Agmatine Induces Rat Prostate Relaxation through Activation of Peripheral Imidazoline I2-Receptors

Liang Ming Lee, Chia Ho Lin, Hsien Hui Chung, Juei Tang Cheng, I. Hung Chen, Yat Ching Tong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives: The effect of agmatine on prostate contractility as well as the roles of imidazoline receptors and potassium channels in this action were studied using isolated Wistar rat prostate tissue. Methods: Rat prostate strips were pre-contracted with 1 μmol/L phenylephrine or 50 mmol/L KCl. The relaxation response to agmatine (1-100 μmol/L) was measured. The effects of imidazoline receptor blockers: efaroxan, BU224, KU14R; ATP-sensitive K+ channels (KATP) channel inhibitor: glibenclamide; cyclic AMP (cAMP) phosphodiesterase inhibitor: IBMX; or protein kinase A (PKA) inhibitor: H-89 on the agmatine-induced relaxation were studied. Results: Agmatine produced relaxation in prostate strips pre-contracted with phenylephrine or KCl in a dose-dependent manner. This relaxation was significantly reduced by BU224, a selective I2 imidazoline receptor (IR) blocker, but not by I1 or I3 IR blockers (efaroxan, KU14R respectively). Moreover, the agmatine-induced relaxation was attenuated by glibenclamide and H-89, but enhanced by IBMX. Conclusion: The results suggest that agmatine causes rat prostate relaxation by activation of the I2 IR, which opens KATP channels through cAMP/PKA pathway.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalLUTS: Lower Urinary Tract Symptoms
Volume5
Issue number1
DOIs
Publication statusPublished - Jan 2013

Keywords

  • Adenosine-5'-triphosphate-sensitive K channel
  • Agmatine
  • Imidazoline receptor
  • Prostate

ASJC Scopus subject areas

  • Urology
  • Neurology

Fingerprint Dive into the research topics of 'Agmatine Induces Rat Prostate Relaxation through Activation of Peripheral Imidazoline I2-Receptors'. Together they form a unique fingerprint.

  • Cite this