Ag/Au bi-metallic film based color surface plasmon resonance biosensor with enhanced sensitivity, color contrast and great linearity

Chung Tien Li, Kun Chi Lo, Hsin Yun Chang, Hsieh Ting Wu, Jennifer H. Ho, Ta Jen Yen

Research output: Contribution to journalArticle

18 Citations (Scopus)


In wavelength surface plasmon resonance (SPR) biosensor, the manipulation of SPR dispersion relation by Ag/Au bi-metallic film was first time implemented. Due to the enhanced resonant wavelength shift and the sharper SPR slope of using Ag/Au bi-metallic film, the illuminated color of reflection shows one order of magnitude greater contrast than conventional SPR biosensors. Such an Ag/Au bi-metallic film based color SPR biosensor (CSPRB) allows the detail bio-interactions, for example 100. nM streptavidin, to be distinguished by directly observing the color change of reflection through naked eyes rather than the analysis of spectrometer. In addition to the enhanced sensitivity and color contrast, this CSPRB also possesses a great linear detection range up to 0.0254. RIU, which leading to the application of point-of-care tests.

Original languageEnglish
Pages (from-to)192-198
Number of pages7
JournalBiosensors and Bioelectronics
Issue number1
Publication statusPublished - Jun 2012



  • Bi-metallic film
  • Color contrast
  • Linear detection range
  • Sensitivity
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

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