (IR) blocker, but not by I1 or I3 IR blockers (efaroxan, KU14R respectively). Moreover, the agmatine-induced relaxation was attenuated by glibenclamide and H-89, but enhanced by IBMX. Conclusion: The results suggest that agmatine causes rat prostate relaxation by activation of the I2 IR, which opens KATP channels through cAMP/PKA pathway.

AB - Objectives: The effect of agmatine on prostate contractility as well as the roles of imidazoline receptors and potassium channels in this action were studied using isolated Wistar rat prostate tissue. Methods: Rat prostate strips were pre-contracted with 1 μmol/L phenylephrine or 50 mmol/L KCl. The relaxation response to agmatine (1-100 μmol/L) was measured. The effects of imidazoline receptor blockers: efaroxan, BU224, KU14R; ATP-sensitive K+ channels (KATP) channel inhibitor: glibenclamide; cyclic AMP (cAMP) phosphodiesterase inhibitor: IBMX; or protein kinase A (PKA) inhibitor: H-89 on the agmatine-induced relaxation were studied. Results: Agmatine produced relaxation in prostate strips pre-contracted with phenylephrine or KCl in a dose-dependent manner. This relaxation was significantly reduced by BU224, a selective I2 imidazoline receptor (IR) blocker, but not by I1 or I3 IR blockers (efaroxan, KU14R respectively). Moreover, the agmatine-induced relaxation was attenuated by glibenclamide and H-89, but enhanced by IBMX. Conclusion: The results suggest that agmatine causes rat prostate relaxation by activation of the I2 IR, which opens KATP channels through cAMP/PKA pathway.

KW - Adenosine-5'-triphosphate-sensitive K channel

KW - Agmatine

KW - Imidazoline receptor

KW - Prostate

UR - http://www.scopus.com/inward/record.url?scp=84872719258&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872719258&partnerID=8YFLogxK

U2 - 10.1111/j.1757-5672.2012.00158.x

DO - 10.1111/j.1757-5672.2012.00158.x

M3 - Article

AN - SCOPUS:84872719258

VL - 5

SP - 39

EP - 43

JO - LUTS: Lower Urinary Tract Symptoms

JF - LUTS: Lower Urinary Tract Symptoms

SN - 1757-5664

IS - 1

ER -